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Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population

BACKGROUND: Human platelet antigens (HPA) are determinant in several platelet-specific alloimmune disorders, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet transfusion refractoriness. The distribution of HPA systems in the Malaysian population is not known. Defi...

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Main Authors: Tan, J.Y., Lian, L.H., Nadarajan, V.S.
Format: Article
Published: 2012
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/3310/
http://www.ncbi.nlm.nih.gov/pubmed/22682339
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spelling my.um.eprints.33102014-12-30T02:27:55Z http://eprints.um.edu.my/3310/ Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population Tan, J.Y. Lian, L.H. Nadarajan, V.S. R Medicine BACKGROUND: Human platelet antigens (HPA) are determinant in several platelet-specific alloimmune disorders, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet transfusion refractoriness. The distribution of HPA systems in the Malaysian population is not known. Defining the patterns of HPA systems provides a basis for risk assessment and management of the above complications. MATERIALS AND METHODS: The aim of this study was to investigate the distribution of HPA -1 to -6 and -15 in the three major ethnic groups (Malay, Chinese and Indian) in the Malaysian population. A total of 600 random donor samples, 200 from each of the three ethnic groups, were genotyped by means of real time polymerase chain reaction (PCR) with hydrolysis probes and PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS: The most common genotype observed in this study was HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-15a/15b (17%) followed by HPA-1a/1a-2a/2a-3a/3a-4a/4a-5a/5a-6a/6a-15a/15b (14.33%). The allele frequencies of HPA in Malays and Chinese were found to be similar those of other East and South-East Asian populations, while those of Indians were comparable to the frequencies found in Europeans. CONCLUSION: The results of this study have been useful for determining the distribution of HPA polymorphisms in this region and for potential clinical implications 2012 Article PeerReviewed Tan, J.Y. and Lian, L.H. and Nadarajan, V.S. (2012) Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population. Blood Transfusion , 4 (1-9). ISSN 1723-2007 http://www.ncbi.nlm.nih.gov/pubmed/22682339 doi: 10.2450/2012.0095-11
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Tan, J.Y.
Lian, L.H.
Nadarajan, V.S.
Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
description BACKGROUND: Human platelet antigens (HPA) are determinant in several platelet-specific alloimmune disorders, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet transfusion refractoriness. The distribution of HPA systems in the Malaysian population is not known. Defining the patterns of HPA systems provides a basis for risk assessment and management of the above complications. MATERIALS AND METHODS: The aim of this study was to investigate the distribution of HPA -1 to -6 and -15 in the three major ethnic groups (Malay, Chinese and Indian) in the Malaysian population. A total of 600 random donor samples, 200 from each of the three ethnic groups, were genotyped by means of real time polymerase chain reaction (PCR) with hydrolysis probes and PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS: The most common genotype observed in this study was HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-15a/15b (17%) followed by HPA-1a/1a-2a/2a-3a/3a-4a/4a-5a/5a-6a/6a-15a/15b (14.33%). The allele frequencies of HPA in Malays and Chinese were found to be similar those of other East and South-East Asian populations, while those of Indians were comparable to the frequencies found in Europeans. CONCLUSION: The results of this study have been useful for determining the distribution of HPA polymorphisms in this region and for potential clinical implications
format Article
author Tan, J.Y.
Lian, L.H.
Nadarajan, V.S.
author_facet Tan, J.Y.
Lian, L.H.
Nadarajan, V.S.
author_sort Tan, J.Y.
title Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
title_short Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
title_full Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
title_fullStr Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
title_full_unstemmed Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population
title_sort genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the malaysian population
publishDate 2012
url http://eprints.um.edu.my/3310/
http://www.ncbi.nlm.nih.gov/pubmed/22682339
_version_ 1643687087125299200
score 13.211869

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