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Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways

This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed w...

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Main Authors: Vendidandala, Nagarjuna Reddy, Yin, Tan Pei, Nelli, Giribabu, Pasupuleti, Visweswara Rao, Nyamathulla, Shaik, Mokhtar, Seri Intan
Format: Article
Published: Elsevier 2021
Subjects:
R Medicine (General)
RM Therapeutics. Pharmacology
Online Access:http://eprints.um.edu.my/28270/
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spelling my.um.eprints.282702022-07-29T07:07:35Z http://eprints.um.edu.my/28270/ Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways Vendidandala, Nagarjuna Reddy Yin, Tan Pei Nelli, Giribabu Pasupuleti, Visweswara Rao Nyamathulla, Shaik Mokhtar, Seri Intan R Medicine (General) RM Therapeutics. Pharmacology This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed with blank CGP1; 3. Diabetic rats dressed with 13.06 mu M of GC; 4. Diabetic rats dressed with 26.12 mu M of GC; 5. Diabetic rats dressed with 0.1% silver sulfadiazine patches. GC-AgNPs-CGP dressed diabetic rats showed significant FBG reduction, prevented the body weight losses and reduced the oxidative stress by lowering MDA content and elevated antioxidant enzymes such as SOD, CAT and GPx in wound healing skin of diabetic rats when compared to normal CGP. Besides, mRNA expression of Nrf2, Nqo-1, and Ho-1 was upregulated with downregulated expression of Keap-1 mRNA, which is supported by immunohistochemistry. Furthermore, GC-AgNPs-CGP dressing increased growth factors such as VEGF, EGF, TGF-beta, and FGF-2 while decreasing MMP-2 in the skin of diabetic wound rats. In vitro permeation study demonstrated rapid GC release and permeation with a flux of 0.061 and 0.143 mg/sq.cm/h. In conclusion, the results indicated that GC-AgNPs-CGP dressing on diabetic wound rats modulated oxidative stress and inflammation with elevated growth factors; increased collagen synthesis thereby significantly improved the wound healing and could be beneficial for the management of diabetic wounds. Elsevier 2021-12-01 Article PeerReviewed Vendidandala, Nagarjuna Reddy and Yin, Tan Pei and Nelli, Giribabu and Pasupuleti, Visweswara Rao and Nyamathulla, Shaik and Mokhtar, Seri Intan (2021) Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways. Life Sciences, 286. ISSN 0024-3205, DOI https://doi.org/10.1016/j.lfs.2021.120019 <https://doi.org/10.1016/j.lfs.2021.120019>. 10.1016/j.lfs.2021.120019
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
RM Therapeutics. Pharmacology
spellingShingle R Medicine (General)
RM Therapeutics. Pharmacology
Vendidandala, Nagarjuna Reddy
Yin, Tan Pei
Nelli, Giribabu
Pasupuleti, Visweswara Rao
Nyamathulla, Shaik
Mokhtar, Seri Intan
Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
description This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed with blank CGP1; 3. Diabetic rats dressed with 13.06 mu M of GC; 4. Diabetic rats dressed with 26.12 mu M of GC; 5. Diabetic rats dressed with 0.1% silver sulfadiazine patches. GC-AgNPs-CGP dressed diabetic rats showed significant FBG reduction, prevented the body weight losses and reduced the oxidative stress by lowering MDA content and elevated antioxidant enzymes such as SOD, CAT and GPx in wound healing skin of diabetic rats when compared to normal CGP. Besides, mRNA expression of Nrf2, Nqo-1, and Ho-1 was upregulated with downregulated expression of Keap-1 mRNA, which is supported by immunohistochemistry. Furthermore, GC-AgNPs-CGP dressing increased growth factors such as VEGF, EGF, TGF-beta, and FGF-2 while decreasing MMP-2 in the skin of diabetic wound rats. In vitro permeation study demonstrated rapid GC release and permeation with a flux of 0.061 and 0.143 mg/sq.cm/h. In conclusion, the results indicated that GC-AgNPs-CGP dressing on diabetic wound rats modulated oxidative stress and inflammation with elevated growth factors; increased collagen synthesis thereby significantly improved the wound healing and could be beneficial for the management of diabetic wounds.
format Article
author Vendidandala, Nagarjuna Reddy
Yin, Tan Pei
Nelli, Giribabu
Pasupuleti, Visweswara Rao
Nyamathulla, Shaik
Mokhtar, Seri Intan
author_facet Vendidandala, Nagarjuna Reddy
Yin, Tan Pei
Nelli, Giribabu
Pasupuleti, Visweswara Rao
Nyamathulla, Shaik
Mokhtar, Seri Intan
author_sort Vendidandala, Nagarjuna Reddy
title Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
title_short Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
title_full Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
title_fullStr Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
title_full_unstemmed Gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways
title_sort gallocatechin-silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the nrf2/ho-1 and tlr4/nf-kappa b pathways
publisher Elsevier
publishDate 2021
url http://eprints.um.edu.my/28270/
_version_ 1740826000642539520
score 13.211869

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