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Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications

Background aims. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good man...

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Main Authors:	Govindasamy, Vijayendran, Ronald, Veronica Sainik, Abdullah, Aimi Naim, Nathan, Kavitha R. Ganesan, Che Ab Aziz, Zeti Adura, Abdullah, Mariam, Zain, R.B., Abu Kasim, Noor Hayaty, Musa, Sabri, Bhonde, Ramesh R.
Format:	Article
Language:	English
Published:	Elsevier 2011
Subjects:	R Medicine R Medicine (General) RK Dentistry
Online Access:	http://eprints.um.edu.my/2471/1/Human_platelet_lysate_permits_scale.pdf http://eprints.um.edu.my/2471/ https://doi.org/10.3109/14653249.2011.602337
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spelling	my.um.eprints.24712019-11-12T03:57:15Z http://eprints.um.edu.my/2471/ Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications Govindasamy, Vijayendran Ronald, Veronica Sainik Abdullah, Aimi Naim Nathan, Kavitha R. Ganesan Che Ab Aziz, Zeti Adura Abdullah, Mariam Zain, R.B. Abu Kasim, Noor Hayaty Musa, Sabri Bhonde, Ramesh R. R Medicine R Medicine (General) RK Dentistry Background aims. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS is an undesirable additive to cells because it carries the risk of transmitting viral and prion diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in a large-scale set-up. Methods. We expanded the DPSC in Dulbecco's modified Eagle's medium–knock-out (DMEM-KO) with either 10% FBS or 10% HPL, and studied the characteristics of DPSC at pre- (T25 culture flask) and post- (5-STACK chamber) large-scale expansion in terms of their identity, quality, functionality, molecular signatures and cytogenetic stability. Results. In both pre- and post-large-scale expansion, DPSC expanded in HPL showed extensive proliferation of cells (c. 2-fold) compared with FBS; the purity, immune phenotype, colony-forming unit potential and differentiation were comparable. Furthermore, to understand the gene expression profiling, the transcriptomes and cytogenetics of DPSC expanded under HPL and FBS were compared, revealing similar expression profiles. Conclusions. We present a highly economized expansion of DPSC in HPL, yielding double the amount of cells while retaining their basic characteristics during a shorter time period under cGMP conditions, making it suitable for therapeutic applications. Elsevier 2011-11 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2471/1/Human_platelet_lysate_permits_scale.pdf Govindasamy, Vijayendran and Ronald, Veronica Sainik and Abdullah, Aimi Naim and Nathan, Kavitha R. Ganesan and Che Ab Aziz, Zeti Adura and Abdullah, Mariam and Zain, R.B. and Abu Kasim, Noor Hayaty and Musa, Sabri and Bhonde, Ramesh R. (2011) Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications. Cytotherapy, 13 (10). pp. 1221-1233. ISSN 1465-3249 https://doi.org/10.3109/14653249.2011.602337 doi:10.3109/14653249.2011.602337
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
language	English
topic	R Medicine R Medicine (General) RK Dentistry
spellingShingle	R Medicine R Medicine (General) RK Dentistry Govindasamy, Vijayendran Ronald, Veronica Sainik Abdullah, Aimi Naim Nathan, Kavitha R. Ganesan Che Ab Aziz, Zeti Adura Abdullah, Mariam Zain, R.B. Abu Kasim, Noor Hayaty Musa, Sabri Bhonde, Ramesh R. Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
description	Background aims. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS is an undesirable additive to cells because it carries the risk of transmitting viral and prion diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in a large-scale set-up. Methods. We expanded the DPSC in Dulbecco's modified Eagle's medium–knock-out (DMEM-KO) with either 10% FBS or 10% HPL, and studied the characteristics of DPSC at pre- (T25 culture flask) and post- (5-STACK chamber) large-scale expansion in terms of their identity, quality, functionality, molecular signatures and cytogenetic stability. Results. In both pre- and post-large-scale expansion, DPSC expanded in HPL showed extensive proliferation of cells (c. 2-fold) compared with FBS; the purity, immune phenotype, colony-forming unit potential and differentiation were comparable. Furthermore, to understand the gene expression profiling, the transcriptomes and cytogenetics of DPSC expanded under HPL and FBS were compared, revealing similar expression profiles. Conclusions. We present a highly economized expansion of DPSC in HPL, yielding double the amount of cells while retaining their basic characteristics during a shorter time period under cGMP conditions, making it suitable for therapeutic applications.
format	Article
author	Govindasamy, Vijayendran Ronald, Veronica Sainik Abdullah, Aimi Naim Nathan, Kavitha R. Ganesan Che Ab Aziz, Zeti Adura Abdullah, Mariam Zain, R.B. Abu Kasim, Noor Hayaty Musa, Sabri Bhonde, Ramesh R.
author_facet	Govindasamy, Vijayendran Ronald, Veronica Sainik Abdullah, Aimi Naim Nathan, Kavitha R. Ganesan Che Ab Aziz, Zeti Adura Abdullah, Mariam Zain, R.B. Abu Kasim, Noor Hayaty Musa, Sabri Bhonde, Ramesh R.
author_sort	Govindasamy, Vijayendran
title	Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
title_short	Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
title_full	Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
title_fullStr	Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
title_full_unstemmed	Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
title_sort	human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications
publisher	Elsevier
publishDate	2011
url	http://eprints.um.edu.my/2471/1/Human_platelet_lysate_permits_scale.pdf http://eprints.um.edu.my/2471/ https://doi.org/10.3109/14653249.2011.602337
_version_	1651867307320803328
score	13.211869

Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications

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