Staff View: Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates

Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates

The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and inva...

Full description

Saved in:

Bibliographic Details
Main Authors:	Goh, Xiang Ting, Lim, Yvonne Ai Lian, Lee, Ping Chin, Nissapatorn, Veeranoot, Chua, Kek Heng
Format:	Article
Published:	ELSEVIER 2021
Subjects:	QD Chemistry QH301 Biology QL Zoology RA Public aspects of medicine
Online Access:	http://eprints.um.edu.my/27883/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.27883
record_format	eprints
spelling	my.um.eprints.278832022-03-31T04:50:58Z http://eprints.um.edu.my/27883/ Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates Goh, Xiang Ting Lim, Yvonne Ai Lian Lee, Ping Chin Nissapatorn, Veeranoot Chua, Kek Heng QD Chemistry QH301 Biology QL Zoology RA Public aspects of medicine The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and invasion of erythrocytes and it is considered one of the leading candidates for asexual blood stage vaccines. However, the genetic data of MSP-119 among human malaria parasites in Malaysia is limited and there is also a need to update the current sequence diversity of this gene region among the Thailand isolates. In this study, genomic DNA was extracted from 384 microscopypositive blood samples collected from patients who attended the hospitals or clinics in Malaysia and malaria clinics in Thailand from the year 2008 to 2016. The MSP-119 was amplified using PCR followed by bidirectional sequencing. DNA sequences identified in the present study were subjected to Median-joining network analysis with sequences of MSP-119 obtained from GenBank. DNA sequence analysis revealed that PfMSP-119 of Malaysian and Thailand isolates was not genetically conserved as high number of haplotypes were detected and positive selection was prevalent in PfMSP-119, hence questioning its suitability to be used as a vaccine candidate. A novel haplotype (Q/TNG/L) was also detected in Thailand P. falciparum isolate. In contrast, PvMSP-119 was highly conserved, however for the first time, a non-synonymous substitution (A1657S) was reported among Malaysian isolates. As for PkMSP-119, the presence of purifying selection and low nucleotide diversity indicated that it might be a potential vaccine target for P. knowlesi. ELSEVIER 2021-07 Article PeerReviewed Goh, Xiang Ting and Lim, Yvonne Ai Lian and Lee, Ping Chin and Nissapatorn, Veeranoot and Chua, Kek Heng (2021) Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates. Molecular and Biochemical Parasitology, 244. ISSN 0166-6851, DOI https://doi.org/10.1016/j.molbiopara.2021.111390 <https://doi.org/10.1016/j.molbiopara.2021.111390>. 10.1016/j.molbiopara.2021.111390
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QD Chemistry QH301 Biology QL Zoology RA Public aspects of medicine
spellingShingle	QD Chemistry QH301 Biology QL Zoology RA Public aspects of medicine Goh, Xiang Ting Lim, Yvonne Ai Lian Lee, Ping Chin Nissapatorn, Veeranoot Chua, Kek Heng Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
description	The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and invasion of erythrocytes and it is considered one of the leading candidates for asexual blood stage vaccines. However, the genetic data of MSP-119 among human malaria parasites in Malaysia is limited and there is also a need to update the current sequence diversity of this gene region among the Thailand isolates. In this study, genomic DNA was extracted from 384 microscopypositive blood samples collected from patients who attended the hospitals or clinics in Malaysia and malaria clinics in Thailand from the year 2008 to 2016. The MSP-119 was amplified using PCR followed by bidirectional sequencing. DNA sequences identified in the present study were subjected to Median-joining network analysis with sequences of MSP-119 obtained from GenBank. DNA sequence analysis revealed that PfMSP-119 of Malaysian and Thailand isolates was not genetically conserved as high number of haplotypes were detected and positive selection was prevalent in PfMSP-119, hence questioning its suitability to be used as a vaccine candidate. A novel haplotype (Q/TNG/L) was also detected in Thailand P. falciparum isolate. In contrast, PvMSP-119 was highly conserved, however for the first time, a non-synonymous substitution (A1657S) was reported among Malaysian isolates. As for PkMSP-119, the presence of purifying selection and low nucleotide diversity indicated that it might be a potential vaccine target for P. knowlesi.
format	Article
author	Goh, Xiang Ting Lim, Yvonne Ai Lian Lee, Ping Chin Nissapatorn, Veeranoot Chua, Kek Heng
author_facet	Goh, Xiang Ting Lim, Yvonne Ai Lian Lee, Ping Chin Nissapatorn, Veeranoot Chua, Kek Heng
author_sort	Goh, Xiang Ting
title	Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_short	Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_full	Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_fullStr	Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_full_unstemmed	Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_sort	diversity and natural selection of merozoite surface protein-1 in three species of human malaria parasites: contribution from south-east asian isolates
publisher	ELSEVIER
publishDate	2021
url	http://eprints.um.edu.my/27883/
_version_	1735409534727356416
score	13.211869

Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates

Similar Items