Cover Image

Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis

Background: In neurological diseases, neuronal loss is frequently associated with overproduction of free radicals and reduced level of endogenous neurotrophic factors. The blue-green microalga, Spirulina platensis is a well-known superfood with a high content of diverse nutrients and possesses sever...

Full description

Saved in:
Bibliographic Details
Main Authors: Ngu, Ee-Ling, Ko, Chen-Lin, Tan, Cheng-Yau, Wong, Kah-Hui, Phang, Siew-Moi, Yow, Yoon-Yen
Format: Article
Published: Association of Pharmaceutical Teachers of India 2021
Subjects:
R Medicine (General)
Online Access:http://eprints.um.edu.my/28395/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.28395
record_format eprints
spelling my.um.eprints.283952022-08-03T03:50:36Z http://eprints.um.edu.my/28395/ Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis Ngu, Ee-Ling Ko, Chen-Lin Tan, Cheng-Yau Wong, Kah-Hui Phang, Siew-Moi Yow, Yoon-Yen R Medicine (General) Background: In neurological diseases, neuronal loss is frequently associated with overproduction of free radicals and reduced level of endogenous neurotrophic factors. The blue-green microalga, Spirulina platensis is a well-known superfood with a high content of diverse nutrients and possesses several therapeutic properties. Here, we aimed to study the neuritogenic and antioxidant activities of Spirulina platensis UMACC 159. Materials and Methods: PC-12Adh (rat pheochromocytoma) cell was used to investigate the cytotoxicity effect of S. platensis UMACC 159 extracts (water, methanol, and ethanol) via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Neuritogenic activity of the extracts towards PC-12Adh cell line was studied using neurite outgrowth assay and immunofluorescence imaging of neurofilaments. The extracts were screened for the phytochemical contents, and antioxidant activities using 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-Diphenyl-1-pircrylhydrazyl (DPPH) and reducing power. Results: Ethanol extract was found to exhibit the highest neuritogenic effect and enhanced the cytoskeleton formation in PC-12Adh cells at 6.25 mu g/mL. Ethanol extract also showed the highest total phenolic content (49.09 +/- 1.35 mg GAE/g), ABTS (EC50 of 1.34 +/- 0.01 mg/mL) and DPPH (EC50 of 0.45 +/- 0.04 mg/mL) scavenging activities (P <= 0.05), suggesting that the neuritogenic effect of ethanol extract was attributed to the phenolic compound(s) via antioxidant activity. Conclusion: Ethanol extract contains bioactive compound(s) with similar neuritogenic activity as nerve growth factor for neuronal survival, growth, and axonal regeneration. S. platensis has been proposed as a promising cognitive supplement. Association of Pharmaceutical Teachers of India 2021-07 Article PeerReviewed Ngu, Ee-Ling and Ko, Chen-Lin and Tan, Cheng-Yau and Wong, Kah-Hui and Phang, Siew-Moi and Yow, Yoon-Yen (2021) Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis. Indian Journal of Pharmaceutical Education and Research, 55 (3). pp. 812-822. ISSN 0019-5464, DOI https://doi.org/10.5530/ijper.55.3.154 <https://doi.org/10.5530/ijper.55.3.154>. 10.5530/ijper.55.3.154
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Ngu, Ee-Ling
Ko, Chen-Lin
Tan, Cheng-Yau
Wong, Kah-Hui
Phang, Siew-Moi
Yow, Yoon-Yen
Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
description Background: In neurological diseases, neuronal loss is frequently associated with overproduction of free radicals and reduced level of endogenous neurotrophic factors. The blue-green microalga, Spirulina platensis is a well-known superfood with a high content of diverse nutrients and possesses several therapeutic properties. Here, we aimed to study the neuritogenic and antioxidant activities of Spirulina platensis UMACC 159. Materials and Methods: PC-12Adh (rat pheochromocytoma) cell was used to investigate the cytotoxicity effect of S. platensis UMACC 159 extracts (water, methanol, and ethanol) via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Neuritogenic activity of the extracts towards PC-12Adh cell line was studied using neurite outgrowth assay and immunofluorescence imaging of neurofilaments. The extracts were screened for the phytochemical contents, and antioxidant activities using 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-Diphenyl-1-pircrylhydrazyl (DPPH) and reducing power. Results: Ethanol extract was found to exhibit the highest neuritogenic effect and enhanced the cytoskeleton formation in PC-12Adh cells at 6.25 mu g/mL. Ethanol extract also showed the highest total phenolic content (49.09 +/- 1.35 mg GAE/g), ABTS (EC50 of 1.34 +/- 0.01 mg/mL) and DPPH (EC50 of 0.45 +/- 0.04 mg/mL) scavenging activities (P <= 0.05), suggesting that the neuritogenic effect of ethanol extract was attributed to the phenolic compound(s) via antioxidant activity. Conclusion: Ethanol extract contains bioactive compound(s) with similar neuritogenic activity as nerve growth factor for neuronal survival, growth, and axonal regeneration. S. platensis has been proposed as a promising cognitive supplement.
format Article
author Ngu, Ee-Ling
Ko, Chen-Lin
Tan, Cheng-Yau
Wong, Kah-Hui
Phang, Siew-Moi
Yow, Yoon-Yen
author_facet Ngu, Ee-Ling
Ko, Chen-Lin
Tan, Cheng-Yau
Wong, Kah-Hui
Phang, Siew-Moi
Yow, Yoon-Yen
author_sort Ngu, Ee-Ling
title Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
title_short Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
title_full Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
title_fullStr Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
title_full_unstemmed Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
title_sort phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis
publisher Association of Pharmaceutical Teachers of India
publishDate 2021
url http://eprints.um.edu.my/28395/
_version_ 1740826009274417152
score 13.211869

Similar Items