Cover Image

Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress

Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) fro...

Full description

Saved in:
Bibliographic Details
Main Authors: Sultana, Shahnaz, Paul, Sumonta C., Parveen, Samia, Alam, Saiful, Rahman, Naziza, Jannat, Bushra, Hoque, Sirajul, Rahman, Mohammad Tariqur, Karim, Muhammad Manjurul
Format: Article
Language:English
Published: Canadian Science Publishing (NRC Research Press) 2020
Subjects:
QH301 Biology
S Agriculture (General)
Online Access:http://eprints.um.edu.my/24022/1/artilceall.pdf
http://eprints.um.edu.my/24022/
https://www.nrcresearchpress.com/doi/10.1139/cjm-2019-0323
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.24022
record_format eprints
spelling my.um.eprints.240222020-03-12T03:22:01Z http://eprints.um.edu.my/24022/ Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress Sultana, Shahnaz Paul, Sumonta C. Parveen, Samia Alam, Saiful Rahman, Naziza Jannat, Bushra Hoque, Sirajul Rahman, Mohammad Tariqur Karim, Muhammad Manjurul QH301 Biology S Agriculture (General) Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and nonsaline areas in Bangladesh where electrical conductivity was measured as >7.45 and <1.80 dS/m, respectively. Bacteria isolated from saline areas were able to grow in a salt concentration of up to 2.60 mol/L, contrary to the isolates collected from non-saline areas that did not survive beyond 854 mmol/L. Among the salt-tolerant isolates, Bacillus aryabhattai, Achromobacter denitrificans, and Ochrobactrum intermedium, identified by comparing respective sequences of 16S rRNA using the NCBI GenBank, exhibited a higher amount of atmospheric nitrogen fixation, phosphate solubilization, and indoleacetic acid production at 200 mmol/L salt stress. Salt-tolerant isolates exhibited greater resistance to heavy metals and antibiotics, which could be due to the production of an exopolysaccharide layer outside the cell surface. Oryza sativa L. fertilized with B. aryabhattai MS3 and grown under 200 mmol/L salt stress was found to be favoured by enhanced expression of a set of at least four salt-responsive plant genes: BZ8, SOS1, GIG, and NHX1. Fertilization of rice with osmoprotectant-producing PGPR, therefore, could be a climate-change-preparedness strategy for coastal agriculture. Canadian Science Publishing (NRC Research Press) 2020 Article PeerReviewed text en http://eprints.um.edu.my/24022/1/artilceall.pdf Sultana, Shahnaz and Paul, Sumonta C. and Parveen, Samia and Alam, Saiful and Rahman, Naziza and Jannat, Bushra and Hoque, Sirajul and Rahman, Mohammad Tariqur and Karim, Muhammad Manjurul (2020) Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress. Canadian Journal of Microbiology, 66 (2). pp. 144-160. ISSN 0008-4166 https://www.nrcresearchpress.com/doi/10.1139/cjm-2019-0323 doi:10.1139/cjm-2019-0323
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QH301 Biology
S Agriculture (General)
spellingShingle QH301 Biology
S Agriculture (General)
Sultana, Shahnaz
Paul, Sumonta C.
Parveen, Samia
Alam, Saiful
Rahman, Naziza
Jannat, Bushra
Hoque, Sirajul
Rahman, Mohammad Tariqur
Karim, Muhammad Manjurul
Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
description Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and nonsaline areas in Bangladesh where electrical conductivity was measured as >7.45 and <1.80 dS/m, respectively. Bacteria isolated from saline areas were able to grow in a salt concentration of up to 2.60 mol/L, contrary to the isolates collected from non-saline areas that did not survive beyond 854 mmol/L. Among the salt-tolerant isolates, Bacillus aryabhattai, Achromobacter denitrificans, and Ochrobactrum intermedium, identified by comparing respective sequences of 16S rRNA using the NCBI GenBank, exhibited a higher amount of atmospheric nitrogen fixation, phosphate solubilization, and indoleacetic acid production at 200 mmol/L salt stress. Salt-tolerant isolates exhibited greater resistance to heavy metals and antibiotics, which could be due to the production of an exopolysaccharide layer outside the cell surface. Oryza sativa L. fertilized with B. aryabhattai MS3 and grown under 200 mmol/L salt stress was found to be favoured by enhanced expression of a set of at least four salt-responsive plant genes: BZ8, SOS1, GIG, and NHX1. Fertilization of rice with osmoprotectant-producing PGPR, therefore, could be a climate-change-preparedness strategy for coastal agriculture.
format Article
author Sultana, Shahnaz
Paul, Sumonta C.
Parveen, Samia
Alam, Saiful
Rahman, Naziza
Jannat, Bushra
Hoque, Sirajul
Rahman, Mohammad Tariqur
Karim, Muhammad Manjurul
author_facet Sultana, Shahnaz
Paul, Sumonta C.
Parveen, Samia
Alam, Saiful
Rahman, Naziza
Jannat, Bushra
Hoque, Sirajul
Rahman, Mohammad Tariqur
Karim, Muhammad Manjurul
author_sort Sultana, Shahnaz
title Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
title_short Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
title_full Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
title_fullStr Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
title_full_unstemmed Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
title_sort isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
publisher Canadian Science Publishing (NRC Research Press)
publishDate 2020
url http://eprints.um.edu.my/24022/1/artilceall.pdf
http://eprints.um.edu.my/24022/
https://www.nrcresearchpress.com/doi/10.1139/cjm-2019-0323
_version_ 1662755212264210432
score 13.211869

Similar Items