Staff View: Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Isolation of exosome from culture medium in an effective way is desired for a less time consuming, cost saving technology in running the diagnostic test on cancer. In this study, we aim to develop an inertial microfluidic channel to separate the nano-size exosome from C666-1 cell culture medium as a...

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Main Authors:	Teoh, Boon Yew, Lim, Yang Mooi, Chong, Wu Yi, Subramaniam, Menaga, Tan, Zi Zhang, Misran, Misni, Suk, Vicit Rizal Eh, Lo, Kwok-Wai, Lee, Poh Foong
Format:	Article
Published:	Springer 2022
Subjects:	QC Physics R Medicine (General) TA Engineering (General). Civil engineering (General)
Online Access:	http://eprints.um.edu.my/33330/
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spelling	my.um.eprints.333302022-08-10T01:41:55Z http://eprints.um.edu.my/33330/ Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel Teoh, Boon Yew Lim, Yang Mooi Chong, Wu Yi Subramaniam, Menaga Tan, Zi Zhang Misran, Misni Suk, Vicit Rizal Eh Lo, Kwok-Wai Lee, Poh Foong QC Physics R Medicine (General) TA Engineering (General). Civil engineering (General) Isolation of exosome from culture medium in an effective way is desired for a less time consuming, cost saving technology in running the diagnostic test on cancer. In this study, we aim to develop an inertial microfluidic channel to separate the nano-size exosome from C666-1 cell culture medium as a selective sample. Simulation was carried out to obtain the optimum flow rate for determining the dimension of the channels for the exosome separation from the medium. The optimal dimension was then brought forward for the actual microfluidic channel fabrication, which consisted of the stages of mask printing, SU8 mould fabrication and ended with PDMS microchannel curing process. The prototype was then used to verify the optimum flow rate with polystyrene particles for its capabilities in actual task on particle separation as a control outcome. Next, the microchip was employed to separate the selected samples, exosome from the culture medium and compared the outcome from the conventional exosome extraction kit to study the level of effectiveness of the prototype. The exosome outcome from both the prototype and extraction kits were characterized through zetasizer, western blot and Transmission electron microscopy (TEM). The microfluidic chip designed in this study obtained a successful separation of exosome from the culture medium. Besides, the extra benefit from this microfluidic channels in particle separation brought an evenly distributed exosome upon collection while the exosomes separated through extraction kit was found clustered together. Therefore, this work has shown the microfluidic channel is suitable for continuous separation of exosome from the culture medium for a clinical study in the future. Springer 2022-03 Article PeerReviewed Teoh, Boon Yew and Lim, Yang Mooi and Chong, Wu Yi and Subramaniam, Menaga and Tan, Zi Zhang and Misran, Misni and Suk, Vicit Rizal Eh and Lo, Kwok-Wai and Lee, Poh Foong (2022) Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel. Biomedical Microdevices, 24 (1). ISSN 1387-2176, DOI https://doi.org/10.1007/s10544-022-00609-z <https://doi.org/10.1007/s10544-022-00609-z>. 10.1007/s10544-022-00609-z
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QC Physics R Medicine (General) TA Engineering (General). Civil engineering (General)
spellingShingle	QC Physics R Medicine (General) TA Engineering (General). Civil engineering (General) Teoh, Boon Yew Lim, Yang Mooi Chong, Wu Yi Subramaniam, Menaga Tan, Zi Zhang Misran, Misni Suk, Vicit Rizal Eh Lo, Kwok-Wai Lee, Poh Foong Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
description	Isolation of exosome from culture medium in an effective way is desired for a less time consuming, cost saving technology in running the diagnostic test on cancer. In this study, we aim to develop an inertial microfluidic channel to separate the nano-size exosome from C666-1 cell culture medium as a selective sample. Simulation was carried out to obtain the optimum flow rate for determining the dimension of the channels for the exosome separation from the medium. The optimal dimension was then brought forward for the actual microfluidic channel fabrication, which consisted of the stages of mask printing, SU8 mould fabrication and ended with PDMS microchannel curing process. The prototype was then used to verify the optimum flow rate with polystyrene particles for its capabilities in actual task on particle separation as a control outcome. Next, the microchip was employed to separate the selected samples, exosome from the culture medium and compared the outcome from the conventional exosome extraction kit to study the level of effectiveness of the prototype. The exosome outcome from both the prototype and extraction kits were characterized through zetasizer, western blot and Transmission electron microscopy (TEM). The microfluidic chip designed in this study obtained a successful separation of exosome from the culture medium. Besides, the extra benefit from this microfluidic channels in particle separation brought an evenly distributed exosome upon collection while the exosomes separated through extraction kit was found clustered together. Therefore, this work has shown the microfluidic channel is suitable for continuous separation of exosome from the culture medium for a clinical study in the future.
format	Article
author	Teoh, Boon Yew Lim, Yang Mooi Chong, Wu Yi Subramaniam, Menaga Tan, Zi Zhang Misran, Misni Suk, Vicit Rizal Eh Lo, Kwok-Wai Lee, Poh Foong
author_facet	Teoh, Boon Yew Lim, Yang Mooi Chong, Wu Yi Subramaniam, Menaga Tan, Zi Zhang Misran, Misni Suk, Vicit Rizal Eh Lo, Kwok-Wai Lee, Poh Foong
author_sort	Teoh, Boon Yew
title	Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
title_short	Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
title_full	Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
title_fullStr	Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
title_full_unstemmed	Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel
title_sort	isolation of exosome from the culture medium of nasopharyngeal cancer (npc) c666-1 cells using inertial based microfluidic channel
publisher	Springer
publishDate	2022
url	http://eprints.um.edu.my/33330/
_version_	1740826022371131392
score	13.211869

Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

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