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In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2

In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time po...

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Main Authors: Zandi, Keivan, Teoh, Boon Teong, Sam, Sing Sin, Wong, Pooi Fong, Mustafa, Mohd Rais, AbuBakar, Sazaly
Format: Article
Language:English
Published: 2011
Subjects:
R Medicine
SB Plant culture
Online Access:http://eprints.um.edu.my/2412/1/In_vitro_antiviral_activity_of_Fisetin%2C_Rutin_and_Naringenin_against_Dengue_virus_type-2.pdf
http://eprints.um.edu.my/2412/
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spelling my.um.eprints.24122019-12-18T06:32:50Z http://eprints.um.edu.my/2412/ In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2 Zandi, Keivan Teoh, Boon Teong Sam, Sing Sin Wong, Pooi Fong Mustafa, Mohd Rais AbuBakar, Sazaly R Medicine SB Plant culture In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50% cytotoxicity (CC50) values of 247, >1000, and 87 µg/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC50) value of 55 µg/mL and selectivity index (SI) of 4.49. The IC50 value of fisetin was 43.12 µg/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days postinfection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC50 = 52.64 µg/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity. 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2412/1/In_vitro_antiviral_activity_of_Fisetin%2C_Rutin_and_Naringenin_against_Dengue_virus_type-2.pdf Zandi, Keivan and Teoh, Boon Teong and Sam, Sing Sin and Wong, Pooi Fong and Mustafa, Mohd Rais and AbuBakar, Sazaly (2011) In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2. Journal of Medicinal Plants Research, 5 (23). pp. 5534-5539. ISSN 1996-0875
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
SB Plant culture
spellingShingle R Medicine
SB Plant culture
Zandi, Keivan
Teoh, Boon Teong
Sam, Sing Sin
Wong, Pooi Fong
Mustafa, Mohd Rais
AbuBakar, Sazaly
In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
description In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50% cytotoxicity (CC50) values of 247, >1000, and 87 µg/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC50) value of 55 µg/mL and selectivity index (SI) of 4.49. The IC50 value of fisetin was 43.12 µg/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days postinfection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC50 = 52.64 µg/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity.
format Article
author Zandi, Keivan
Teoh, Boon Teong
Sam, Sing Sin
Wong, Pooi Fong
Mustafa, Mohd Rais
AbuBakar, Sazaly
author_facet Zandi, Keivan
Teoh, Boon Teong
Sam, Sing Sin
Wong, Pooi Fong
Mustafa, Mohd Rais
AbuBakar, Sazaly
author_sort Zandi, Keivan
title In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
title_short In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
title_full In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
title_fullStr In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
title_full_unstemmed In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
title_sort in vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2
publishDate 2011
url http://eprints.um.edu.my/2412/1/In_vitro_antiviral_activity_of_Fisetin%2C_Rutin_and_Naringenin_against_Dengue_virus_type-2.pdf
http://eprints.um.edu.my/2412/
_version_ 1654960582789431296
score 13.211869

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