Staff View: DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations

DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations

Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity,...

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Main Authors:	Khalil, Ibrahim, Hashem, Abu, Nath, Amit R., Julkapli, Nurhidayatullaili Muhd, Yehye, Wageeh A., Basirun, Wan Jeffrey
Format:	Article
Published:	2021
Subjects:	QH426 Genetics Medical technology TA Engineering (General). Civil engineering (General)
Online Access:	http://eprints.um.edu.my/28280/
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spelling	my.um.eprints.282802022-03-29T08:07:15Z http://eprints.um.edu.my/28280/ DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations Khalil, Ibrahim Hashem, Abu Nath, Amit R. Julkapli, Nurhidayatullaili Muhd Yehye, Wageeh A. Basirun, Wan Jeffrey QH426 Genetics Medical technology TA Engineering (General). Civil engineering (General) Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity, sensitivity and unsuitability for processed food products or complex food matrices. On the other hand, DNA based molecular techniques and nanoparticle based DNA biosensing strategies are gathering huge attention from the scientific communities, researchers and are considered as one of the best alternatives to the conventional strategies. Though nucleic acid based molecular techniques such as PCR and DNA sequencing are getting greater successes in species detection, they are still facing problems from its point-of-care applications. In this context, nanoparticle based DNA biosensors have gathered successes in some extent but not to a satisfactory stage to mark with. In recent years, many articles have been published in the area of progressive nucleic acid-based technologies, however there are very few review articles on DNA nanobiosensors in food science and technology. In this review, we present the fundamentals of DNA based molecular techniques such as PCR, DNA sequencing and their applications in food science. Moreover, the in-depth discussions of different DNA biosensing strategies or more specifically electrochemical and optical DNA nanobiosensors are presented. In addition, the significance of DNA nanobiosensors over other advanced detection technologies is discussed, focusing on the deficiencies, advantages as well as current challenges to ameliorate with the direction for future development. 2021 Article PeerReviewed Khalil, Ibrahim and Hashem, Abu and Nath, Amit R. and Julkapli, Nurhidayatullaili Muhd and Yehye, Wageeh A. and Basirun, Wan Jeffrey (2021) DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations. Molecular and Cellular Probes, 59. ISSN 0890-8508, DOI https://doi.org/10.1016/j.mcp.2021.101758 <https://doi.org/10.1016/j.mcp.2021.101758>. 10.1016/j.mcp.2021.101758
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QH426 Genetics Medical technology TA Engineering (General). Civil engineering (General)
spellingShingle	QH426 Genetics Medical technology TA Engineering (General). Civil engineering (General) Khalil, Ibrahim Hashem, Abu Nath, Amit R. Julkapli, Nurhidayatullaili Muhd Yehye, Wageeh A. Basirun, Wan Jeffrey DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
description	Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity, sensitivity and unsuitability for processed food products or complex food matrices. On the other hand, DNA based molecular techniques and nanoparticle based DNA biosensing strategies are gathering huge attention from the scientific communities, researchers and are considered as one of the best alternatives to the conventional strategies. Though nucleic acid based molecular techniques such as PCR and DNA sequencing are getting greater successes in species detection, they are still facing problems from its point-of-care applications. In this context, nanoparticle based DNA biosensors have gathered successes in some extent but not to a satisfactory stage to mark with. In recent years, many articles have been published in the area of progressive nucleic acid-based technologies, however there are very few review articles on DNA nanobiosensors in food science and technology. In this review, we present the fundamentals of DNA based molecular techniques such as PCR, DNA sequencing and their applications in food science. Moreover, the in-depth discussions of different DNA biosensing strategies or more specifically electrochemical and optical DNA nanobiosensors are presented. In addition, the significance of DNA nanobiosensors over other advanced detection technologies is discussed, focusing on the deficiencies, advantages as well as current challenges to ameliorate with the direction for future development.
format	Article
author	Khalil, Ibrahim Hashem, Abu Nath, Amit R. Julkapli, Nurhidayatullaili Muhd Yehye, Wageeh A. Basirun, Wan Jeffrey
author_facet	Khalil, Ibrahim Hashem, Abu Nath, Amit R. Julkapli, Nurhidayatullaili Muhd Yehye, Wageeh A. Basirun, Wan Jeffrey
author_sort	Khalil, Ibrahim
title	DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
title_short	DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
title_full	DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
title_fullStr	DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
title_full_unstemmed	DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations
title_sort	dna/nano based advanced genetic detection tools for authentication of species: strategies, prospects and limitations
publishDate	2021
url	http://eprints.um.edu.my/28280/
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score	13.211869

DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations

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