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Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma

Background. Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to...

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Main Authors:	Wong, Yin-Ling, Ramanathan, Anand, Yuen, Kar Mun, Mustafa, Wan Mahadzir Wan, Abraham, Mannil Thomas, Tay, Keng Kiong, Rahman, Zainal Ariff Abdul, Chen, Yeng
Format:	Article
Published:	PeerJ Inc 2021
Subjects:	RK Dentistry Oral surger
Online Access:	http://eprints.um.edu.my/33914/
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spelling	my.um.eprints.339142022-07-13T03:23:05Z http://eprints.um.edu.my/33914/ Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma Wong, Yin-Ling Ramanathan, Anand Yuen, Kar Mun Mustafa, Wan Mahadzir Wan Abraham, Mannil Thomas Tay, Keng Kiong Rahman, Zainal Ariff Abdul Chen, Yeng RK Dentistry Oral surger Background. Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods. Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results. The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0:05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion. Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation. PeerJ Inc 2021-06-10 Article PeerReviewed Wong, Yin-Ling and Ramanathan, Anand and Yuen, Kar Mun and Mustafa, Wan Mahadzir Wan and Abraham, Mannil Thomas and Tay, Keng Kiong and Rahman, Zainal Ariff Abdul and Chen, Yeng (2021) Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma. PeerJ, 9. ISSN 2167-8359, DOI https://doi.org/10.7717/peerj.11548 <https://doi.org/10.7717/peerj.11548>. 10.7717/peerj.11548
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	RK Dentistry Oral surger
spellingShingle	RK Dentistry Oral surger Wong, Yin-Ling Ramanathan, Anand Yuen, Kar Mun Mustafa, Wan Mahadzir Wan Abraham, Mannil Thomas Tay, Keng Kiong Rahman, Zainal Ariff Abdul Chen, Yeng Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
description	Background. Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods. Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results. The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0:05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion. Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation.
format	Article
author	Wong, Yin-Ling Ramanathan, Anand Yuen, Kar Mun Mustafa, Wan Mahadzir Wan Abraham, Mannil Thomas Tay, Keng Kiong Rahman, Zainal Ariff Abdul Chen, Yeng
author_facet	Wong, Yin-Ling Ramanathan, Anand Yuen, Kar Mun Mustafa, Wan Mahadzir Wan Abraham, Mannil Thomas Tay, Keng Kiong Rahman, Zainal Ariff Abdul Chen, Yeng
author_sort	Wong, Yin-Ling
title	Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
title_short	Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
title_full	Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
title_fullStr	Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
title_full_unstemmed	Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
title_sort	comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
publisher	PeerJ Inc
publishDate	2021
url	http://eprints.um.edu.my/33914/
_version_	1738510690843361280
score	13.211869

Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma

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