Staff View: Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates pr...

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Main Authors:	Quah, Yixian, Mohd Ismail, Nor Ismaliza, Ooi, Jillian Lean Sim, Affendi, Yang Amri, Abd Manan, Fazilah, Teh, Lai Kuan, Wong, Fai Chu, Chai, Tsun Thai
Format:	Article
Published:	Springer Verlag 2019
Subjects:	G Geography. Anthropology. Recreation QR Microbiology R Medicine SH Aquaculture. Fisheries. Angling
Online Access:	http://eprints.um.edu.my/23723/ https://doi.org/10.1631/jzus.B1700586
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spelling	my.um.eprints.237232020-02-10T08:10:44Z http://eprints.um.edu.my/23723/ Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum Quah, Yixian Mohd Ismail, Nor Ismaliza Ooi, Jillian Lean Sim Affendi, Yang Amri Abd Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai G Geography. Anthropology. Recreation QR Microbiology R Medicine SH Aquaculture. Fisheries. Angling Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50 ) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs. © 2019, Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature. Springer Verlag 2019 Article PeerReviewed Quah, Yixian and Mohd Ismail, Nor Ismaliza and Ooi, Jillian Lean Sim and Affendi, Yang Amri and Abd Manan, Fazilah and Teh, Lai Kuan and Wong, Fai Chu and Chai, Tsun Thai (2019) Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum. Journal of Zhejiang University-SCIENCE B, 20 (1). pp. 59-70. ISSN 1673-1581 https://doi.org/10.1631/jzus.B1700586 doi:10.1631/jzus.B1700586
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	G Geography. Anthropology. Recreation QR Microbiology R Medicine SH Aquaculture. Fisheries. Angling
spellingShingle	G Geography. Anthropology. Recreation QR Microbiology R Medicine SH Aquaculture. Fisheries. Angling Quah, Yixian Mohd Ismail, Nor Ismaliza Ooi, Jillian Lean Sim Affendi, Yang Amri Abd Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
description	Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50 ) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs. © 2019, Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature.
format	Article
author	Quah, Yixian Mohd Ismail, Nor Ismaliza Ooi, Jillian Lean Sim Affendi, Yang Amri Abd Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai
author_facet	Quah, Yixian Mohd Ismail, Nor Ismaliza Ooi, Jillian Lean Sim Affendi, Yang Amri Abd Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai
author_sort	Quah, Yixian
title	Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
title_short	Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
title_full	Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
title_fullStr	Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
title_full_unstemmed	Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
title_sort	purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum
publisher	Springer Verlag
publishDate	2019
url	http://eprints.um.edu.my/23723/ https://doi.org/10.1631/jzus.B1700586
_version_	1662755170758426624
score	13.211869

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

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