Cover Image

An immunohistochemical method for the diagnosis of melioidosis

In order to assess the usefulness of immunohistochemistry in the diagnosis of melioidosis, an infection by Burkholderia pseudomallei, polyclonal antibodies were applied to tissues from known cases of melioidosis and to other infected tissues. Formalin-fixed, paraffin-embedded tissues were stained by...

Full description

Saved in:
Bibliographic Details
Main Authors: Wong, K.T., Vadivelu, J., Puthucheary, S.D., Tan, K.L.
Format: Article
Published: Faculty of Medicine, University of Malaya 1996
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/24486/
https://jummec.um.edu.my/issue/view/661/Vol.1%2C%20No.2%2C%201996
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.24486
record_format eprints
spelling my.um.eprints.244862021-03-24T01:40:59Z http://eprints.um.edu.my/24486/ An immunohistochemical method for the diagnosis of melioidosis Wong, K.T. Vadivelu, J. Puthucheary, S.D. Tan, K.L. R Medicine In order to assess the usefulness of immunohistochemistry in the diagnosis of melioidosis, an infection by Burkholderia pseudomallei, polyclonal antibodies were applied to tissues from known cases of melioidosis and to other infected tissues. Formalin-fixed, paraffin-embedded tissues were stained by a modified immunoperoxidase technique. In autopsy tissues with inflammatory lesions of melioidosis, the cytoplasm of phagocytes and intact bacilli, both intra- and extracellular, were stained very strongly positive. Relatively more focal positive staining was observed in some but not all surgical biopsies from proven cases of melioidosis. In granulomas staining was mainly found in the central necrotic areas, with little staining of peripheral phagocytes. All control materials stained negative. Immunohistochemistry appears to be a useful diagnostic tool in melioidosis. Faculty of Medicine, University of Malaya 1996-12 Article PeerReviewed Wong, K.T. and Vadivelu, J. and Puthucheary, S.D. and Tan, K.L. (1996) An immunohistochemical method for the diagnosis of melioidosis. Journal of the University of Malaya Medical Centre (JUMMEC), 1 (2). p. 59. ISSN 1823-7339 https://jummec.um.edu.my/issue/view/661/Vol.1%2C%20No.2%2C%201996
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Wong, K.T.
Vadivelu, J.
Puthucheary, S.D.
Tan, K.L.
An immunohistochemical method for the diagnosis of melioidosis
description In order to assess the usefulness of immunohistochemistry in the diagnosis of melioidosis, an infection by Burkholderia pseudomallei, polyclonal antibodies were applied to tissues from known cases of melioidosis and to other infected tissues. Formalin-fixed, paraffin-embedded tissues were stained by a modified immunoperoxidase technique. In autopsy tissues with inflammatory lesions of melioidosis, the cytoplasm of phagocytes and intact bacilli, both intra- and extracellular, were stained very strongly positive. Relatively more focal positive staining was observed in some but not all surgical biopsies from proven cases of melioidosis. In granulomas staining was mainly found in the central necrotic areas, with little staining of peripheral phagocytes. All control materials stained negative. Immunohistochemistry appears to be a useful diagnostic tool in melioidosis.
format Article
author Wong, K.T.
Vadivelu, J.
Puthucheary, S.D.
Tan, K.L.
author_facet Wong, K.T.
Vadivelu, J.
Puthucheary, S.D.
Tan, K.L.
author_sort Wong, K.T.
title An immunohistochemical method for the diagnosis of melioidosis
title_short An immunohistochemical method for the diagnosis of melioidosis
title_full An immunohistochemical method for the diagnosis of melioidosis
title_fullStr An immunohistochemical method for the diagnosis of melioidosis
title_full_unstemmed An immunohistochemical method for the diagnosis of melioidosis
title_sort immunohistochemical method for the diagnosis of melioidosis
publisher Faculty of Medicine, University of Malaya
publishDate 1996
url http://eprints.um.edu.my/24486/
https://jummec.um.edu.my/issue/view/661/Vol.1%2C%20No.2%2C%201996
_version_ 1695531084683411456
score 13.211869

Similar Items