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Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies

Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resoluti...

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Main Authors: Nordin, Nurdiana, Bordonali, Lorenzo, Davoodi, Hossein, Ratnawati, Novindi Dwi, Gygli, Gudrun, Korvink, Jan G., Badilita, Vlad, MacKinnon, Neil
Format: Article
Published: Wiley-V C H Verlag GMBH 2021
Subjects:
QD Chemistry
Online Access:http://eprints.um.edu.my/34001/
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spelling my.um.eprints.340012022-06-27T01:38:16Z http://eprints.um.edu.my/34001/ Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies Nordin, Nurdiana Bordonali, Lorenzo Davoodi, Hossein Ratnawati, Novindi Dwi Gygli, Gudrun Korvink, Jan G. Badilita, Vlad MacKinnon, Neil QD Chemistry Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real-time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled. Wiley-V C H Verlag GMBH 2021-08-23 Article PeerReviewed Nordin, Nurdiana and Bordonali, Lorenzo and Davoodi, Hossein and Ratnawati, Novindi Dwi and Gygli, Gudrun and Korvink, Jan G. and Badilita, Vlad and MacKinnon, Neil (2021) Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies. Angewandte Chemie-International Edition, 60 (35). pp. 19176-19182. ISSN 1433-7851, DOI https://doi.org/10.1002/anie.202103585 <https://doi.org/10.1002/anie.202103585>. 10.1002/anie.202103585
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
spellingShingle QD Chemistry
Nordin, Nurdiana
Bordonali, Lorenzo
Davoodi, Hossein
Ratnawati, Novindi Dwi
Gygli, Gudrun
Korvink, Jan G.
Badilita, Vlad
MacKinnon, Neil
Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
description Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real-time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.
format Article
author Nordin, Nurdiana
Bordonali, Lorenzo
Davoodi, Hossein
Ratnawati, Novindi Dwi
Gygli, Gudrun
Korvink, Jan G.
Badilita, Vlad
MacKinnon, Neil
author_facet Nordin, Nurdiana
Bordonali, Lorenzo
Davoodi, Hossein
Ratnawati, Novindi Dwi
Gygli, Gudrun
Korvink, Jan G.
Badilita, Vlad
MacKinnon, Neil
author_sort Nordin, Nurdiana
title Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
title_short Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
title_full Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
title_fullStr Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
title_full_unstemmed Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
title_sort real-time nmr monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies
publisher Wiley-V C H Verlag GMBH
publishDate 2021
url http://eprints.um.edu.my/34001/
_version_ 1738510699432247296
score 13.211869

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