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Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery...

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Main Authors: Akinsola, Rasaq Olajide, Lee, Choon Weng, Sim, Edmund Ui Hang, Narayanan, Kumaran
Format: Article
Published: Academic Press Inc Elsevier Science 2021
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/26536/
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spelling my.um.eprints.265362022-03-15T08:21:37Z http://eprints.um.edu.my/26536/ Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency Akinsola, Rasaq Olajide Lee, Choon Weng Sim, Edmund Ui Hang Narayanan, Kumaran QH301 Biology Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency. Academic Press Inc Elsevier Science 2021-03-01 Article PeerReviewed Akinsola, Rasaq Olajide and Lee, Choon Weng and Sim, Edmund Ui Hang and Narayanan, Kumaran (2021) Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency. Analytical Biochemistry, 616. ISSN 0003-2697, DOI https://doi.org/10.1016/j.ab.2020.114088 <https://doi.org/10.1016/j.ab.2020.114088>. 10.1016/j.ab.2020.114088
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Akinsola, Rasaq Olajide
Lee, Choon Weng
Sim, Edmund Ui Hang
Narayanan, Kumaran
Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
description Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.
format Article
author Akinsola, Rasaq Olajide
Lee, Choon Weng
Sim, Edmund Ui Hang
Narayanan, Kumaran
author_facet Akinsola, Rasaq Olajide
Lee, Choon Weng
Sim, Edmund Ui Hang
Narayanan, Kumaran
author_sort Akinsola, Rasaq Olajide
title Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
title_short Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
title_full Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
title_fullStr Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
title_full_unstemmed Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
title_sort inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances e. coli bactofection efficiency
publisher Academic Press Inc Elsevier Science
publishDate 2021
url http://eprints.um.edu.my/26536/
_version_ 1735409425398628352
score 13.211869

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