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Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction

Microfluidic system is a useful alternative for the determination of enzyme kinetic parameter values from minute amounts of pure enzyme and substrate(s) requirement. Lipase reaction was comparatively studied in microfluidic system alongside 1-cm cuvette and cylindrical vessel. Kinetic parameter (Km,...

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Main Authors: Razak, N.N.A., Firmansyah, S., Annuar, Mohamad Suffian Mohamad
Format: Article
Published: Elsevier 2020
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/25316/
https://doi.org/10.1016/j.bcab.2020.101660
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spelling my.um.eprints.253162020-08-12T01:07:05Z http://eprints.um.edu.my/25316/ Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction Razak, N.N.A. Firmansyah, S. Annuar, Mohamad Suffian Mohamad Q Science (General) QH Natural history Microfluidic system is a useful alternative for the determination of enzyme kinetic parameter values from minute amounts of pure enzyme and substrate(s) requirement. Lipase reaction was comparatively studied in microfluidic system alongside 1-cm cuvette and cylindrical vessel. Kinetic parameter (Km, kcat, Vmax, kcat/Km) values determined in them were compared. Different geometries of micromixers yielded comparable Km values, representing intermediate values between cuvette and cylindrincal vessel. Comparable values for kcat (≅ Vmax) were obtained for cuvette and cylindrical vessel. kcat (≅ Vmax) values were found to decrease in the micromixers compared to the batch system. Highest specificity kcat/Km was determined in the cuvette system whilst comparable for the rest of studied systems. The observed differences in kinetic parameter values were discussed against prevailing hydrodynamics in the different systems. The findings highlighted important implication in employing different systems to determine enzyme kinetic parameter values. © 2020 Elsevier Ltd Elsevier 2020 Article PeerReviewed Razak, N.N.A. and Firmansyah, S. and Annuar, Mohamad Suffian Mohamad (2020) Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction. Biocatalysis and Agricultural Biotechnology, 27. p. 101660. ISSN 1878-8181 https://doi.org/10.1016/j.bcab.2020.101660 doi:10.1016/j.bcab.2020.101660
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Razak, N.N.A.
Firmansyah, S.
Annuar, Mohamad Suffian Mohamad
Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
description Microfluidic system is a useful alternative for the determination of enzyme kinetic parameter values from minute amounts of pure enzyme and substrate(s) requirement. Lipase reaction was comparatively studied in microfluidic system alongside 1-cm cuvette and cylindrical vessel. Kinetic parameter (Km, kcat, Vmax, kcat/Km) values determined in them were compared. Different geometries of micromixers yielded comparable Km values, representing intermediate values between cuvette and cylindrincal vessel. Comparable values for kcat (≅ Vmax) were obtained for cuvette and cylindrical vessel. kcat (≅ Vmax) values were found to decrease in the micromixers compared to the batch system. Highest specificity kcat/Km was determined in the cuvette system whilst comparable for the rest of studied systems. The observed differences in kinetic parameter values were discussed against prevailing hydrodynamics in the different systems. The findings highlighted important implication in employing different systems to determine enzyme kinetic parameter values. © 2020 Elsevier Ltd
format Article
author Razak, N.N.A.
Firmansyah, S.
Annuar, Mohamad Suffian Mohamad
author_facet Razak, N.N.A.
Firmansyah, S.
Annuar, Mohamad Suffian Mohamad
author_sort Razak, N.N.A.
title Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
title_short Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
title_full Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
title_fullStr Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
title_full_unstemmed Effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
title_sort effects of microfluidization on kinetic parameter values of lipase hydrolysis reaction
publisher Elsevier
publishDate 2020
url http://eprints.um.edu.my/25316/
https://doi.org/10.1016/j.bcab.2020.101660
_version_ 1680857018120798208
score 13.211869

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