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Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model

The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) de...

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Main Authors: Yu, Shu Pin, Ong, Kien Chai, Tan, Soon Hao, Perera, David, Wong, Kum Thong
Format: Article
Published: ASEAN Neurological Association 2020
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/25742/
https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf
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spelling my.um.eprints.257422021-02-10T00:49:13Z http://eprints.um.edu.my/25742/ Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model Yu, Shu Pin Ong, Kien Chai Tan, Soon Hao Perera, David Wong, Kum Thong R Medicine The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. © 2020, ASEAN Neurological Association. All rights reserved. ASEAN Neurological Association 2020 Article PeerReviewed Yu, Shu Pin and Ong, Kien Chai and Tan, Soon Hao and Perera, David and Wong, Kum Thong (2020) Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model. Neurology Asia, 25 (3). pp. 279-284. ISSN 1823-6138 https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kum Thong
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
description The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. © 2020, ASEAN Neurological Association. All rights reserved.
format Article
author Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kum Thong
author_facet Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kum Thong
author_sort Yu, Shu Pin
title Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_short Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_full Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_fullStr Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_full_unstemmed Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_sort japanese encephalitis virus: biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
publisher ASEAN Neurological Association
publishDate 2020
url http://eprints.um.edu.my/25742/
https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf
_version_ 1691733434163003392
score 13.211869

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