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Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases

Anti-latent membrane protein-1 (LMP-1) is an EBV-encoded type III integral membrane protein with oncogenic potential that is expressed most consistently in various EBV-associated malignancies. Unlike many other EBV proteins, LMP-1 Abs have rarely been demonstrated in EBV-associated disease condition...

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Main Authors: Xu, Jingwu, Ahmad, Ali, D’Addario, Mario, Knafo, Laurent, Jones, James F., Prasad, Umapati, Dolcetti, R., Vaccher, E., Menezes, José
Format: Article
Published: American Association of Immunologists 2000
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R Medicine
Online Access:http://eprints.um.edu.my/25855/
https://doi.org/10.4049/jimmunol.164.5.2815
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spelling my.um.eprints.258552021-04-12T01:56:14Z http://eprints.um.edu.my/25855/ Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases Xu, Jingwu Ahmad, Ali D’Addario, Mario Knafo, Laurent Jones, James F. Prasad, Umapati Dolcetti, R. Vaccher, E. Menezes, José R Medicine Anti-latent membrane protein-1 (LMP-1) is an EBV-encoded type III integral membrane protein with oncogenic potential that is expressed most consistently in various EBV-associated malignancies. Unlike many other EBV proteins, LMP-1 Abs have rarely been demonstrated in EBV-associated disease conditions. We established a high level LMP-1-expressing cell clone and used it for the detection, quantitation, and characterization of these Abs in various human sera in immunoblots and ELISA. Our results demonstrate that, in contrast to the commonly held notion, LMP-1 induces significant humoral immune responses in EBV-associated malignant conditions especially in nasopharyngeal carcinoma (NPC) patients in whom >70% sera are positive for these Abs, and their titers correlate with the clinical condition of the tumors. Interestingly, anti-LMP-1 Abs of IgA isotype were found only in NPC patients. These Abs were absent from the sera of infectious mononucleosis and chronic EBV infection patients, whereas a small fraction (~5%) of the healthy, EBV-seropositive individuals were positive for them; however, their OD values were much lower than those of NPC patients. These studies demonstrate, for the first time, the potential significance of LMP-1-specific Abs for the diagnosis and prognosis of EBV-associated malignancies, especially of NPC. American Association of Immunologists 2000 Article PeerReviewed Xu, Jingwu and Ahmad, Ali and D’Addario, Mario and Knafo, Laurent and Jones, James F. and Prasad, Umapati and Dolcetti, R. and Vaccher, E. and Menezes, José (2000) Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases. The Journal of Immunology, 164 (5). pp. 2815-2822. ISSN 0022-1767 https://doi.org/10.4049/jimmunol.164.5.2815 doi:10.4049/jimmunol.164.5.2815
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Xu, Jingwu
Ahmad, Ali
D’Addario, Mario
Knafo, Laurent
Jones, James F.
Prasad, Umapati
Dolcetti, R.
Vaccher, E.
Menezes, José
Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
description Anti-latent membrane protein-1 (LMP-1) is an EBV-encoded type III integral membrane protein with oncogenic potential that is expressed most consistently in various EBV-associated malignancies. Unlike many other EBV proteins, LMP-1 Abs have rarely been demonstrated in EBV-associated disease conditions. We established a high level LMP-1-expressing cell clone and used it for the detection, quantitation, and characterization of these Abs in various human sera in immunoblots and ELISA. Our results demonstrate that, in contrast to the commonly held notion, LMP-1 induces significant humoral immune responses in EBV-associated malignant conditions especially in nasopharyngeal carcinoma (NPC) patients in whom >70% sera are positive for these Abs, and their titers correlate with the clinical condition of the tumors. Interestingly, anti-LMP-1 Abs of IgA isotype were found only in NPC patients. These Abs were absent from the sera of infectious mononucleosis and chronic EBV infection patients, whereas a small fraction (~5%) of the healthy, EBV-seropositive individuals were positive for them; however, their OD values were much lower than those of NPC patients. These studies demonstrate, for the first time, the potential significance of LMP-1-specific Abs for the diagnosis and prognosis of EBV-associated malignancies, especially of NPC.
format Article
author Xu, Jingwu
Ahmad, Ali
D’Addario, Mario
Knafo, Laurent
Jones, James F.
Prasad, Umapati
Dolcetti, R.
Vaccher, E.
Menezes, José
author_facet Xu, Jingwu
Ahmad, Ali
D’Addario, Mario
Knafo, Laurent
Jones, James F.
Prasad, Umapati
Dolcetti, R.
Vaccher, E.
Menezes, José
author_sort Xu, Jingwu
title Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
title_short Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
title_full Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
title_fullStr Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
title_full_unstemmed Analysis and Significance of Anti-Latent Membrane Protein-1 Antibodies in the Sera of Patients with EBV-Associated Diseases
title_sort analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with ebv-associated diseases
publisher American Association of Immunologists
publishDate 2000
url http://eprints.um.edu.my/25855/
https://doi.org/10.4049/jimmunol.164.5.2815
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score 13.211869

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