Cover Image

Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction

Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously a...

Full description

Saved in:
Bibliographic Details
Main Authors: Wee, Yong Chui, Tan, Kim Lian, Tan, Peng Chiong, Yap, S.F.
Format: Article
Language:English
Published: Malaysian Medical Association 2005
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf
http://eprints.um.edu.my/24542/
http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.24542
record_format eprints
spelling my.um.eprints.245422020-07-20T03:50:17Z http://eprints.um.edu.my/24542/ Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction Wee, Yong Chui Tan, Kim Lian Tan, Peng Chiong Yap, S.F. R Medicine Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously amplifies a normal 136 bp sequence between the psialpha-alpha2-globin genes and a 730 bp Southeast Asian deletion-specific sequence (--SEA) between the psialpha2-theta1-globin genes was established. The duplex-PCR which detects the --SEA deletion in both chromosomes serves as a rapid and cost-effective confirmatory test in the antenatal diagnosis of Haemoglobin Bart's hydrops foetalis syndrome in Malaysia. In addition, the duplex-PCR is simple to perform as both the normal and deletion-specific alpha-globin gene sequences are amplified in the same PCR reaction. Malaysian Medical Association 2005 Article PeerReviewed text en http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf Wee, Yong Chui and Tan, Kim Lian and Tan, Peng Chiong and Yap, S.F. (2005) Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction. Medical Journal of Malaysia, 60 (4). pp. 447-453. ISSN 0300-5283 http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Wee, Yong Chui
Tan, Kim Lian
Tan, Peng Chiong
Yap, S.F.
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
description Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously amplifies a normal 136 bp sequence between the psialpha-alpha2-globin genes and a 730 bp Southeast Asian deletion-specific sequence (--SEA) between the psialpha2-theta1-globin genes was established. The duplex-PCR which detects the --SEA deletion in both chromosomes serves as a rapid and cost-effective confirmatory test in the antenatal diagnosis of Haemoglobin Bart's hydrops foetalis syndrome in Malaysia. In addition, the duplex-PCR is simple to perform as both the normal and deletion-specific alpha-globin gene sequences are amplified in the same PCR reaction.
format Article
author Wee, Yong Chui
Tan, Kim Lian
Tan, Peng Chiong
Yap, S.F.
author_facet Wee, Yong Chui
Tan, Kim Lian
Tan, Peng Chiong
Yap, S.F.
author_sort Wee, Yong Chui
title Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
title_short Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
title_full Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
title_fullStr Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
title_full_unstemmed Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
title_sort rapid and cost-effective antenatal diagnosis of haemoglobin bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
publisher Malaysian Medical Association
publishDate 2005
url http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf
http://eprints.um.edu.my/24542/
http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf
_version_ 1674066722658713600
score 13.211869

Similar Items