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Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells

BACKGROUND: The plasma cholesterol and triacylglycerol lowering effects of Tamarindus indica extract have been previously described. We have also shown that the methanol extract of T. indica fruit pulp altered the expression of lipid-associated genes including ABCG5 and APOAI in HepG2 cells. In the...

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Main Authors: Chong, U.R., Abdul-Rahman, P.S., Abdul-Aziz, A., Hashim, Onn Haji, Junit, Sarni Mat
Format: Article
Published: Public Library of Science 2012
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/3399/
http://www.ncbi.nlm.nih.gov/pubmed/22724021
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spelling my.um.eprints.33992019-10-26T04:44:11Z http://eprints.um.edu.my/3399/ Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells Chong, U.R. Abdul-Rahman, P.S. Abdul-Aziz, A. Hashim, Onn Haji Junit, Sarni Mat R Medicine BACKGROUND: The plasma cholesterol and triacylglycerol lowering effects of Tamarindus indica extract have been previously described. We have also shown that the methanol extract of T. indica fruit pulp altered the expression of lipid-associated genes including ABCG5 and APOAI in HepG2 cells. In the present study, effects of the same extract on the release of proteins from the cells were investigated using the proteomics approach. METHODOLOGY/PRINCIPAL FINDINGS: When culture media of HepG2 cells grown in the absence and presence of the methanol extract of T. indica fruit pulp were subjected to 2-dimensional gel electrophoresis, the expression of seven proteins was found to be significantly different (p<0.03125). Five of the spots were subsequently identified as alpha enolase (ENO1), transthyretin (TTR), apolipoprotein A-I (ApoA-I; two isoforms), and rab GDP dissociation inhibitor beta (GDI-2). A functional network of lipid metabolism, molecular transport and small molecule biochemistry that interconnects the three latter proteins with the interactomes was identified using the Ingenuity Pathways Analysis software. CONCLUSION/SIGNIFICANCE: The methanol extract of T. indica fruit pulp altered the release of ENO1, ApoA-I, TTR and GDI-2 from HepG2 cells. Our results provide support on the effect of T. indica extract on cellular lipid metabolism, particularly that of cholesterol. Public Library of Science 2012 Article PeerReviewed Chong, U.R. and Abdul-Rahman, P.S. and Abdul-Aziz, A. and Hashim, Onn Haji and Junit, Sarni Mat (2012) Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells. PLoS ONE, 7 (6). ISSN 1932-6203 http://www.ncbi.nlm.nih.gov/pubmed/22724021 PMID: 22724021
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Chong, U.R.
Abdul-Rahman, P.S.
Abdul-Aziz, A.
Hashim, Onn Haji
Junit, Sarni Mat
Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
description BACKGROUND: The plasma cholesterol and triacylglycerol lowering effects of Tamarindus indica extract have been previously described. We have also shown that the methanol extract of T. indica fruit pulp altered the expression of lipid-associated genes including ABCG5 and APOAI in HepG2 cells. In the present study, effects of the same extract on the release of proteins from the cells were investigated using the proteomics approach. METHODOLOGY/PRINCIPAL FINDINGS: When culture media of HepG2 cells grown in the absence and presence of the methanol extract of T. indica fruit pulp were subjected to 2-dimensional gel electrophoresis, the expression of seven proteins was found to be significantly different (p<0.03125). Five of the spots were subsequently identified as alpha enolase (ENO1), transthyretin (TTR), apolipoprotein A-I (ApoA-I; two isoforms), and rab GDP dissociation inhibitor beta (GDI-2). A functional network of lipid metabolism, molecular transport and small molecule biochemistry that interconnects the three latter proteins with the interactomes was identified using the Ingenuity Pathways Analysis software. CONCLUSION/SIGNIFICANCE: The methanol extract of T. indica fruit pulp altered the release of ENO1, ApoA-I, TTR and GDI-2 from HepG2 cells. Our results provide support on the effect of T. indica extract on cellular lipid metabolism, particularly that of cholesterol.
format Article
author Chong, U.R.
Abdul-Rahman, P.S.
Abdul-Aziz, A.
Hashim, Onn Haji
Junit, Sarni Mat
author_facet Chong, U.R.
Abdul-Rahman, P.S.
Abdul-Aziz, A.
Hashim, Onn Haji
Junit, Sarni Mat
author_sort Chong, U.R.
title Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
title_short Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
title_full Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
title_fullStr Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
title_full_unstemmed Tamarindus indica extract alters release of alpha enolase, apolipoprotein A-I, transthyretin and rab GDP dissociation inhibitor beta from HepG2 cells
title_sort tamarindus indica extract alters release of alpha enolase, apolipoprotein a-i, transthyretin and rab gdp dissociation inhibitor beta from hepg2 cells
publisher Public Library of Science
publishDate 2012
url http://eprints.um.edu.my/3399/
http://www.ncbi.nlm.nih.gov/pubmed/22724021
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score 13.211869

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