Staff View: In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats

In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats

Background: The effect of delivered bromine vapor and also bromine substitutions are shown to play an important role in anti-inflammatory activity. The present study encompasses a broad in vivo study to size up healing activity of a novel dibromide substituted Schiff based compound against cutaneous...

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Main Authors:	Saremi, Kamelia, Bagheri, Elham, Rad, Sima Kianpour, Salman, Abbas Abdulameer, Majid, Nazia Abdul
Format:	Article
Published:	Elsevier 2019
Subjects:	Q Science (General) QD Chemistry QH Natural history QR Microbiology R Medicine
Online Access:	http://eprints.um.edu.my/23527/ https://doi.org/10.1016/j.genrep.2019.100429
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spelling	my.um.eprints.235272020-01-22T02:40:06Z http://eprints.um.edu.my/23527/ In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats Saremi, Kamelia Bagheri, Elham Rad, Sima Kianpour Salman, Abbas Abdulameer Majid, Nazia Abdul Q Science (General) QD Chemistry QH Natural history QR Microbiology R Medicine Background: The effect of delivered bromine vapor and also bromine substitutions are shown to play an important role in anti-inflammatory activity. The present study encompasses a broad in vivo study to size up healing activity of a novel dibromide substituted Schiff based compound against cutaneous wound model in Sprague Dawley (SD) rats. Methodology/principal findings: 2, 2′-[1, 2-Cyclohexanediylbis(nitriloethylidyne)] bis[4-bromophenol], CNBP, is synthesized through a Schiff base reaction applying the related ketone and diamine as the initiators. Four groups of six in each male SD rats are divided as negative group which are treated with gum acacia, positive control animals which are treated with topical dosage of Intrasite gel, and testing groups treated with low (10 mg/kg) and high (20 mg/kg) doses of CNBP. The excisional wounds are created on the posterior neck area of each group and the wound closure percentage was measured in the two separated days of the experiment. Moreover, the histopathological evaluation and determination of activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (GPx), and malondialdehyde (MDA) of the skin sections are performed. Furthermore, the immunohistochemistry consists of the evaluation of Hsp70 and BAX proteins. Conclusions/significance: The wound closure percentage showed a significant increase in high dose CNBP-treated group compared to the negative control. Histopathological evaluation of the skin sections showed that granulation tissue contained more proliferating fibroblast, collagen deposition, angiogenesis, and also less inflammatory cells in the high dose CNBP-treated group compared to the normal rats. In the treated groups with CNBP, SOD, CAT, and GPx activities were found significantly higher, however, the MDA level was shown to be lower (P < 0.05; P < 0.01; **P < 0.001) than the negative control. At the molecular level, CNBP (20 mg/ml, HD) improved wound-healing process via down and up regulation of Bax and Hsp70 protein, respectively at the wound site. © 2019 Elsevier 2019 Article PeerReviewed Saremi, Kamelia and Bagheri, Elham and Rad, Sima Kianpour and Salman, Abbas Abdulameer and Majid, Nazia Abdul (2019) In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats. Gene Reports, 16. p. 100429. ISSN 2452-0144 https://doi.org/10.1016/j.genrep.2019.100429 doi:10.1016/j.genrep.2019.100429
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QD Chemistry QH Natural history QR Microbiology R Medicine
spellingShingle	Q Science (General) QD Chemistry QH Natural history QR Microbiology R Medicine Saremi, Kamelia Bagheri, Elham Rad, Sima Kianpour Salman, Abbas Abdulameer Majid, Nazia Abdul In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
description	Background: The effect of delivered bromine vapor and also bromine substitutions are shown to play an important role in anti-inflammatory activity. The present study encompasses a broad in vivo study to size up healing activity of a novel dibromide substituted Schiff based compound against cutaneous wound model in Sprague Dawley (SD) rats. Methodology/principal findings: 2, 2′-[1, 2-Cyclohexanediylbis(nitriloethylidyne)] bis[4-bromophenol], CNBP, is synthesized through a Schiff base reaction applying the related ketone and diamine as the initiators. Four groups of six in each male SD rats are divided as negative group which are treated with gum acacia, positive control animals which are treated with topical dosage of Intrasite gel, and testing groups treated with low (10 mg/kg) and high (20 mg/kg) doses of CNBP. The excisional wounds are created on the posterior neck area of each group and the wound closure percentage was measured in the two separated days of the experiment. Moreover, the histopathological evaluation and determination of activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (GPx), and malondialdehyde (MDA) of the skin sections are performed. Furthermore, the immunohistochemistry consists of the evaluation of Hsp70 and BAX proteins. Conclusions/significance: The wound closure percentage showed a significant increase in high dose CNBP-treated group compared to the negative control. Histopathological evaluation of the skin sections showed that granulation tissue contained more proliferating fibroblast, collagen deposition, angiogenesis, and also less inflammatory cells in the high dose CNBP-treated group compared to the normal rats. In the treated groups with CNBP, SOD, CAT, and GPx activities were found significantly higher, however, the MDA level was shown to be lower (P < 0.05; P < 0.01; **P < 0.001) than the negative control. At the molecular level, CNBP (20 mg/ml, HD) improved wound-healing process via down and up regulation of Bax and Hsp70 protein, respectively at the wound site. © 2019
format	Article
author	Saremi, Kamelia Bagheri, Elham Rad, Sima Kianpour Salman, Abbas Abdulameer Majid, Nazia Abdul
author_facet	Saremi, Kamelia Bagheri, Elham Rad, Sima Kianpour Salman, Abbas Abdulameer Majid, Nazia Abdul
author_sort	Saremi, Kamelia
title	In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
title_short	In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
title_full	In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
title_fullStr	In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
title_full_unstemmed	In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats
title_sort	in vivo evaluation of wound healing improvement of a new schiff base derived bromine compound (cnbp) in rats
publisher	Elsevier
publishDate	2019
url	http://eprints.um.edu.my/23527/ https://doi.org/10.1016/j.genrep.2019.100429
_version_	1657488222854316032
score	13.211869

In vivo evaluation of wound healing improvement of a new Schiff base derived bromine compound (CNBP) in rats

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