Staff View: Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes

Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes

The cell wall determines morphology and the environmental responses of plant cells. The primary cell wall (PCW) is produced during cell division and expansion, determining the cell shape and volume. After cell expansion, specific types of plant cells produce a lignified wall, known as a secondary ce...

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Main Authors:	Saelim, Laddawan, Akiyoshi, Nobuhiro, Tan, Tian Tian, Ihara, Ayumi, Yamaguchi, Masatoshi, Hirano, Ko, Matsuoka, Makoto, Demura, Taku, Ohtani, Misato
Format:	Article
Published:	Springer Verlag 2019
Subjects:	Q Science (General) QH Natural history T Technology (General)
Online Access:	http://eprints.um.edu.my/23599/ https://doi.org/10.1007/s10265-018-1074-1
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spelling	my.um.eprints.235992020-01-28T02:30:25Z http://eprints.um.edu.my/23599/ Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes Saelim, Laddawan Akiyoshi, Nobuhiro Tan, Tian Tian Ihara, Ayumi Yamaguchi, Masatoshi Hirano, Ko Matsuoka, Makoto Demura, Taku Ohtani, Misato Q Science (General) QH Natural history T Technology (General) The cell wall determines morphology and the environmental responses of plant cells. The primary cell wall (PCW) is produced during cell division and expansion, determining the cell shape and volume. After cell expansion, specific types of plant cells produce a lignified wall, known as a secondary cell wall (SCW). We functionally analyzed Group IIId Arabidopsis AP2/EREBP genes, namely ERF34, ERF35, ERF38, and ERF39, which are homologs of a rice ERF gene previously proposed to be related to SCW biosynthesis. Expression analysis revealed that these four genes are expressed in regions related to cell division and/or cell differentiation in seedlings (i.e., shoot apical meristems, the primordia of leaves and lateral roots, trichomes, and central cylinder of primary roots) and flowers (i.e., vascular tissues of floral organs and replums and/or valve margins of pistils). Overexpression of ERF genes significantly upregulated PCW-type, but not SCW-type, CESA genes encoding cellulose synthase catalytic subunits in Arabidopsis seedlings. Transient co-expression reporter analysis indicated that ERF35, ERF38, and ERF39 possess transcriptional activator activity, and that ERF34, ERF35, ERF38, and ERF39 upregulated the promoter activity of CESA1, a PCW-type CESA gene, through the DRECRTCOREAT elements, the core cis-acting elements known to be recognized by AP2/ERF proteins. Together, our findings show that Group IIId ERF genes are positive transcriptional regulators of PCW-type CESA genes in Arabidopsis and are possibly involved in modulating cellulose biosynthesis in response to developmental requirements and environmental stimuli. © 2018, The Botanical Society of Japan and Springer Japan KK, part of Springer Nature. Springer Verlag 2019 Article PeerReviewed Saelim, Laddawan and Akiyoshi, Nobuhiro and Tan, Tian Tian and Ihara, Ayumi and Yamaguchi, Masatoshi and Hirano, Ko and Matsuoka, Makoto and Demura, Taku and Ohtani, Misato (2019) Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes. Journal of Plant Research, 132 (1). pp. 117-129. ISSN 0918-9440 https://doi.org/10.1007/s10265-018-1074-1 doi:10.1007/s10265-018-1074-1
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH Natural history T Technology (General)
spellingShingle	Q Science (General) QH Natural history T Technology (General) Saelim, Laddawan Akiyoshi, Nobuhiro Tan, Tian Tian Ihara, Ayumi Yamaguchi, Masatoshi Hirano, Ko Matsuoka, Makoto Demura, Taku Ohtani, Misato Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
description	The cell wall determines morphology and the environmental responses of plant cells. The primary cell wall (PCW) is produced during cell division and expansion, determining the cell shape and volume. After cell expansion, specific types of plant cells produce a lignified wall, known as a secondary cell wall (SCW). We functionally analyzed Group IIId Arabidopsis AP2/EREBP genes, namely ERF34, ERF35, ERF38, and ERF39, which are homologs of a rice ERF gene previously proposed to be related to SCW biosynthesis. Expression analysis revealed that these four genes are expressed in regions related to cell division and/or cell differentiation in seedlings (i.e., shoot apical meristems, the primordia of leaves and lateral roots, trichomes, and central cylinder of primary roots) and flowers (i.e., vascular tissues of floral organs and replums and/or valve margins of pistils). Overexpression of ERF genes significantly upregulated PCW-type, but not SCW-type, CESA genes encoding cellulose synthase catalytic subunits in Arabidopsis seedlings. Transient co-expression reporter analysis indicated that ERF35, ERF38, and ERF39 possess transcriptional activator activity, and that ERF34, ERF35, ERF38, and ERF39 upregulated the promoter activity of CESA1, a PCW-type CESA gene, through the DRECRTCOREAT elements, the core cis-acting elements known to be recognized by AP2/ERF proteins. Together, our findings show that Group IIId ERF genes are positive transcriptional regulators of PCW-type CESA genes in Arabidopsis and are possibly involved in modulating cellulose biosynthesis in response to developmental requirements and environmental stimuli. © 2018, The Botanical Society of Japan and Springer Japan KK, part of Springer Nature.
format	Article
author	Saelim, Laddawan Akiyoshi, Nobuhiro Tan, Tian Tian Ihara, Ayumi Yamaguchi, Masatoshi Hirano, Ko Matsuoka, Makoto Demura, Taku Ohtani, Misato
author_facet	Saelim, Laddawan Akiyoshi, Nobuhiro Tan, Tian Tian Ihara, Ayumi Yamaguchi, Masatoshi Hirano, Ko Matsuoka, Makoto Demura, Taku Ohtani, Misato
author_sort	Saelim, Laddawan
title	Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
title_short	Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
title_full	Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
title_fullStr	Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
title_full_unstemmed	Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
title_sort	arabidopsis group iiid erf proteins positively regulate primary cell wall-type cesa genes
publisher	Springer Verlag
publishDate	2019
url	http://eprints.um.edu.my/23599/ https://doi.org/10.1007/s10265-018-1074-1
_version_	1657488233448079360
score	13.211869

Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes

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