Staff View: A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia

A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia

Background: Carbapenem resistant Enterobacteriaceae (CRE) has rapidly disseminated worldwide and has become a global threat to the healthcare system due to its resistance towards ``last line'' antibiotics. This study aimed to investigate the prevalence of CRE and the resistance mechanism a...

Full description

Saved in:

Bibliographic Details
Main Authors:	Kong, Zhi Xian, Karunakaran, Rina N., Jabar, Kartini Abdul, Ponnampalavanar, Sasheela, Chong, Chun Wie, Teh, Cindy Shuan Ju
Format:	Article
Published:	PeerJ Inc 2022
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://eprints.um.edu.my/33342/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.33342
record_format	eprints
spelling	my.um.eprints.333422022-08-09T06:57:00Z http://eprints.um.edu.my/33342/ A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia Kong, Zhi Xian Karunakaran, Rina N. Jabar, Kartini Abdul Ponnampalavanar, Sasheela Chong, Chun Wie Teh, Cindy Shuan Ju Q Science (General) T Technology (General) Background: Carbapenem resistant Enterobacteriaceae (CRE) has rapidly disseminated worldwide and has become a global threat to the healthcare system due to its resistance towards ``last line'' antibiotics. This study aimed to investigate the prevalence of CRE and the resistance mechanism as well as the risk factors associated with in-hospital mortality. Methods: A total of 168 CRE strains isolated from a tertiary teaching hospital from 2014-2015 were included in this study. The presence of carbapenemase genes and minimum inhibitory concentration of imipenem, meropenem and colistin were investigated. All carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains were characterised by PFGE. The risk factors of patients infected by CRE associated with in-hospital mortality were determined statistically. Results: The predominant CRE species isolated was K. pneumoniae. The carbapenemases detected were blaOXA-48, blaOXA-232, blaVIM and blaNDM of which blaOXA-48 was the predominant carbapenemase detected among 168 CRE strains. A total of 40 CRE strains harboured two different carbapenemase genes. A total of seven clusters and 48 pulsotypes were identified among 140 CRKp strains. A predominant pulsotype responsible for the transmission from 2014 to 2015 was identified. Univariate statistical analysis identified that the period between CRE isolation and start of appropriate therapy of more than 3 days was statistically associated with in-hospital mortality. PeerJ Inc 2022-02-22 Article PeerReviewed Kong, Zhi Xian and Karunakaran, Rina N. and Jabar, Kartini Abdul and Ponnampalavanar, Sasheela and Chong, Chun Wie and Teh, Cindy Shuan Ju (2022) A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia. PeerJ, 10. ISSN 2167-8359, DOI https://doi.org/10.7717/peerj.12830 <https://doi.org/10.7717/peerj.12830>. 10.7717/peerj.12830
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Kong, Zhi Xian Karunakaran, Rina N. Jabar, Kartini Abdul Ponnampalavanar, Sasheela Chong, Chun Wie Teh, Cindy Shuan Ju A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
description	Background: Carbapenem resistant Enterobacteriaceae (CRE) has rapidly disseminated worldwide and has become a global threat to the healthcare system due to its resistance towards ``last line'' antibiotics. This study aimed to investigate the prevalence of CRE and the resistance mechanism as well as the risk factors associated with in-hospital mortality. Methods: A total of 168 CRE strains isolated from a tertiary teaching hospital from 2014-2015 were included in this study. The presence of carbapenemase genes and minimum inhibitory concentration of imipenem, meropenem and colistin were investigated. All carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains were characterised by PFGE. The risk factors of patients infected by CRE associated with in-hospital mortality were determined statistically. Results: The predominant CRE species isolated was K. pneumoniae. The carbapenemases detected were blaOXA-48, blaOXA-232, blaVIM and blaNDM of which blaOXA-48 was the predominant carbapenemase detected among 168 CRE strains. A total of 40 CRE strains harboured two different carbapenemase genes. A total of seven clusters and 48 pulsotypes were identified among 140 CRKp strains. A predominant pulsotype responsible for the transmission from 2014 to 2015 was identified. Univariate statistical analysis identified that the period between CRE isolation and start of appropriate therapy of more than 3 days was statistically associated with in-hospital mortality.
format	Article
author	Kong, Zhi Xian Karunakaran, Rina N. Jabar, Kartini Abdul Ponnampalavanar, Sasheela Chong, Chun Wie Teh, Cindy Shuan Ju
author_facet	Kong, Zhi Xian Karunakaran, Rina N. Jabar, Kartini Abdul Ponnampalavanar, Sasheela Chong, Chun Wie Teh, Cindy Shuan Ju
author_sort	Kong, Zhi Xian
title	A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
title_short	A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
title_full	A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
title_fullStr	A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
title_full_unstemmed	A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia
title_sort	retrospective study on molecular epidemiology trends of carbapenem resistant enterobacteriaceae in a teaching hospital in malaysia
publisher	PeerJ Inc
publishDate	2022
url	http://eprints.um.edu.my/33342/
_version_	1740826023053754368
score	13.211869

A retrospective study on molecular epidemiology trends of carbapenem resistant Enterobacteriaceae in a teaching hospital in Malaysia

Similar Items