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Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense

Ganoderma boninense is the most pathogenic fungal species for the oil palm. The molecular interactions involved in its pathogenicity remains undefined. By comparing the proteome profiles of a pathogenic and non-pathogenic species of Ganoderma, the identities of proteins essential for pathogenicity m...

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Main Authors: Dzulkafli, Shahirah Balqis, Othman, Abrizah, Lau, Benjamin Yii Chung, Ramli, Umi Salamah, Karsani, Saiful Anuar
Format: Article
Published: Academic Press 2022
Subjects:
G Geography. Anthropology. Recreation
GE Environmental Sciences
QK Botany
Online Access:http://eprints.um.edu.my/33678/
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spelling my.um.eprints.336782022-07-25T06:46:01Z http://eprints.um.edu.my/33678/ Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense Dzulkafli, Shahirah Balqis Othman, Abrizah Lau, Benjamin Yii Chung Ramli, Umi Salamah Karsani, Saiful Anuar G Geography. Anthropology. Recreation GE Environmental Sciences QK Botany Ganoderma boninense is the most pathogenic fungal species for the oil palm. The molecular interactions involved in its pathogenicity remains undefined. By comparing the proteome profiles of a pathogenic and non-pathogenic species of Ganoderma, the identities of proteins essential for pathogenicity may be identified. As such we performed a shotgun proteomic analysis comparing G. boninense and G. tornatum. We were able to identify 1645 proteins, out of which 128 proteins and 187 proteins were significantly increased in abundance in G. boninense and G. tornatum, respectively, under carbon and nitrogen starvation with and without the presence of the oil palm sawdust (conditions that mimicked field conditions). A bioinformatics analysis of these proteins suggested that during carbon and nitrogen starvation, groups of proteins related to catalytic activity, binding, cellular anatomical entity, metabolic process and cellular process underwent extensive alterations in both Ganoderma species. In addition, exposure to carbon and nitrogen starvation with the presence of the oil palm sawdust, induced different groups of proteins in G. boninense and G. tornatum that may be linked to the different level of pathogenicity between both Ganoderma species in oil palm. Academic Press 2022-01 Article PeerReviewed Dzulkafli, Shahirah Balqis and Othman, Abrizah and Lau, Benjamin Yii Chung and Ramli, Umi Salamah and Karsani, Saiful Anuar (2022) Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense. Physiological and Molecular Plant Pathology, 117. ISSN 0885-5765, DOI https://doi.org/10.1016/j.pmpp.2021.101736 <https://doi.org/10.1016/j.pmpp.2021.101736>. 10.1016/j.pmpp.2021.101736
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic G Geography. Anthropology. Recreation
GE Environmental Sciences
QK Botany
spellingShingle G Geography. Anthropology. Recreation
GE Environmental Sciences
QK Botany
Dzulkafli, Shahirah Balqis
Othman, Abrizah
Lau, Benjamin Yii Chung
Ramli, Umi Salamah
Karsani, Saiful Anuar
Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
description Ganoderma boninense is the most pathogenic fungal species for the oil palm. The molecular interactions involved in its pathogenicity remains undefined. By comparing the proteome profiles of a pathogenic and non-pathogenic species of Ganoderma, the identities of proteins essential for pathogenicity may be identified. As such we performed a shotgun proteomic analysis comparing G. boninense and G. tornatum. We were able to identify 1645 proteins, out of which 128 proteins and 187 proteins were significantly increased in abundance in G. boninense and G. tornatum, respectively, under carbon and nitrogen starvation with and without the presence of the oil palm sawdust (conditions that mimicked field conditions). A bioinformatics analysis of these proteins suggested that during carbon and nitrogen starvation, groups of proteins related to catalytic activity, binding, cellular anatomical entity, metabolic process and cellular process underwent extensive alterations in both Ganoderma species. In addition, exposure to carbon and nitrogen starvation with the presence of the oil palm sawdust, induced different groups of proteins in G. boninense and G. tornatum that may be linked to the different level of pathogenicity between both Ganoderma species in oil palm.
format Article
author Dzulkafli, Shahirah Balqis
Othman, Abrizah
Lau, Benjamin Yii Chung
Ramli, Umi Salamah
Karsani, Saiful Anuar
author_facet Dzulkafli, Shahirah Balqis
Othman, Abrizah
Lau, Benjamin Yii Chung
Ramli, Umi Salamah
Karsani, Saiful Anuar
author_sort Dzulkafli, Shahirah Balqis
title Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
title_short Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
title_full Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
title_fullStr Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
title_full_unstemmed Comparative proteome analysis of mycelial proteins from G. boninense vs G. tornatum: Identification of proteins potentially involved in the pathogenicity of G. boninense
title_sort comparative proteome analysis of mycelial proteins from g. boninense vs g. tornatum: identification of proteins potentially involved in the pathogenicity of g. boninense
publisher Academic Press
publishDate 2022
url http://eprints.um.edu.my/33678/
_version_ 1739828469313830912
score 13.211869

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