Staff View: Carnosine exhibits significant antiviral activity against Dengue and Zika virus

Carnosine exhibits significant antiviral activity against Dengue and Zika virus

Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses transmitted to humans by their common vector, Aedes mosquitoes. DENV infection represents one of the most widely spread mosquito-borne diseases whereas ZIKV infection occasionally re-emerged in the past causing outbreaks. Although there have...

Full description

Saved in:

Bibliographic Details
Main Authors:	Rothan, Hussin A., Abdulrahman, Ammar Yasir, Khazali, Ahmad Suhail, Rashid, Nurshamimi Nor, Teoh, Teow Chong, Yusof, Rohana
Format:	Article
Published:	Wiley 2019
Subjects:	QR Microbiology R Medicine
Online Access:	http://eprints.um.edu.my/23624/ https://doi.org/10.1002/psc.3196
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.23624
record_format	eprints
spelling	my.um.eprints.236242020-01-29T04:27:43Z http://eprints.um.edu.my/23624/ Carnosine exhibits significant antiviral activity against Dengue and Zika virus Rothan, Hussin A. Abdulrahman, Ammar Yasir Khazali, Ahmad Suhail Rashid, Nurshamimi Nor Teoh, Teow Chong Yusof, Rohana QR Microbiology R Medicine Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses transmitted to humans by their common vector, Aedes mosquitoes. DENV infection represents one of the most widely spread mosquito-borne diseases whereas ZIKV infection occasionally re-emerged in the past causing outbreaks. Although there have been considerable advances in understanding the pathophysiology of these viruses, no effective vaccines or antiviral drugs are currently available. In this study, we evaluated the antiviral activity of carnosine, an endogenous dipeptide (β-alanyl-l-histidine), against DENV serotype 2 (DENV2) and ZIKV infection in human liver cells (Huh7). Computational studies were performed to predict the potential interactions between carnosine and viral proteins. Biochemical and cell-based assays were performed to validate the computational results. Mode-of-inhibition, plaque reduction, and immunostaining assays were performed to determine the antiviral activity of carnosine. Exogenous carnosine showed minimal cytotoxicity in Huh7 cells and rescued the viability of infected cells with EC50 values of 52.3 and 59.5 μM for DENV2 and ZIKV infection, respectively. Based on the mode-of-inhibition assays, carnosine inhibited DENV2 mainly by inhibiting viral genome replication and interfering with virus entry. Carnosine antiviral activity was verified with immunostaining assay where carnosine treatment diminished viral fluorescence signal. In conclusion, carnosine exhibited significant inhibitory effects against DENV2 and ZIKV replication in human liver cells and could be utilized as a lead peptide for the development of effective and safe antiviral agents against DENV and ZIKV. © 2019 European Peptide Society and John Wiley & Sons, Ltd. Wiley 2019 Article PeerReviewed Rothan, Hussin A. and Abdulrahman, Ammar Yasir and Khazali, Ahmad Suhail and Rashid, Nurshamimi Nor and Teoh, Teow Chong and Yusof, Rohana (2019) Carnosine exhibits significant antiviral activity against Dengue and Zika virus. Journal of Peptide Science, 25 (8). e3196. ISSN 1075-2617 https://doi.org/10.1002/psc.3196 doi:10.1002/psc.3196
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QR Microbiology R Medicine
spellingShingle	QR Microbiology R Medicine Rothan, Hussin A. Abdulrahman, Ammar Yasir Khazali, Ahmad Suhail Rashid, Nurshamimi Nor Teoh, Teow Chong Yusof, Rohana Carnosine exhibits significant antiviral activity against Dengue and Zika virus
description	Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses transmitted to humans by their common vector, Aedes mosquitoes. DENV infection represents one of the most widely spread mosquito-borne diseases whereas ZIKV infection occasionally re-emerged in the past causing outbreaks. Although there have been considerable advances in understanding the pathophysiology of these viruses, no effective vaccines or antiviral drugs are currently available. In this study, we evaluated the antiviral activity of carnosine, an endogenous dipeptide (β-alanyl-l-histidine), against DENV serotype 2 (DENV2) and ZIKV infection in human liver cells (Huh7). Computational studies were performed to predict the potential interactions between carnosine and viral proteins. Biochemical and cell-based assays were performed to validate the computational results. Mode-of-inhibition, plaque reduction, and immunostaining assays were performed to determine the antiviral activity of carnosine. Exogenous carnosine showed minimal cytotoxicity in Huh7 cells and rescued the viability of infected cells with EC50 values of 52.3 and 59.5 μM for DENV2 and ZIKV infection, respectively. Based on the mode-of-inhibition assays, carnosine inhibited DENV2 mainly by inhibiting viral genome replication and interfering with virus entry. Carnosine antiviral activity was verified with immunostaining assay where carnosine treatment diminished viral fluorescence signal. In conclusion, carnosine exhibited significant inhibitory effects against DENV2 and ZIKV replication in human liver cells and could be utilized as a lead peptide for the development of effective and safe antiviral agents against DENV and ZIKV. © 2019 European Peptide Society and John Wiley & Sons, Ltd.
format	Article
author	Rothan, Hussin A. Abdulrahman, Ammar Yasir Khazali, Ahmad Suhail Rashid, Nurshamimi Nor Teoh, Teow Chong Yusof, Rohana
author_facet	Rothan, Hussin A. Abdulrahman, Ammar Yasir Khazali, Ahmad Suhail Rashid, Nurshamimi Nor Teoh, Teow Chong Yusof, Rohana
author_sort	Rothan, Hussin A.
title	Carnosine exhibits significant antiviral activity against Dengue and Zika virus
title_short	Carnosine exhibits significant antiviral activity against Dengue and Zika virus
title_full	Carnosine exhibits significant antiviral activity against Dengue and Zika virus
title_fullStr	Carnosine exhibits significant antiviral activity against Dengue and Zika virus
title_full_unstemmed	Carnosine exhibits significant antiviral activity against Dengue and Zika virus
title_sort	carnosine exhibits significant antiviral activity against dengue and zika virus
publisher	Wiley
publishDate	2019
url	http://eprints.um.edu.my/23624/ https://doi.org/10.1002/psc.3196
_version_	1657488237479854080
score	13.211869

Carnosine exhibits significant antiviral activity against Dengue and Zika virus

Similar Items