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Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection

Tioman virus (TioPV) and Menangle virus (MenPV) are two antigenically and genetically related paramyxoviruses (genus: Rubulavirus, family: Paramyxoviridae) isolated from Peninsular Malaysia (2001) and Australia (1997), respectively. Both viruses are potential zoonotic agents. In the present study, t...

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Main Authors: Yaiw, Koon Chu, Hyatt, Alex, VanDriel, R., Crameri, Sandra G., Eaton, Bryan, Wong, M.H., Wang, L.F., Ng, Mah Lee, Bingham, John, Shamala, Devi, Wong, Kum Thong
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Published: Springer Verlag (Germany) 2008
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R Medicine
Online Access:http://eprints.um.edu.my/25486/
https://doi.org/10.1007/s00705-008-0059-0
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spelling my.um.eprints.254862020-09-01T04:08:42Z http://eprints.um.edu.my/25486/ Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection Yaiw, Koon Chu Hyatt, Alex VanDriel, R. Crameri, Sandra G. Eaton, Bryan Wong, M.H. Wang, L.F. Ng, Mah Lee Bingham, John Shamala, Devi Wong, Kum Thong R Medicine Tioman virus (TioPV) and Menangle virus (MenPV) are two antigenically and genetically related paramyxoviruses (genus: Rubulavirus, family: Paramyxoviridae) isolated from Peninsular Malaysia (2001) and Australia (1997), respectively. Both viruses are potential zoonotic agents. In the present study, the infectivity, growth kinetics, morphology and morphogenesis of these two paramyxoviruses in a human neuronal cell (SK-N-SH) line were investigated. Sub-confluent SK-N-SH cells were infected with TioPV and MenPV at similar multiplicity of infection. These cells were examined by conventional and immunoelectron microscopy, and virus titres in the supernatants were assayed. Syncytia were observed for both infections in SK-N-SH cells and were more pronounced during the early stages of TioPV infection. The TioPV titre increased consistently (101) every 12 h after infection. In MenPV-infected cells, cellular material was frequently observed within budding virions, and microfilaments and microtubules were abundant. Viral budding was common, and extracellular MenPVs tended to be more pleomorphic compared to TioPVs, which appeared to be more spherical in appearance. The MenPV cytoplasmic viral inclusion appeared to be comparatively smaller, loose and interspersed with randomly scattered circle-like particles, whereas huge tubule-like cytoplasmic inclusions were observed in TioPV-infected cells. Both viruses also displayed different cellular pathology in the SK-N-SH cells. The intracellular ultrastructural characteristics of these two viruses in infected neuronal cells may allow them to be differentiated by electron microscopy. © 2008 Springer-Verlag. Springer Verlag (Germany) 2008 Article PeerReviewed Yaiw, Koon Chu and Hyatt, Alex and VanDriel, R. and Crameri, Sandra G. and Eaton, Bryan and Wong, M.H. and Wang, L.F. and Ng, Mah Lee and Bingham, John and Shamala, Devi and Wong, Kum Thong (2008) Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection. Archives of Virology, 153 (5). pp. 865-875. ISSN 0304-8608 https://doi.org/10.1007/s00705-008-0059-0 doi:10.1007/s00705-008-0059-0
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Yaiw, Koon Chu
Hyatt, Alex
VanDriel, R.
Crameri, Sandra G.
Eaton, Bryan
Wong, M.H.
Wang, L.F.
Ng, Mah Lee
Bingham, John
Shamala, Devi
Wong, Kum Thong
Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
description Tioman virus (TioPV) and Menangle virus (MenPV) are two antigenically and genetically related paramyxoviruses (genus: Rubulavirus, family: Paramyxoviridae) isolated from Peninsular Malaysia (2001) and Australia (1997), respectively. Both viruses are potential zoonotic agents. In the present study, the infectivity, growth kinetics, morphology and morphogenesis of these two paramyxoviruses in a human neuronal cell (SK-N-SH) line were investigated. Sub-confluent SK-N-SH cells were infected with TioPV and MenPV at similar multiplicity of infection. These cells were examined by conventional and immunoelectron microscopy, and virus titres in the supernatants were assayed. Syncytia were observed for both infections in SK-N-SH cells and were more pronounced during the early stages of TioPV infection. The TioPV titre increased consistently (101) every 12 h after infection. In MenPV-infected cells, cellular material was frequently observed within budding virions, and microfilaments and microtubules were abundant. Viral budding was common, and extracellular MenPVs tended to be more pleomorphic compared to TioPVs, which appeared to be more spherical in appearance. The MenPV cytoplasmic viral inclusion appeared to be comparatively smaller, loose and interspersed with randomly scattered circle-like particles, whereas huge tubule-like cytoplasmic inclusions were observed in TioPV-infected cells. Both viruses also displayed different cellular pathology in the SK-N-SH cells. The intracellular ultrastructural characteristics of these two viruses in infected neuronal cells may allow them to be differentiated by electron microscopy. © 2008 Springer-Verlag.
format Article
author Yaiw, Koon Chu
Hyatt, Alex
VanDriel, R.
Crameri, Sandra G.
Eaton, Bryan
Wong, M.H.
Wang, L.F.
Ng, Mah Lee
Bingham, John
Shamala, Devi
Wong, Kum Thong
author_facet Yaiw, Koon Chu
Hyatt, Alex
VanDriel, R.
Crameri, Sandra G.
Eaton, Bryan
Wong, M.H.
Wang, L.F.
Ng, Mah Lee
Bingham, John
Shamala, Devi
Wong, Kum Thong
author_sort Yaiw, Koon Chu
title Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
title_short Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
title_full Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
title_fullStr Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
title_full_unstemmed Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection
title_sort viral morphogenesis and morphological changes in human neuronal cells following tioman and menangle virus infection
publisher Springer Verlag (Germany)
publishDate 2008
url http://eprints.um.edu.my/25486/
https://doi.org/10.1007/s00705-008-0059-0
_version_ 1680857037330710528
score 13.211869

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