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Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo

In March 2018, an algal bloom of Pseudo-nitzschia was detected, for the first time, in a semi-enclosed lagoon in Miri, Sarawak, Malaysia Borneo. The plankton samples were collected for cell enumeration and species identification by electron microscopy and molecular characterization. Liquid chromatog...

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Main Authors: Teng, Sing Tung, Abdullah, Nursyahida, Hanifah, Afiqah Halmiton, Tan, Suh Nih, Gao, Chunlei, Law, Ing Kuo, Leaw, Chui Pin, Lim, Po Teen
Format: Article
Published: Pergamon-Elsevier Science 2021
Subjects:
GC Oceanography
GE Environmental Sciences
Q Science (General)
Online Access:http://eprints.um.edu.my/34123/
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spelling my.um.eprints.341232022-06-22T07:12:56Z http://eprints.um.edu.my/34123/ Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo Teng, Sing Tung Abdullah, Nursyahida Hanifah, Afiqah Halmiton Tan, Suh Nih Gao, Chunlei Law, Ing Kuo Leaw, Chui Pin Lim, Po Teen GC Oceanography GE Environmental Sciences Q Science (General) In March 2018, an algal bloom of Pseudo-nitzschia was detected, for the first time, in a semi-enclosed lagoon in Miri, Sarawak, Malaysia Borneo. The plankton samples were collected for cell enumeration and species identification by electron microscopy and molecular characterization. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to detect and quantify the neurotoxin domoic acid (DA) in both the plankton and shellfish samples. The abundance of Pseudo-nitzschia cells ranged from 5.6 x 10(5) to 3.5 x 10(6) cell L-1 during the bloom event. Morphological observation of the cells by transmission electron microscopy showed that the plankton samples comprised a single Pseudo-nitzschia morphotype resembling P. cuspidata. The ITS2 sequence-structure phylogenetic inference further supported the species identity as Pseudo-nitzschia cuspidata. Low levels of DA were detected in the plankton samples, with cellular DA, particulate DA, and dissolved DA of 257-504 fg DA cell(-1), 676 ng L-1, and 15 ng L-1, respectively. The amount of DA, 8 mu g g(-1) tissue, was found present in the shellfish sample (Magallana sp.) which is below the regulatory limit of 20 mu g DA g(-1) tissue. The study documented, for the first time, DA contamination in shellfish that associated with bloom of P. cuspidata in the Western Pacific region. Pergamon-Elsevier Science 2021-10-30 Article PeerReviewed Teng, Sing Tung and Abdullah, Nursyahida and Hanifah, Afiqah Halmiton and Tan, Suh Nih and Gao, Chunlei and Law, Ing Kuo and Leaw, Chui Pin and Lim, Po Teen (2021) Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo. Toxicon, 202. pp. 132-141. ISSN 0041-0101, DOI https://doi.org/10.1016/j.toxicon.2021.09.018 <https://doi.org/10.1016/j.toxicon.2021.09.018>. 10.1016/j.toxicon.2021.09.018
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic GC Oceanography
GE Environmental Sciences
Q Science (General)
spellingShingle GC Oceanography
GE Environmental Sciences
Q Science (General)
Teng, Sing Tung
Abdullah, Nursyahida
Hanifah, Afiqah Halmiton
Tan, Suh Nih
Gao, Chunlei
Law, Ing Kuo
Leaw, Chui Pin
Lim, Po Teen
Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
description In March 2018, an algal bloom of Pseudo-nitzschia was detected, for the first time, in a semi-enclosed lagoon in Miri, Sarawak, Malaysia Borneo. The plankton samples were collected for cell enumeration and species identification by electron microscopy and molecular characterization. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to detect and quantify the neurotoxin domoic acid (DA) in both the plankton and shellfish samples. The abundance of Pseudo-nitzschia cells ranged from 5.6 x 10(5) to 3.5 x 10(6) cell L-1 during the bloom event. Morphological observation of the cells by transmission electron microscopy showed that the plankton samples comprised a single Pseudo-nitzschia morphotype resembling P. cuspidata. The ITS2 sequence-structure phylogenetic inference further supported the species identity as Pseudo-nitzschia cuspidata. Low levels of DA were detected in the plankton samples, with cellular DA, particulate DA, and dissolved DA of 257-504 fg DA cell(-1), 676 ng L-1, and 15 ng L-1, respectively. The amount of DA, 8 mu g g(-1) tissue, was found present in the shellfish sample (Magallana sp.) which is below the regulatory limit of 20 mu g DA g(-1) tissue. The study documented, for the first time, DA contamination in shellfish that associated with bloom of P. cuspidata in the Western Pacific region.
format Article
author Teng, Sing Tung
Abdullah, Nursyahida
Hanifah, Afiqah Halmiton
Tan, Suh Nih
Gao, Chunlei
Law, Ing Kuo
Leaw, Chui Pin
Lim, Po Teen
author_facet Teng, Sing Tung
Abdullah, Nursyahida
Hanifah, Afiqah Halmiton
Tan, Suh Nih
Gao, Chunlei
Law, Ing Kuo
Leaw, Chui Pin
Lim, Po Teen
author_sort Teng, Sing Tung
title Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
title_short Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
title_full Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
title_fullStr Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
title_full_unstemmed Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo
title_sort toxic bloom of pseudo-nitzschia cuspidata (bacillariophyceae) and domoic acid contamination of bivalve molluscs in malaysia borneo
publisher Pergamon-Elsevier Science
publishDate 2021
url http://eprints.um.edu.my/34123/
_version_ 1738510710291300352
score 13.244367

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