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Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii

A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB str...

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Main Authors: Yap, Phui Chyng, Ayuhan, Noorfazlin, Woon, Jia Jie, Teh, Cindy Shuan Ju, Lee, Vannajan Sanghiran, Azman, Adzzie Shazleen, AbuBakar, Sazaly, Lee, Hai Yen
Format: Article
Published: MDPI 2021
Subjects:
QD Chemistry
R Medicine
Online Access:http://eprints.um.edu.my/25908/
https://doi.org/10.3390/molecules26061727
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spelling my.um.eprints.259082021-04-30T01:29:37Z http://eprints.um.edu.my/25908/ Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii Yap, Phui Chyng Ayuhan, Noorfazlin Woon, Jia Jie Teh, Cindy Shuan Ju Lee, Vannajan Sanghiran Azman, Adzzie Shazleen AbuBakar, Sazaly Lee, Hai Yen QD Chemistry R Medicine A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico. MDPI 2021 Article PeerReviewed Yap, Phui Chyng and Ayuhan, Noorfazlin and Woon, Jia Jie and Teh, Cindy Shuan Ju and Lee, Vannajan Sanghiran and Azman, Adzzie Shazleen and AbuBakar, Sazaly and Lee, Hai Yen (2021) Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii. Molecules, 26 (6). p. 1727. ISSN 1420-3049 https://doi.org/10.3390/molecules26061727 doi:10.3390/molecules26061727
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
R Medicine
spellingShingle QD Chemistry
R Medicine
Yap, Phui Chyng
Ayuhan, Noorfazlin
Woon, Jia Jie
Teh, Cindy Shuan Ju
Lee, Vannajan Sanghiran
Azman, Adzzie Shazleen
AbuBakar, Sazaly
Lee, Hai Yen
Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
description A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
format Article
author Yap, Phui Chyng
Ayuhan, Noorfazlin
Woon, Jia Jie
Teh, Cindy Shuan Ju
Lee, Vannajan Sanghiran
Azman, Adzzie Shazleen
AbuBakar, Sazaly
Lee, Hai Yen
author_facet Yap, Phui Chyng
Ayuhan, Noorfazlin
Woon, Jia Jie
Teh, Cindy Shuan Ju
Lee, Vannajan Sanghiran
Azman, Adzzie Shazleen
AbuBakar, Sazaly
Lee, Hai Yen
author_sort Yap, Phui Chyng
title Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
title_short Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
title_full Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
title_fullStr Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
title_full_unstemmed Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
title_sort profiling of potential antibacterial compounds of lactic acid bacteria against extremely drug resistant (xdr) acinetobacter baumannii
publisher MDPI
publishDate 2021
url http://eprints.um.edu.my/25908/
https://doi.org/10.3390/molecules26061727
_version_ 1698697323259887616
score 13.211869

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