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Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns

This study evaluates the performance of different detection protocols of Vibrio parahaemolyticus in frozen prawns. The effect of enrichment and incubation temperature on the selectivity of thiosulfate citrate bile sucrose (TCBS) and CHROMagar™ Vibrio (CAV) media were determined. Enumeration of V. pa...

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Main Authors: Sadeghi, Salima, Thong, Kwai Lin, Chai, Lay Ching
Format: Article
Published: Springer International Publishing 2019
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://eprints.um.edu.my/23606/
https://doi.org/10.1007/s00003-019-01237-1
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spelling my.um.eprints.236062020-01-29T00:32:53Z http://eprints.um.edu.my/23606/ Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns Sadeghi, Salima Thong, Kwai Lin Chai, Lay Ching Q Science (General) QR Microbiology This study evaluates the performance of different detection protocols of Vibrio parahaemolyticus in frozen prawns. The effect of enrichment and incubation temperature on the selectivity of thiosulfate citrate bile sucrose (TCBS) and CHROMagar™ Vibrio (CAV) media were determined. Enumeration of V. parahaemolyticus in frozen prawns by direct-plating on CAV at 37 °C yielded the highest count compared to the other tested direct-plating protocols (p < 0.05). The enrichment step was found to increase the selectivity of TCBS for V. parahaemolyticus but did not affect the selectivity of CAV. Furthermore, trh + V. parahaemolyticus isolates were only successfully recovered from CAV, suggesting that CAV is the better media for the detection of virulent V. parahaemolyticus compared to TCBS. Repetitive Extragenic Palindromic PCR typing (REP-PCR) indicated that CAV and TCBS, as well as direct-plating or enrichment-plating are selective for different subsets of V. parahaemolyticus present in a sample. In conclusion, the current work suggests that CAV is a better selective media for detection and isolation of V. parahaemolyticus in frozen prawns. This study also suggests that direct-plating on CAV with an incubation temperature of 35–37 °C could be an alternative to Most Probable Number (MPN)-PCR for enumeration of V. parahaemolyticus in frozen prawn samples. © 2019, Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL). Springer International Publishing 2019 Article PeerReviewed Sadeghi, Salima and Thong, Kwai Lin and Chai, Lay Ching (2019) Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns. Journal of Consumer Protection and Food Safety, 14 (4). pp. 355-364. ISSN 1661-5751 https://doi.org/10.1007/s00003-019-01237-1 doi:10.1007/s00003-019-01237-1
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Sadeghi, Salima
Thong, Kwai Lin
Chai, Lay Ching
Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
description This study evaluates the performance of different detection protocols of Vibrio parahaemolyticus in frozen prawns. The effect of enrichment and incubation temperature on the selectivity of thiosulfate citrate bile sucrose (TCBS) and CHROMagar™ Vibrio (CAV) media were determined. Enumeration of V. parahaemolyticus in frozen prawns by direct-plating on CAV at 37 °C yielded the highest count compared to the other tested direct-plating protocols (p < 0.05). The enrichment step was found to increase the selectivity of TCBS for V. parahaemolyticus but did not affect the selectivity of CAV. Furthermore, trh + V. parahaemolyticus isolates were only successfully recovered from CAV, suggesting that CAV is the better media for the detection of virulent V. parahaemolyticus compared to TCBS. Repetitive Extragenic Palindromic PCR typing (REP-PCR) indicated that CAV and TCBS, as well as direct-plating or enrichment-plating are selective for different subsets of V. parahaemolyticus present in a sample. In conclusion, the current work suggests that CAV is a better selective media for detection and isolation of V. parahaemolyticus in frozen prawns. This study also suggests that direct-plating on CAV with an incubation temperature of 35–37 °C could be an alternative to Most Probable Number (MPN)-PCR for enumeration of V. parahaemolyticus in frozen prawn samples. © 2019, Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL).
format Article
author Sadeghi, Salima
Thong, Kwai Lin
Chai, Lay Ching
author_facet Sadeghi, Salima
Thong, Kwai Lin
Chai, Lay Ching
author_sort Sadeghi, Salima
title Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
title_short Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
title_full Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
title_fullStr Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
title_full_unstemmed Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns
title_sort pre-enrichment step, incubation temperature and type of selective media affect the pathogenic vibrio parahaemolyticus detection efficiency in frozen prawns
publisher Springer International Publishing
publishDate 2019
url http://eprints.um.edu.my/23606/
https://doi.org/10.1007/s00003-019-01237-1
_version_ 1657488234581590016
score 13.211869

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