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Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel

Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel studies are focusing on the use of microalgal lipids as feedstock. Apart from using microalgae for lipid biosynthesis, lipids can also be extracted from other oleaginous microorganisms li...

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Main Authors: Hazmi, Alia Tasnim, Ahmad, Farah B., Athoillah, Ahdyat Zain, Jameel, Ahmad Tariq
Format: Article
Published: Univ Diponegoro 2021
Subjects:
TP Chemical technology
Online Access:http://eprints.um.edu.my/34802/
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spelling my.um.eprints.348022022-05-28T07:44:59Z http://eprints.um.edu.my/34802/ Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel Hazmi, Alia Tasnim Ahmad, Farah B. Athoillah, Ahdyat Zain Jameel, Ahmad Tariq TP Chemical technology Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel studies are focusing on the use of microalgal lipids as feedstock. Apart from using microalgae for lipid biosynthesis, lipids can also be extracted from other oleaginous microorganisms like fungi and yeast. However, there are gaps in the studies of lipid production from filamentous fungi, especially in-situ transesterification process. The aim of this project is to compare in-situ with the ex-situ transesterification of fungal biomass from Aspergillus oryzae. In exsitu transesterification, two methods of lipid extraction, the Soxhlet extraction and the Bligh and Dyer extraction, were performed. For in-situ transesterification, two methods using different catalysts were investigated. Base-catalyzed in-situ transesterification of fungal biomass resulted on the highest Fatty Acid Methyl Esters (FAME) yield. The base-catalyzed in-situ transesterification was further optimized via Central Composite Design (CCD) of Response Surface Methodology (RSM). The parameters investigated were the catalyst loading, methanol to biomass ratio and reaction time. The optimization showed that the highest FAME yield was at 25.1% (w/w) with 10 minutes reaction time, 5% catalyst and 360:1 of the ratio of the methanol to biomass. Based on Analysis of Variance (ANOVA), the model was found to be significant according to the value of ``Prob >F'' of 0.0028. Copyright (C) 2021 by Authors, Published by BCREC Group. Univ Diponegoro 2021-12 Article PeerReviewed Hazmi, Alia Tasnim and Ahmad, Farah B. and Athoillah, Ahdyat Zain and Jameel, Ahmad Tariq (2021) Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel. Bulletin of Chemical Reaction Engineering and Catalysis, 16 (4). pp. 733-743. ISSN 1978-2993, DOI https://doi.org/10.9767/bcrec.16.4.11044.733-743 <https://doi.org/10.9767/bcrec.16.4.11044.733-743>. 10.9767/bcrec.16.4.11044.733-743
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Hazmi, Alia Tasnim
Ahmad, Farah B.
Athoillah, Ahdyat Zain
Jameel, Ahmad Tariq
Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
description Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel studies are focusing on the use of microalgal lipids as feedstock. Apart from using microalgae for lipid biosynthesis, lipids can also be extracted from other oleaginous microorganisms like fungi and yeast. However, there are gaps in the studies of lipid production from filamentous fungi, especially in-situ transesterification process. The aim of this project is to compare in-situ with the ex-situ transesterification of fungal biomass from Aspergillus oryzae. In exsitu transesterification, two methods of lipid extraction, the Soxhlet extraction and the Bligh and Dyer extraction, were performed. For in-situ transesterification, two methods using different catalysts were investigated. Base-catalyzed in-situ transesterification of fungal biomass resulted on the highest Fatty Acid Methyl Esters (FAME) yield. The base-catalyzed in-situ transesterification was further optimized via Central Composite Design (CCD) of Response Surface Methodology (RSM). The parameters investigated were the catalyst loading, methanol to biomass ratio and reaction time. The optimization showed that the highest FAME yield was at 25.1% (w/w) with 10 minutes reaction time, 5% catalyst and 360:1 of the ratio of the methanol to biomass. Based on Analysis of Variance (ANOVA), the model was found to be significant according to the value of ``Prob >F'' of 0.0028. Copyright (C) 2021 by Authors, Published by BCREC Group.
format Article
author Hazmi, Alia Tasnim
Ahmad, Farah B.
Athoillah, Ahdyat Zain
Jameel, Ahmad Tariq
author_facet Hazmi, Alia Tasnim
Ahmad, Farah B.
Athoillah, Ahdyat Zain
Jameel, Ahmad Tariq
author_sort Hazmi, Alia Tasnim
title Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
title_short Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
title_full Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
title_fullStr Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
title_full_unstemmed Comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
title_sort comparison of ex-situ and in situ transesterification for the production of microbial biodiesel
publisher Univ Diponegoro
publishDate 2021
url http://eprints.um.edu.my/34802/
_version_ 1735409621865070592
score 13.211869

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