Staff View: Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

Background: Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods: Streptomyces strains' gr...

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Main Authors:	Ahmad, Siti Junaidah, Zin, Noraziah Mohamad, Mazlan, Noor Wini, Baharum, Syarul Nataqain, Baba, Mohd Shukri, Lau, Yee Ling
Format:	Article
Published:	PeerJ 2021
Subjects:	R Medicine (General) RB Pathology
Online Access:	http://eprints.um.edu.my/28430/
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spelling	my.um.eprints.284302022-08-08T06:09:41Z http://eprints.um.edu.my/28430/ Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential Ahmad, Siti Junaidah Zin, Noraziah Mohamad Mazlan, Noor Wini Baharum, Syarul Nataqain Baba, Mohd Shukri Lau, Yee Ling R Medicine (General) RB Pathology Background: Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods: Streptomyces strains' growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC50 values were calculated. Metabolomics analysis was conducted on both strains' mid-exponential and stationary phase extracts. Results: The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC50 values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC50 value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done. PeerJ 2021-03-15 Article PeerReviewed Ahmad, Siti Junaidah and Zin, Noraziah Mohamad and Mazlan, Noor Wini and Baharum, Syarul Nataqain and Baba, Mohd Shukri and Lau, Yee Ling (2021) Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential. PeerJ, 9. ISSN 2167-8359, DOI https://doi.org/10.7717/peerj.10816 <https://doi.org/10.7717/peerj.10816>. 10.7717/peerj.10816
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General) RB Pathology
spellingShingle	R Medicine (General) RB Pathology Ahmad, Siti Junaidah Zin, Noraziah Mohamad Mazlan, Noor Wini Baharum, Syarul Nataqain Baba, Mohd Shukri Lau, Yee Ling Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
description	Background: Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods: Streptomyces strains' growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC50 values were calculated. Metabolomics analysis was conducted on both strains' mid-exponential and stationary phase extracts. Results: The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC50 values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC50 value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done.
format	Article
author	Ahmad, Siti Junaidah Zin, Noraziah Mohamad Mazlan, Noor Wini Baharum, Syarul Nataqain Baba, Mohd Shukri Lau, Yee Ling
author_facet	Ahmad, Siti Junaidah Zin, Noraziah Mohamad Mazlan, Noor Wini Baharum, Syarul Nataqain Baba, Mohd Shukri Lau, Yee Ling
author_sort	Ahmad, Siti Junaidah
title	Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
title_short	Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
title_full	Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
title_fullStr	Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
title_full_unstemmed	Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
title_sort	metabolite profiling of endophytic streptomyces spp. and its antiplasmodial potential
publisher	PeerJ
publishDate	2021
url	http://eprints.um.edu.my/28430/
_version_	1740826013852499968
score	13.211869

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

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