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Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina

In this study, bacterial strains Ha5(T), Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplifi...

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Main Authors: Chua, Kah-Ooi, See-Too, Wah-Seng, Tan, Jia-Yi, Song, Sze-Looi, Yong, Hoi-Sen, Yin, Wai-Fong, Chan, Kok-Gan
Format: Article
Published: Microbiological Society Korea 2020
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QR Microbiology
Online Access:http://eprints.um.edu.my/31605/
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spelling my.um.eprints.316052022-10-20T03:02:51Z http://eprints.um.edu.my/31605/ Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina Chua, Kah-Ooi See-Too, Wah-Seng Tan, Jia-Yi Song, Sze-Looi Yong, Hoi-Sen Yin, Wai-Fong Chan, Kok-Gan QR Microbiology In this study, bacterial strains Ha5(T), Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15-37 degrees C (optimum, 28-30 degrees C) and in the presence of 0-1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C-18:1 omega 7c, C-16:0, C-19:0 omega 8c cyclo, C-14:0, and C-16:0 2-OH. Strains Ha5(T), Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56-94.63%) with Neokomagataea tanensis NBRC106556(T) of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5(T), Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5(T) as the type strain. Microbiological Society Korea 2020-12 Article PeerReviewed Chua, Kah-Ooi and See-Too, Wah-Seng and Tan, Jia-Yi and Song, Sze-Looi and Yong, Hoi-Sen and Yin, Wai-Fong and Chan, Kok-Gan (2020) Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina. Journal of Microbiology, 58 (12). pp. 988-997. ISSN 1225-8873, DOI https://doi.org/10.1007/s12275-020-0325-8 <https://doi.org/10.1007/s12275-020-0325-8>. 10.1007/s12275-020-0325-8
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QR Microbiology
spellingShingle QR Microbiology
Chua, Kah-Ooi
See-Too, Wah-Seng
Tan, Jia-Yi
Song, Sze-Looi
Yong, Hoi-Sen
Yin, Wai-Fong
Chan, Kok-Gan
Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
description In this study, bacterial strains Ha5(T), Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15-37 degrees C (optimum, 28-30 degrees C) and in the presence of 0-1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C-18:1 omega 7c, C-16:0, C-19:0 omega 8c cyclo, C-14:0, and C-16:0 2-OH. Strains Ha5(T), Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56-94.63%) with Neokomagataea tanensis NBRC106556(T) of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5(T), Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5(T) as the type strain.
format Article
author Chua, Kah-Ooi
See-Too, Wah-Seng
Tan, Jia-Yi
Song, Sze-Looi
Yong, Hoi-Sen
Yin, Wai-Fong
Chan, Kok-Gan
author_facet Chua, Kah-Ooi
See-Too, Wah-Seng
Tan, Jia-Yi
Song, Sze-Looi
Yong, Hoi-Sen
Yin, Wai-Fong
Chan, Kok-Gan
author_sort Chua, Kah-Ooi
title Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
title_short Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
title_full Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
title_fullStr Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
title_full_unstemmed Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
title_sort oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant oecophylla smaragdina
publisher Microbiological Society Korea
publishDate 2020
url http://eprints.um.edu.my/31605/
_version_ 1748181063081918464
score 13.211869

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