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Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay

There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity mor...

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Main Authors: Tay, S.T., Devi, S., Puthucheary, S.D., Kautner, I.M.
Format: Article
Published: Microbiology Society 1995
Subjects:
R Medicine (General)
Online Access:http://eprints.um.edu.my/334/
http://www.ncbi.nlm.nih.gov/pubmed/7884798
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spelling my.um.eprints.3342019-04-18T07:56:39Z http://eprints.um.edu.my/334/ Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay Tay, S.T. Devi, S. Puthucheary, S.D. Kautner, I.M. R Medicine (General) There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity more clearly. The incubation conditions (temperature and gaseous) appear to be important for the expression of this activity. Ninety four percent of the Campylobacter isolates examined were found to be haemolytic by the microplate assay with minimal haemolytic units that ranged from 1 to 64. Haemolytic activity was detected only from live bacterial cultures and not from any of the 50 bacterial culture supernates, which suggests that campylobacters may possess a cell-associated haemolysin. The identification of such haemolytic activity in a large number of campylobacters (94%) suggests its potential role as a virulence factor in campylobacter gastroenteritis. Microbiology Society 1995-03 Article PeerReviewed Tay, S.T. and Devi, S. and Puthucheary, S.D. and Kautner, I.M. (1995) Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay. Journal of Medical Microbiology, 42 (3). pp. 175-80. ISSN 0022-2615 http://www.ncbi.nlm.nih.gov/pubmed/7884798 7884798
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Tay, S.T.
Devi, S.
Puthucheary, S.D.
Kautner, I.M.
Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
description There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity more clearly. The incubation conditions (temperature and gaseous) appear to be important for the expression of this activity. Ninety four percent of the Campylobacter isolates examined were found to be haemolytic by the microplate assay with minimal haemolytic units that ranged from 1 to 64. Haemolytic activity was detected only from live bacterial cultures and not from any of the 50 bacterial culture supernates, which suggests that campylobacters may possess a cell-associated haemolysin. The identification of such haemolytic activity in a large number of campylobacters (94%) suggests its potential role as a virulence factor in campylobacter gastroenteritis.
format Article
author Tay, S.T.
Devi, S.
Puthucheary, S.D.
Kautner, I.M.
author_facet Tay, S.T.
Devi, S.
Puthucheary, S.D.
Kautner, I.M.
author_sort Tay, S.T.
title Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
title_short Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
title_full Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
title_fullStr Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
title_full_unstemmed Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
title_sort detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
publisher Microbiology Society
publishDate 1995
url http://eprints.um.edu.my/334/
http://www.ncbi.nlm.nih.gov/pubmed/7884798
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score 13.211869

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