Cover Image

Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens

Antibacterial titanium dioxide nanoparticles (TiO2NPs) were biologically synthesized by microwave irradiation of Cissus quadrangularis extract followed by calcination at 450 °C for 3 h. As-synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction anal...

Full description

Saved in:
Bibliographic Details
Main Authors: Priyadarshini, S., Mainal, Azizah, Sonsudin, Faridah, Yahya, Rosiyah, Alyousef, Abdullah A., Mohammed, Arshad
Format: Article
Published: Springer Verlag 2020
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://eprints.um.edu.my/25382/
https://doi.org/10.1007/s11164-019-03857-6
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.25382
record_format eprints
spelling my.um.eprints.253822020-08-17T04:18:03Z http://eprints.um.edu.my/25382/ Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens Priyadarshini, S. Mainal, Azizah Sonsudin, Faridah Yahya, Rosiyah Alyousef, Abdullah A. Mohammed, Arshad Q Science (General) QD Chemistry Antibacterial titanium dioxide nanoparticles (TiO2NPs) were biologically synthesized by microwave irradiation of Cissus quadrangularis extract followed by calcination at 450 °C for 3 h. As-synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction analysis, and high-resolution transmission electron microscopy. The bioactivity of TiO2NPs against human nosocomial Gram-positive and Gram-negative bacterial pathogens (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) in liquid media was analyzed in the dark and under ultraviolet (UV, 365 nm) illumination. TiO2NPs demonstrated inhibitory effects against bacteria in dark, and enhanced bactericidal effect under UV illumination. Photoactivation generated holes (h+) and electrons (e−) on the surface of the TiO2NPs, inducing hydroxyl (OH−) and superoxide free radicals (.O2 −) reactive oxygen species (ROS) on the bacterial surface. Furthermore, the interaction between the pathogenic bacteria and TiO2NPs was investigated by bacterial killing assay, colony counting, and viability assay using confocal microscopy. The experimental results revealed that the photoenhanced antibacterial effect of TiO2NPs makes them worth considering as a disinfectant for hospital garments. © 2019, Springer Nature B.V. Springer Verlag 2020 Article PeerReviewed Priyadarshini, S. and Mainal, Azizah and Sonsudin, Faridah and Yahya, Rosiyah and Alyousef, Abdullah A. and Mohammed, Arshad (2020) Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens. Research on Chemical Intermediates, 46 (2). pp. 1077-1089. ISSN 0922-6168 https://doi.org/10.1007/s11164-019-03857-6 doi:10.1007/s11164-019-03857-6
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Priyadarshini, S.
Mainal, Azizah
Sonsudin, Faridah
Yahya, Rosiyah
Alyousef, Abdullah A.
Mohammed, Arshad
Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
description Antibacterial titanium dioxide nanoparticles (TiO2NPs) were biologically synthesized by microwave irradiation of Cissus quadrangularis extract followed by calcination at 450 °C for 3 h. As-synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction analysis, and high-resolution transmission electron microscopy. The bioactivity of TiO2NPs against human nosocomial Gram-positive and Gram-negative bacterial pathogens (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) in liquid media was analyzed in the dark and under ultraviolet (UV, 365 nm) illumination. TiO2NPs demonstrated inhibitory effects against bacteria in dark, and enhanced bactericidal effect under UV illumination. Photoactivation generated holes (h+) and electrons (e−) on the surface of the TiO2NPs, inducing hydroxyl (OH−) and superoxide free radicals (.O2 −) reactive oxygen species (ROS) on the bacterial surface. Furthermore, the interaction between the pathogenic bacteria and TiO2NPs was investigated by bacterial killing assay, colony counting, and viability assay using confocal microscopy. The experimental results revealed that the photoenhanced antibacterial effect of TiO2NPs makes them worth considering as a disinfectant for hospital garments. © 2019, Springer Nature B.V.
format Article
author Priyadarshini, S.
Mainal, Azizah
Sonsudin, Faridah
Yahya, Rosiyah
Alyousef, Abdullah A.
Mohammed, Arshad
author_facet Priyadarshini, S.
Mainal, Azizah
Sonsudin, Faridah
Yahya, Rosiyah
Alyousef, Abdullah A.
Mohammed, Arshad
author_sort Priyadarshini, S.
title Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
title_short Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
title_full Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
title_fullStr Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
title_full_unstemmed Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
title_sort biosynthesis of tio2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens
publisher Springer Verlag
publishDate 2020
url http://eprints.um.edu.my/25382/
https://doi.org/10.1007/s11164-019-03857-6
_version_ 1680857025899134976
score 13.211869

Similar Items