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In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics

In the current study, sustained release salicylic acid (SA) and ketoconazole (KCZ) loaded silica nanoparticles (SiO2-NPs) were encapsulated in natural macromolecule-alginate (ALG) based scaffold through freeze gelation method for an effective treatment of commonly prevailed fatal fungal infections....

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Main Authors:	Masood, Syeda Amna, Maheen, Safirah, Khan, Hafeez Ullah, Zafar, Muhammad Nadeem, Shafqat, Syed Salman, Mujtaba, M. A., Rehman, Atta Ur, Abbas, Ghulam, Mahmood, Mian H. R., Bashir, Shahid, Khan, T. M. Yunus, Khalifa, Amany Salah
Format:	Article
Published:	Elsevier 2022
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://eprints.um.edu.my/33636/
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spelling	my.um.eprints.336362022-07-27T08:23:23Z http://eprints.um.edu.my/33636/ In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics Masood, Syeda Amna Maheen, Safirah Khan, Hafeez Ullah Zafar, Muhammad Nadeem Shafqat, Syed Salman Mujtaba, M. A. Rehman, Atta Ur Abbas, Ghulam Mahmood, Mian H. R. Bashir, Shahid Khan, T. M. Yunus Khalifa, Amany Salah TA Engineering (General). Civil engineering (General) In the current study, sustained release salicylic acid (SA) and ketoconazole (KCZ) loaded silica nanoparticles (SiO2-NPs) were encapsulated in natural macromolecule-alginate (ALG) based scaffold through freeze gelation method for an effective treatment of commonly prevailed fatal fungal infections. After statistical optimization by central composite rotatable design (CCRD), the optimized scaffold was subjected to comparative in vitro/in vivo antifungal, skin irritation, wound healing, cytotoxicity, and histopathological evaluations. In physico-chemical characterization performed through X-ray diffraction (p-XRD), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA), an absolute lack of structural interactions was found between drugs and formulation components. The zeta potential and scanning electron microscopy (SEM) revealed spherical, highly porous negatively charged (-23.1) SiO2-NPs having a size distribution of 40-80 nm with successful encapsulation in negatively charged scaffold (-20.2 mV). The entrapment efficiency and drugs release exhibited visible quadratic influence of formulation variables on scaffold. The optimized ALG-scaffold demonstrated comparatively an enhanced in vitro, in vivo antifungal activity, least cytotoxicity and rapid wound healing efficacy in histopathological evaluation by sustained drugs release up to 14-days without any skin irritation effect. The study suggested the potential of alginate scaffold for not only the endurance of drugs loaded SiO2-NPs but also for the simultaneous co-delivery of medicaments fulfilling the need of consistent prolonged availability of drugs for better fungal therapeutics. (C) 2021 THE AUTHORS. Published by Elsevier BV on behalf of Faculty of Engineering, Alexandria University. Elsevier 2022-05 Article PeerReviewed Masood, Syeda Amna and Maheen, Safirah and Khan, Hafeez Ullah and Zafar, Muhammad Nadeem and Shafqat, Syed Salman and Mujtaba, M. A. and Rehman, Atta Ur and Abbas, Ghulam and Mahmood, Mian H. R. and Bashir, Shahid and Khan, T. M. Yunus and Khalifa, Amany Salah (2022) In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics. Alexandria Engineering Journal, 61 (5). pp. 4041-4056. ISSN 1110-0168, DOI https://doi.org/10.1016/j.aej.2021.09.027 <https://doi.org/10.1016/j.aej.2021.09.027>. 10.1016/j.aej.2021.09.027
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Masood, Syeda Amna Maheen, Safirah Khan, Hafeez Ullah Zafar, Muhammad Nadeem Shafqat, Syed Salman Mujtaba, M. A. Rehman, Atta Ur Abbas, Ghulam Mahmood, Mian H. R. Bashir, Shahid Khan, T. M. Yunus Khalifa, Amany Salah In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
description	In the current study, sustained release salicylic acid (SA) and ketoconazole (KCZ) loaded silica nanoparticles (SiO2-NPs) were encapsulated in natural macromolecule-alginate (ALG) based scaffold through freeze gelation method for an effective treatment of commonly prevailed fatal fungal infections. After statistical optimization by central composite rotatable design (CCRD), the optimized scaffold was subjected to comparative in vitro/in vivo antifungal, skin irritation, wound healing, cytotoxicity, and histopathological evaluations. In physico-chemical characterization performed through X-ray diffraction (p-XRD), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA), an absolute lack of structural interactions was found between drugs and formulation components. The zeta potential and scanning electron microscopy (SEM) revealed spherical, highly porous negatively charged (-23.1) SiO2-NPs having a size distribution of 40-80 nm with successful encapsulation in negatively charged scaffold (-20.2 mV). The entrapment efficiency and drugs release exhibited visible quadratic influence of formulation variables on scaffold. The optimized ALG-scaffold demonstrated comparatively an enhanced in vitro, in vivo antifungal activity, least cytotoxicity and rapid wound healing efficacy in histopathological evaluation by sustained drugs release up to 14-days without any skin irritation effect. The study suggested the potential of alginate scaffold for not only the endurance of drugs loaded SiO2-NPs but also for the simultaneous co-delivery of medicaments fulfilling the need of consistent prolonged availability of drugs for better fungal therapeutics. (C) 2021 THE AUTHORS. Published by Elsevier BV on behalf of Faculty of Engineering, Alexandria University.
format	Article
author	Masood, Syeda Amna Maheen, Safirah Khan, Hafeez Ullah Zafar, Muhammad Nadeem Shafqat, Syed Salman Mujtaba, M. A. Rehman, Atta Ur Abbas, Ghulam Mahmood, Mian H. R. Bashir, Shahid Khan, T. M. Yunus Khalifa, Amany Salah
author_facet	Masood, Syeda Amna Maheen, Safirah Khan, Hafeez Ullah Zafar, Muhammad Nadeem Shafqat, Syed Salman Mujtaba, M. A. Rehman, Atta Ur Abbas, Ghulam Mahmood, Mian H. R. Bashir, Shahid Khan, T. M. Yunus Khalifa, Amany Salah
author_sort	Masood, Syeda Amna
title	In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
title_short	In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
title_full	In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
title_fullStr	In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
title_full_unstemmed	In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
title_sort	in vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics
publisher	Elsevier
publishDate	2022
url	http://eprints.um.edu.my/33636/
_version_	1739828463258304512
score	13.211869

In vitro/in vivo evaluation of statistically engineered alginate scaffold reinforced with dual drugs loaded silica nanoparticles for enhanced fungal therapeutics

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