Staff View: Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties

Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties

The use of ultrasmall gold nanoparticles (usAuNPs, diameters <5 nm) in cancer diagnosis and therapy has been increasing due to their unique tumour tissue/cell penetrating traits and renal-excretable properties. In order to produce usAuNPs with narrow polydispersity, toxic stabilising agents were...

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Main Authors:	Beishenaliev, Adilet, Faruqu, Farid Nazer, Leo, Bey Fen, Lit, Lei Cheng, Loke, Yean Leng, Chang, Chia-Ching, Teo, Yin Yin, Chik, Zamri, Foo, Yiing Yee, Chung, Lip Yong, Kiew, Lik Voon
Format:	Article
Published:	Elsevier 2021
Subjects:	R Medicine
Online Access:	http://eprints.um.edu.my/34421/
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spelling	my.um.eprints.344212022-06-15T04:06:43Z http://eprints.um.edu.my/34421/ Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties Beishenaliev, Adilet Faruqu, Farid Nazer Leo, Bey Fen Lit, Lei Cheng Loke, Yean Leng Chang, Chia-Ching Teo, Yin Yin Chik, Zamri Foo, Yiing Yee Chung, Lip Yong Kiew, Lik Voon R Medicine The use of ultrasmall gold nanoparticles (usAuNPs, diameters <5 nm) in cancer diagnosis and therapy has been increasing due to their unique tumour tissue/cell penetrating traits and renal-excretable properties. In order to produce usAuNPs with narrow polydispersity, toxic stabilising agents were normally coated on usAuNP surface during their synthesis. This has necessitated post-synthesis surface modification to minimise stabilising-agentrelated toxicity in order to qualify usAuNPs for in vivo use. Nevertheless, the average sizes of usAuNPs that were surface-modified using the reported methods were often found to be above the renal filtration threshold (5 nm), hence limiting their renal excretion and suitability for in vivo use. Here, we report a novel and facile synthesis of biocompatible and renal-clearable usAuNPs using sodium alginate as the stabilising agent. The alginate-stabilised usAuNPs (usAuNPs(ALG)) had an average size of 4.5 +/- 0.2 nm with narrow polydispersity (0.21), a zeta potential of -30.4 mV, and were stable in water for up to 45 days. They were haemocompatible and non-toxic in vitro at concentrations up to 100 mu g/ml (4T1 breast cancer cells). Further, usAuNPs(ALG) were found to generate reactive oxygen species and exert dose-dependent sonotoxicity to 4T1 and MDA-MB-231 cells in vitro at a level equivalent to that of the larger AuNPs (>5 nm), but upon ultrasound irradiation at 2-4-fold lower frequency (1 MHz) and intensity (0.5 W/cm(2)) than those previously reported in AuNP-mediated SDT. This suggests the potential use of the usAuNPs(ALG) as sonosensitising agents for anti-cancer adjunctive therapy. Overall, this study reported the novel method for the synthesis of biocompatible usAuNPs with considerable sonosensitising properties and the potential of these sub-5 nm nanoparticles for in vivo applications. Elsevier 2021-10-20 Article PeerReviewed Beishenaliev, Adilet and Faruqu, Farid Nazer and Leo, Bey Fen and Lit, Lei Cheng and Loke, Yean Leng and Chang, Chia-Ching and Teo, Yin Yin and Chik, Zamri and Foo, Yiing Yee and Chung, Lip Yong and Kiew, Lik Voon (2021) Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties. Colloids and Surfaces A-Physicochemical and Engineering Aspects, 627. ISSN 0927-7757, DOI https://doi.org/10.1016/j.colsurfa.2021.127141 <https://doi.org/10.1016/j.colsurfa.2021.127141>. 10.1016/j.colsurfa.2021.127141
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine
spellingShingle	R Medicine Beishenaliev, Adilet Faruqu, Farid Nazer Leo, Bey Fen Lit, Lei Cheng Loke, Yean Leng Chang, Chia-Ching Teo, Yin Yin Chik, Zamri Foo, Yiing Yee Chung, Lip Yong Kiew, Lik Voon Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
description	The use of ultrasmall gold nanoparticles (usAuNPs, diameters <5 nm) in cancer diagnosis and therapy has been increasing due to their unique tumour tissue/cell penetrating traits and renal-excretable properties. In order to produce usAuNPs with narrow polydispersity, toxic stabilising agents were normally coated on usAuNP surface during their synthesis. This has necessitated post-synthesis surface modification to minimise stabilising-agentrelated toxicity in order to qualify usAuNPs for in vivo use. Nevertheless, the average sizes of usAuNPs that were surface-modified using the reported methods were often found to be above the renal filtration threshold (5 nm), hence limiting their renal excretion and suitability for in vivo use. Here, we report a novel and facile synthesis of biocompatible and renal-clearable usAuNPs using sodium alginate as the stabilising agent. The alginate-stabilised usAuNPs (usAuNPs(ALG)) had an average size of 4.5 +/- 0.2 nm with narrow polydispersity (0.21), a zeta potential of -30.4 mV, and were stable in water for up to 45 days. They were haemocompatible and non-toxic in vitro at concentrations up to 100 mu g/ml (4T1 breast cancer cells). Further, usAuNPs(ALG) were found to generate reactive oxygen species and exert dose-dependent sonotoxicity to 4T1 and MDA-MB-231 cells in vitro at a level equivalent to that of the larger AuNPs (>5 nm), but upon ultrasound irradiation at 2-4-fold lower frequency (1 MHz) and intensity (0.5 W/cm(2)) than those previously reported in AuNP-mediated SDT. This suggests the potential use of the usAuNPs(ALG) as sonosensitising agents for anti-cancer adjunctive therapy. Overall, this study reported the novel method for the synthesis of biocompatible usAuNPs with considerable sonosensitising properties and the potential of these sub-5 nm nanoparticles for in vivo applications.
format	Article
author	Beishenaliev, Adilet Faruqu, Farid Nazer Leo, Bey Fen Lit, Lei Cheng Loke, Yean Leng Chang, Chia-Ching Teo, Yin Yin Chik, Zamri Foo, Yiing Yee Chung, Lip Yong Kiew, Lik Voon
author_facet	Beishenaliev, Adilet Faruqu, Farid Nazer Leo, Bey Fen Lit, Lei Cheng Loke, Yean Leng Chang, Chia-Ching Teo, Yin Yin Chik, Zamri Foo, Yiing Yee Chung, Lip Yong Kiew, Lik Voon
author_sort	Beishenaliev, Adilet
title	Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
title_short	Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
title_full	Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
title_fullStr	Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
title_full_unstemmed	Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
title_sort	facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties
publisher	Elsevier
publishDate	2021
url	http://eprints.um.edu.my/34421/
_version_	1736834292132085760
score	13.211869

Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties

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