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A simple adherence test for detection of IgM antibodies in typhoid

A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typh...

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Main Authors: Ong, L.Y., Pang, T., Lim, S.H., Tan, E.L., Puthucheary, S.D.
Format: Article
Published: Microbiology Society 1989
Subjects:
R Medicine (General)
Online Access:http://eprints.um.edu.my/246/
http://www.ncbi.nlm.nih.gov/pubmed/2473209
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spelling my.um.eprints.2462019-04-18T07:57:40Z http://eprints.um.edu.my/246/ A simple adherence test for detection of IgM antibodies in typhoid Ong, L.Y. Pang, T. Lim, S.H. Tan, E.L. Puthucheary, S.D. R Medicine (General) A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed. Microbiology Society 1989-07 Article PeerReviewed Ong, L.Y. and Pang, T. and Lim, S.H. and Tan, E.L. and Puthucheary, S.D. (1989) A simple adherence test for detection of IgM antibodies in typhoid. Journal of Medical Microbiology, 29 (3). pp. 195-8. ISSN 0022-2615 http://www.ncbi.nlm.nih.gov/pubmed/2473209 2473209
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Ong, L.Y.
Pang, T.
Lim, S.H.
Tan, E.L.
Puthucheary, S.D.
A simple adherence test for detection of IgM antibodies in typhoid
description A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed.
format Article
author Ong, L.Y.
Pang, T.
Lim, S.H.
Tan, E.L.
Puthucheary, S.D.
author_facet Ong, L.Y.
Pang, T.
Lim, S.H.
Tan, E.L.
Puthucheary, S.D.
author_sort Ong, L.Y.
title A simple adherence test for detection of IgM antibodies in typhoid
title_short A simple adherence test for detection of IgM antibodies in typhoid
title_full A simple adherence test for detection of IgM antibodies in typhoid
title_fullStr A simple adherence test for detection of IgM antibodies in typhoid
title_full_unstemmed A simple adherence test for detection of IgM antibodies in typhoid
title_sort simple adherence test for detection of igm antibodies in typhoid
publisher Microbiology Society
publishDate 1989
url http://eprints.um.edu.my/246/
http://www.ncbi.nlm.nih.gov/pubmed/2473209
_version_ 1643686543720710144
score 13.211869

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