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Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes

The cobalt(II), copper(II) and zinc(II) complexes of 1,10-phenanthroline (phen) and maltol (mal) (complexes 1, 2, 3 respectively) were prepared from their respective metal(II) chlorides and were characterized by FT-IR, elemental analysis, UV spectroscopy, molar conductivity, p-nitrosodimethylaniline...

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Main Authors: Ng, Chew Hee, Tan, Thean Heng, Tioh, Ngee Heng, Seng, Hoi Ling, Ahmad, Munirah, Ng, Seik Weng, Gan, Wei Khang, Low, May Lee, Lai, Jing Wei, Zulkefeli, Mohd
Format: Article
Published: 2021
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://eprints.um.edu.my/26211/
https://doi.org/10.1016/j.jinorgbio.2021.111453
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spelling my.um.eprints.262112022-02-18T01:35:50Z http://eprints.um.edu.my/26211/ Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes Ng, Chew Hee Tan, Thean Heng Tioh, Ngee Heng Seng, Hoi Ling Ahmad, Munirah Ng, Seik Weng Gan, Wei Khang Low, May Lee Lai, Jing Wei Zulkefeli, Mohd Q Science (General) QD Chemistry The cobalt(II), copper(II) and zinc(II) complexes of 1,10-phenanthroline (phen) and maltol (mal) (complexes 1, 2, 3 respectively) were prepared from their respective metal(II) chlorides and were characterized by FT-IR, elemental analysis, UV spectroscopy, molar conductivity, p-nitrosodimethylaniline assay and mass spectrometry. The X-ray structure of a single crystal of the zinc(II) analogue reveals a square pyramidal structure with distinctly shorter apical chloride bond. All complexes were evaluated for their anticancer property on breast cancer cell lines MCF-7 and MDA-MB-231, and normal cell line MCF-10A, using (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide assay and morphological studies. Complex 2 was most potent for 24, 48 and 72 h treatment of cancer cells but it was not selective towards cancer over normal cells. The mechanistic studies of the cobalt(II) complex 1 involved apoptosis assay, cell cycle analysis, dichloro-dihydro-fluorescein diacetate assay, intracellular reactive oxygen species assay and proteasome inhibition assay. Complex 1 induced low apoptosis, generated low level of ROS and did not inhibit proteasome in normal cells. The study of the DNA binding and nucleolytic properties of complexes 1-3 in the absence or presence of H2O2 or sodium ascorbate revealed that only complex 1 was not nucleolytic. 2021 Article PeerReviewed Ng, Chew Hee and Tan, Thean Heng and Tioh, Ngee Heng and Seng, Hoi Ling and Ahmad, Munirah and Ng, Seik Weng and Gan, Wei Khang and Low, May Lee and Lai, Jing Wei and Zulkefeli, Mohd (2021) Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes. Journal of Inorganic Biochemistry, 220. p. 111453. ISSN 01620134, DOI https://doi.org/10.1016/j.jinorgbio.2021.111453 <https://doi.org/10.1016/j.jinorgbio.2021.111453>. https://doi.org/10.1016/j.jinorgbio.2021.111453 doi:10.1016/j.jinorgbio.2021.111453
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Ng, Chew Hee
Tan, Thean Heng
Tioh, Ngee Heng
Seng, Hoi Ling
Ahmad, Munirah
Ng, Seik Weng
Gan, Wei Khang
Low, May Lee
Lai, Jing Wei
Zulkefeli, Mohd
Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
description The cobalt(II), copper(II) and zinc(II) complexes of 1,10-phenanthroline (phen) and maltol (mal) (complexes 1, 2, 3 respectively) were prepared from their respective metal(II) chlorides and were characterized by FT-IR, elemental analysis, UV spectroscopy, molar conductivity, p-nitrosodimethylaniline assay and mass spectrometry. The X-ray structure of a single crystal of the zinc(II) analogue reveals a square pyramidal structure with distinctly shorter apical chloride bond. All complexes were evaluated for their anticancer property on breast cancer cell lines MCF-7 and MDA-MB-231, and normal cell line MCF-10A, using (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide assay and morphological studies. Complex 2 was most potent for 24, 48 and 72 h treatment of cancer cells but it was not selective towards cancer over normal cells. The mechanistic studies of the cobalt(II) complex 1 involved apoptosis assay, cell cycle analysis, dichloro-dihydro-fluorescein diacetate assay, intracellular reactive oxygen species assay and proteasome inhibition assay. Complex 1 induced low apoptosis, generated low level of ROS and did not inhibit proteasome in normal cells. The study of the DNA binding and nucleolytic properties of complexes 1-3 in the absence or presence of H2O2 or sodium ascorbate revealed that only complex 1 was not nucleolytic.
format Article
author Ng, Chew Hee
Tan, Thean Heng
Tioh, Ngee Heng
Seng, Hoi Ling
Ahmad, Munirah
Ng, Seik Weng
Gan, Wei Khang
Low, May Lee
Lai, Jing Wei
Zulkefeli, Mohd
author_facet Ng, Chew Hee
Tan, Thean Heng
Tioh, Ngee Heng
Seng, Hoi Ling
Ahmad, Munirah
Ng, Seik Weng
Gan, Wei Khang
Low, May Lee
Lai, Jing Wei
Zulkefeli, Mohd
author_sort Ng, Chew Hee
title Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
title_short Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
title_full Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
title_fullStr Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
title_full_unstemmed Synthesis, characterization and multiple targeting with selectivity: Anticancer property of ternary metal phenanthroline-maltol complexes
title_sort synthesis, characterization and multiple targeting with selectivity: anticancer property of ternary metal phenanthroline-maltol complexes
publishDate 2021
url http://eprints.um.edu.my/26211/
https://doi.org/10.1016/j.jinorgbio.2021.111453
_version_ 1735409385926033408
score 13.211869

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