Staff View: In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)

The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell...

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Main Authors:	Isa, N.M., Abdelwahab, S.I., Mohan, S., Abdul, A.B., Sukari, M.A., Taha, M.M.E., Syam, S., Narrima, P., Cheah, S.C., Ahmad, S., Mustafa, Mohd Rais
Format:	Article
Language:	English
Published:	2012
Subjects:	Q Science (General) R Medicine
Online Access:	http://eprints.um.edu.my/3026/1/In_vitro_anti-inflammatory%2C_cytotoxic_and_antioxidant_activities_of_boesenbergin_A%2C_a_chalcone_isolated_from_Boesenbergia_rotunda_%28L.%29_%28fingerroot%29.pdf http://eprints.um.edu.my/3026/ http://www.scielo.br/scielo.php?pid=S0100-879X2012007500022&script=sci_arttext
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spelling	my.um.eprints.30262019-12-18T06:40:24Z http://eprints.um.edu.my/3026/ In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot) Isa, N.M. Abdelwahab, S.I. Mohan, S. Abdul, A.B. Sukari, M.A. Taha, M.M.E. Syam, S. Narrima, P. Cheah, S.C. Ahmad, S. Mustafa, Mohd Rais Q Science (General) R Medicine The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 Âµg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 Â± 3.15, 10.69 Â± 2.64, 20.31 Â± 1.34, 94.10 Â± 1.19, and 9.324 Â± 0.24 Âµg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 Âµg/mL) and quercetin (5 Âµg/mL) were equivalent to a Trolox concentration of 11.91 Â± 0.23 and 160.32 Â± 2.75 ÂµM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 ÂµM and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 ÂµM. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract. 2012 Article NonPeerReviewed application/pdf en http://eprints.um.edu.my/3026/1/In_vitro_anti-inflammatory%2C_cytotoxic_and_antioxidant_activities_of_boesenbergin_A%2C_a_chalcone_isolated_from_Boesenbergia_rotunda_%28L.%29_%28fingerroot%29.pdf Isa, N.M. and Abdelwahab, S.I. and Mohan, S. and Abdul, A.B. and Sukari, M.A. and Taha, M.M.E. and Syam, S. and Narrima, P. and Cheah, S.C. and Ahmad, S. and Mustafa, Mohd Rais (2012) In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot). Brazilian Journal of Medical and Biological Research (REVIEW). 0-0. ISSN 0100-879X http://www.scielo.br/scielo.php?pid=S0100-879X2012007500022&script=sci_arttext 10.1590/S0100-879X2012007500022
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
language	English
topic	Q Science (General) R Medicine
spellingShingle	Q Science (General) R Medicine Isa, N.M. Abdelwahab, S.I. Mohan, S. Abdul, A.B. Sukari, M.A. Taha, M.M.E. Syam, S. Narrima, P. Cheah, S.C. Ahmad, S. Mustafa, Mohd Rais In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
description	The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 Âµg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 Â± 3.15, 10.69 Â± 2.64, 20.31 Â± 1.34, 94.10 Â± 1.19, and 9.324 Â± 0.24 Âµg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 Âµg/mL) and quercetin (5 Âµg/mL) were equivalent to a Trolox concentration of 11.91 Â± 0.23 and 160.32 Â± 2.75 ÂµM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 ÂµM and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 ÂµM. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract.
format	Article
author	Isa, N.M. Abdelwahab, S.I. Mohan, S. Abdul, A.B. Sukari, M.A. Taha, M.M.E. Syam, S. Narrima, P. Cheah, S.C. Ahmad, S. Mustafa, Mohd Rais
author_facet	Isa, N.M. Abdelwahab, S.I. Mohan, S. Abdul, A.B. Sukari, M.A. Taha, M.M.E. Syam, S. Narrima, P. Cheah, S.C. Ahmad, S. Mustafa, Mohd Rais
author_sort	Isa, N.M.
title	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
title_short	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
title_full	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
title_fullStr	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
title_full_unstemmed	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)
title_sort	in vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin a, a chalcone isolated from boesenbergia rotunda (l.)(fingerroot)
publishDate	2012
url	http://eprints.um.edu.my/3026/1/In_vitro_anti-inflammatory%2C_cytotoxic_and_antioxidant_activities_of_boesenbergin_A%2C_a_chalcone_isolated_from_Boesenbergia_rotunda_%28L.%29_%28fingerroot%29.pdf http://eprints.um.edu.my/3026/ http://www.scielo.br/scielo.php?pid=S0100-879X2012007500022&script=sci_arttext
_version_	1654960588260900864
score	13.211869

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.)(fingerroot)

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