Cover Image

Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis

Research background. Ophiocordyceps sinensis, a highly valued medicinal fungus, is close to extinction due to overexploitation. Successful cultivation of O. sinensis fruiting body (OCS02 (R)) shows that the cultivar has a promising nutritional value and numerous bioactive compounds. Antioxidant and...

Full description

Saved in:
Bibliographic Details
Main Authors: Kong, Boon-Hong, Yap, Chee-Sum Alvin, Mohamad Razif, Muhammad Fazril, Ng, Szu-Ting, Tan, Chon-Seng, Fung, Shin-Yee
Format: Article
Published: Faculty Food Technology Biotechnology 2021
Subjects:
Q Science (General)
Online Access:http://eprints.um.edu.my/28223/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.28223
record_format eprints
spelling my.um.eprints.282232022-03-05T02:57:02Z http://eprints.um.edu.my/28223/ Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis Kong, Boon-Hong Yap, Chee-Sum Alvin Mohamad Razif, Muhammad Fazril Ng, Szu-Ting Tan, Chon-Seng Fung, Shin-Yee Q Science (General) Research background. Ophiocordyceps sinensis, a highly valued medicinal fungus, is close to extinction due to overexploitation. Successful cultivation of O. sinensis fruiting body (OCS02 (R)) shows that the cultivar has a promising nutritional value and numerous bioactive compounds. Antioxidant and antiproliferative properties and biologically active proteins of the OCS02 (R) are investigated for possible development into nutraceuticals. Experimental approach. The chemical composition of the OCS02 (R) cold water extract was determined, and the antioxidant activities were examined using ferric reducing, DPPH. and O2. - scavenging assays. Tetrazolium dye (MTT) cytotoxic assay was performed to assess the antiproliferative activity of the extract. Bioactive proteins in the active fraction of the extract were identified using liquid chromatography (LC) and tandem-mass spectrometry (MS/MS). Results and conclusions. The OCS02 (R) extract exhibited strong O-2(center dot)-scavenging (expressed as Trolox equivalents (18.4 +/- 1.1) mol/g) and potent cytotoxic activities against adenocarcinomic human alveolar basal epithelial (A549) cells (IC50=(58.2 +/- 6.8) mu g/mL). High molecular mass polysaccharides, proteins and protein-polysaccharide complexes could have contributed to the antioxidant and cytotoxic selectivity of the OCS02 (R). LC-MS/MS analysis identified several potential cytotoxic proteases and an oxalate decarboxylase protein which may exhibit protection effects on kidneys. Novelty and scientific contributions. The findings demonstrate the potential of OCS02 (R) to be developed into functional food due to its promising superoxide anion radical scavenging capacity, cytotoxic effect and presence of biopharmaceutically active proteins. Faculty Food Technology Biotechnology 2021-06 Article PeerReviewed Kong, Boon-Hong and Yap, Chee-Sum Alvin and Mohamad Razif, Muhammad Fazril and Ng, Szu-Ting and Tan, Chon-Seng and Fung, Shin-Yee (2021) Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis. Food Technology and Biotechnology, 59 (2). pp. 201-208. ISSN 1330-9862, DOI https://doi.org/10.17113/ftb.59.02.21.7151 <https://doi.org/10.17113/ftb.59.02.21.7151>. 10.17113/ftb.59.02.21.7151
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Kong, Boon-Hong
Yap, Chee-Sum Alvin
Mohamad Razif, Muhammad Fazril
Ng, Szu-Ting
Tan, Chon-Seng
Fung, Shin-Yee
Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
description Research background. Ophiocordyceps sinensis, a highly valued medicinal fungus, is close to extinction due to overexploitation. Successful cultivation of O. sinensis fruiting body (OCS02 (R)) shows that the cultivar has a promising nutritional value and numerous bioactive compounds. Antioxidant and antiproliferative properties and biologically active proteins of the OCS02 (R) are investigated for possible development into nutraceuticals. Experimental approach. The chemical composition of the OCS02 (R) cold water extract was determined, and the antioxidant activities were examined using ferric reducing, DPPH. and O2. - scavenging assays. Tetrazolium dye (MTT) cytotoxic assay was performed to assess the antiproliferative activity of the extract. Bioactive proteins in the active fraction of the extract were identified using liquid chromatography (LC) and tandem-mass spectrometry (MS/MS). Results and conclusions. The OCS02 (R) extract exhibited strong O-2(center dot)-scavenging (expressed as Trolox equivalents (18.4 +/- 1.1) mol/g) and potent cytotoxic activities against adenocarcinomic human alveolar basal epithelial (A549) cells (IC50=(58.2 +/- 6.8) mu g/mL). High molecular mass polysaccharides, proteins and protein-polysaccharide complexes could have contributed to the antioxidant and cytotoxic selectivity of the OCS02 (R). LC-MS/MS analysis identified several potential cytotoxic proteases and an oxalate decarboxylase protein which may exhibit protection effects on kidneys. Novelty and scientific contributions. The findings demonstrate the potential of OCS02 (R) to be developed into functional food due to its promising superoxide anion radical scavenging capacity, cytotoxic effect and presence of biopharmaceutically active proteins.
format Article
author Kong, Boon-Hong
Yap, Chee-Sum Alvin
Mohamad Razif, Muhammad Fazril
Ng, Szu-Ting
Tan, Chon-Seng
Fung, Shin-Yee
author_facet Kong, Boon-Hong
Yap, Chee-Sum Alvin
Mohamad Razif, Muhammad Fazril
Ng, Szu-Ting
Tan, Chon-Seng
Fung, Shin-Yee
author_sort Kong, Boon-Hong
title Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
title_short Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
title_full Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
title_fullStr Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
title_full_unstemmed Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis
title_sort antioxidant and cytotoxic effects and identification of ophiocordyceps sinensis bioactive proteins using shotgun proteomic analysis
publisher Faculty Food Technology Biotechnology
publishDate 2021
url http://eprints.um.edu.my/28223/
_version_ 1735409545170124800
score 13.211869

Similar Items