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Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope

Chikungunya virus (CHIKV) infection is the cause of acute symptoms and chronic symmetrical polyarthritis associated with long-term morbidity and mortality. Currently, there is no available licensed vaccine or particularly useful drug for human use against CHIKV infection. This study was conducted to...

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Main Authors: Rashid, Nor N., Teoh, Teow Chong, Al-Harbi, S. J., Yusof, R., Rothan, H. A.
Format: Article
Published: 2021
Subjects:
QL Zoology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:http://eprints.um.edu.my/27898/
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spelling my.um.eprints.278982022-04-07T06:11:11Z http://eprints.um.edu.my/27898/ Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope Rashid, Nor N. Teoh, Teow Chong Al-Harbi, S. J. Yusof, R. Rothan, H. A. QL Zoology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Chikungunya virus (CHIKV) infection is the cause of acute symptoms and chronic symmetrical polyarthritis associated with long-term morbidity and mortality. Currently, there is no available licensed vaccine or particularly useful drug for human use against CHIKV infection. This study was conducted to evaluate the efficacy of antibodies produced by papaya mosaic virus (PapMV) nanoparticles fused to E2EP3 peptide of CHIKV envelope as a recombinant CHIKV vaccine. PapMV, PapMV-C-E2EP3, and E2EP3-N-PapMV were produced in E. coli with an approximate size of 27 to 30 kDa. ICR mice (5 to 6 weeks of age) were injected subcutaneously with 25 micrograms of vaccine construct, and ELISA measured the titer of CHIKV specific IgG antibodies. The results showed that both recombinant proteins E2EP3-N-PapMV and PapMV-C-E2EP3 were able to induce IgG antibodies production in immunized mice against CHIKV while immunization with recombinant PapMV showed no IgG antibodies induction. The neutralizing activity of the antibodies generated by either E2EP3-N-PapMV or PapMV-C-E2EP3 exhibited similar inhibition to CHIKV replication in Vero cells using the cells based antibody neutralizing assay and analyzed by plaque formation assay. This study showed the effectiveness of nanoparticles vaccine generated by fusing epitope peptide of CHIKV envelope to papaya mosaic virus envelope in inducing a robust immune response in mice against CHIKV. The data showed that levels of neutralizing antibodies correlate with a protective immune response CHIKV replication. 2021-03 Article PeerReviewed Rashid, Nor N. and Teoh, Teow Chong and Al-Harbi, S. J. and Yusof, R. and Rothan, H. A. (2021) Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope. Tropical Biomedicine, 38 (1). pp. 36-41. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.38.1.007 <https://doi.org/10.47665/tb.38.1.007>. 10.47665/tb.38.1.007
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QL Zoology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle QL Zoology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Rashid, Nor N.
Teoh, Teow Chong
Al-Harbi, S. J.
Yusof, R.
Rothan, H. A.
Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
description Chikungunya virus (CHIKV) infection is the cause of acute symptoms and chronic symmetrical polyarthritis associated with long-term morbidity and mortality. Currently, there is no available licensed vaccine or particularly useful drug for human use against CHIKV infection. This study was conducted to evaluate the efficacy of antibodies produced by papaya mosaic virus (PapMV) nanoparticles fused to E2EP3 peptide of CHIKV envelope as a recombinant CHIKV vaccine. PapMV, PapMV-C-E2EP3, and E2EP3-N-PapMV were produced in E. coli with an approximate size of 27 to 30 kDa. ICR mice (5 to 6 weeks of age) were injected subcutaneously with 25 micrograms of vaccine construct, and ELISA measured the titer of CHIKV specific IgG antibodies. The results showed that both recombinant proteins E2EP3-N-PapMV and PapMV-C-E2EP3 were able to induce IgG antibodies production in immunized mice against CHIKV while immunization with recombinant PapMV showed no IgG antibodies induction. The neutralizing activity of the antibodies generated by either E2EP3-N-PapMV or PapMV-C-E2EP3 exhibited similar inhibition to CHIKV replication in Vero cells using the cells based antibody neutralizing assay and analyzed by plaque formation assay. This study showed the effectiveness of nanoparticles vaccine generated by fusing epitope peptide of CHIKV envelope to papaya mosaic virus envelope in inducing a robust immune response in mice against CHIKV. The data showed that levels of neutralizing antibodies correlate with a protective immune response CHIKV replication.
format Article
author Rashid, Nor N.
Teoh, Teow Chong
Al-Harbi, S. J.
Yusof, R.
Rothan, H. A.
author_facet Rashid, Nor N.
Teoh, Teow Chong
Al-Harbi, S. J.
Yusof, R.
Rothan, H. A.
author_sort Rashid, Nor N.
title Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
title_short Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
title_full Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
title_fullStr Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
title_full_unstemmed Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope
title_sort evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the e2ep3 peptide epitope of chikungunya envelope
publishDate 2021
url http://eprints.um.edu.my/27898/
_version_ 1735409536767885312
score 13.211869

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