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Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia

Hand, foot, and mouth disease ( HFMD) is a common childhood disease caused by enteroviruses. In 2018, a HFMD outbreak in Malaysia affected over 76,000 children. In this study, we used RT-qPCR and CODEHOP PCR to detect the causative agents in 89 clinically diagnosed HFMD patients in Kuala Lumpur and...

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Main Authors: Lee, M. H. P., Chong, Y. M., Tay, C. G., Koh, M. T., Chem, Y. K., Noordin, N., Jahis, R., Sam, I. C., Chan, Y. F.
Format: Article
Published: Malaysian Soc Parasitology Tropical Medicine 2021
Subjects:
R Medicine (General)
Online Access:http://eprints.um.edu.my/34208/
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spelling my.um.eprints.342082022-06-14T07:53:29Z http://eprints.um.edu.my/34208/ Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia Lee, M. H. P. Chong, Y. M. Tay, C. G. Koh, M. T. Chem, Y. K. Noordin, N. Jahis, R. Sam, I. C. Chan, Y. F. R Medicine (General) Hand, foot, and mouth disease ( HFMD) is a common childhood disease caused by enteroviruses. In 2018, a HFMD outbreak in Malaysia affected over 76,000 children. In this study, we used RT-qPCR and CODEHOP PCR to detect the causative agents in 89 clinically diagnosed HFMD patients in Kuala Lumpur and Selangor. Most (62.9%) of the children were below 3 years old. PCR with either assay detected enteroviruses in 84.2% (75/89) and CODEHOP PCR successfully typed 66.7% (50/75) of the enteroviruses. Sequencing of CODEHOP amplicons showed co-circulation of multiple enteroviruses with coxsackievirus A6 (CV-A6) and A16 as the predominant serotypes, but not the neurovirulent enterovirus A71. CV-A6 infection was more common in children less than 12 months old (p=0.01) and was more likely to cause vesicles in the gluteal area (p=0.01) compared to other enteroviruses. Establishing a robust identification method during HFMD outbreaks is important for patient management and public health responses. Malaysian Soc Parasitology Tropical Medicine 2021-03 Article PeerReviewed Lee, M. H. P. and Chong, Y. M. and Tay, C. G. and Koh, M. T. and Chem, Y. K. and Noordin, N. and Jahis, R. and Sam, I. C. and Chan, Y. F. (2021) Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia. Tropical Biomedicine, 38 (1). pp. 150-153. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.38.1.026 <https://doi.org/10.47665/tb.38.1.026>. 10.47665/tb.38.1.026
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Lee, M. H. P.
Chong, Y. M.
Tay, C. G.
Koh, M. T.
Chem, Y. K.
Noordin, N.
Jahis, R.
Sam, I. C.
Chan, Y. F.
Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
description Hand, foot, and mouth disease ( HFMD) is a common childhood disease caused by enteroviruses. In 2018, a HFMD outbreak in Malaysia affected over 76,000 children. In this study, we used RT-qPCR and CODEHOP PCR to detect the causative agents in 89 clinically diagnosed HFMD patients in Kuala Lumpur and Selangor. Most (62.9%) of the children were below 3 years old. PCR with either assay detected enteroviruses in 84.2% (75/89) and CODEHOP PCR successfully typed 66.7% (50/75) of the enteroviruses. Sequencing of CODEHOP amplicons showed co-circulation of multiple enteroviruses with coxsackievirus A6 (CV-A6) and A16 as the predominant serotypes, but not the neurovirulent enterovirus A71. CV-A6 infection was more common in children less than 12 months old (p=0.01) and was more likely to cause vesicles in the gluteal area (p=0.01) compared to other enteroviruses. Establishing a robust identification method during HFMD outbreaks is important for patient management and public health responses.
format Article
author Lee, M. H. P.
Chong, Y. M.
Tay, C. G.
Koh, M. T.
Chem, Y. K.
Noordin, N.
Jahis, R.
Sam, I. C.
Chan, Y. F.
author_facet Lee, M. H. P.
Chong, Y. M.
Tay, C. G.
Koh, M. T.
Chem, Y. K.
Noordin, N.
Jahis, R.
Sam, I. C.
Chan, Y. F.
author_sort Lee, M. H. P.
title Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
title_short Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
title_full Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
title_fullStr Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
title_full_unstemmed Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
title_sort detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in malaysia
publisher Malaysian Soc Parasitology Tropical Medicine
publishDate 2021
url http://eprints.um.edu.my/34208/
_version_ 1736834286402666496
score 13.211869

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