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Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice

Ethnopharmacological relevance: Cynometra cauliflora Linn. belongs to the Fabaceae family and is known locally in Malaysia as nam-nam. Traditionally, a decoction of the C. cauliflora leaves is used for treating hyperlipidemia and diabetes. Aim of the study: This study aims to investigate the anti-ob...

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Main Authors: Seyedan, Atefehalsadat, Mohamed, Zahurin, Alshagga, Mustafa Ahmed, Koosha, Sanaz, Alshawsh, Mohammed Abdullah
Format: Article
Published: Elsevier 2019
Subjects:
R Medicine
RM Therapeutics. Pharmacology
Online Access:http://eprints.um.edu.my/23495/
https://doi.org/10.1016/j.jep.2019.03.001
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spelling my.um.eprints.234952020-01-21T05:10:43Z http://eprints.um.edu.my/23495/ Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice Seyedan, Atefehalsadat Mohamed, Zahurin Alshagga, Mustafa Ahmed Koosha, Sanaz Alshawsh, Mohammed Abdullah R Medicine RM Therapeutics. Pharmacology Ethnopharmacological relevance: Cynometra cauliflora Linn. belongs to the Fabaceae family and is known locally in Malaysia as nam-nam. Traditionally, a decoction of the C. cauliflora leaves is used for treating hyperlipidemia and diabetes. Aim of the study: This study aims to investigate the anti-obesity and lipid lowering effects of ethanolic extract of C. cauliflora leaves and its major compound (vitexin) in C57BL/6 obese mice induced by high-fat diet (HFD), as well as to further identify the molecular mechanism underlying this action. Methods and material: Male C57BL/6 mice were fed with HFD (60% fat) for 16 weeks to become obese. The treatment started during the last 8 weeks of HFD feeding and the obese mice were treated with C. cauliflora leaf extract at 200 and 400 mg/kg/day, orlistat (10 mg/kg) and vitexin (10 mg/kg). Results: The oral administration of C. cauliflora (400 and 200 mg/kg) and vitexin significantly reduced body weight, adipose tissue and liver weight and lipid accumulation in the liver compared to control HFD group. Both doses of C. cauliflora also significantly (P ≤ 0.05) decreased serum triglyceride, LDL, lipase, IL-6, peptide YY, resistin levels, hyperglycemia, hyperinsulinemia, and hyperleptinemia compared to the control HFD group. Moreover, C. cauliflora significantly up-regulated the expression of adiponectin, Glut4, Mtor, IRS-1 and InsR genes, and significantly decreased the expression of Lepr in white adipose tissue. Furthermore, C. cauliflora significantly up-regulated the expression of hypothalamus Glut4, Mtor and NF-kB genes. GC-MS analysis of C. cauliflora leaves detected the presence of phytol, vitamin E and β-sitosterol. Besides, the phytochemical evaluation of C. cauliflora leaves showed the presence of flavonoid, saponin and phenolic compounds. Conclusion: This study shows interesting outcomes of C. cauliflora against HFD-induced obesity and associated metabolic abnormalities. Therefore, the C. cauliflora extract could be a potentially effective agent for obesity management and its related metabolic disorders such as insulin resistance and hyperlipidemia. © 2019 Elsevier B.V. Elsevier 2019 Article PeerReviewed Seyedan, Atefehalsadat and Mohamed, Zahurin and Alshagga, Mustafa Ahmed and Koosha, Sanaz and Alshawsh, Mohammed Abdullah (2019) Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice. Journal of Ethnopharmacology, 236. pp. 173-182. ISSN 0378-8741 https://doi.org/10.1016/j.jep.2019.03.001 doi:10.1016/j.jep.2019.03.001
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
RM Therapeutics. Pharmacology
spellingShingle R Medicine
RM Therapeutics. Pharmacology
Seyedan, Atefehalsadat
Mohamed, Zahurin
Alshagga, Mustafa Ahmed
Koosha, Sanaz
Alshawsh, Mohammed Abdullah
Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
description Ethnopharmacological relevance: Cynometra cauliflora Linn. belongs to the Fabaceae family and is known locally in Malaysia as nam-nam. Traditionally, a decoction of the C. cauliflora leaves is used for treating hyperlipidemia and diabetes. Aim of the study: This study aims to investigate the anti-obesity and lipid lowering effects of ethanolic extract of C. cauliflora leaves and its major compound (vitexin) in C57BL/6 obese mice induced by high-fat diet (HFD), as well as to further identify the molecular mechanism underlying this action. Methods and material: Male C57BL/6 mice were fed with HFD (60% fat) for 16 weeks to become obese. The treatment started during the last 8 weeks of HFD feeding and the obese mice were treated with C. cauliflora leaf extract at 200 and 400 mg/kg/day, orlistat (10 mg/kg) and vitexin (10 mg/kg). Results: The oral administration of C. cauliflora (400 and 200 mg/kg) and vitexin significantly reduced body weight, adipose tissue and liver weight and lipid accumulation in the liver compared to control HFD group. Both doses of C. cauliflora also significantly (P ≤ 0.05) decreased serum triglyceride, LDL, lipase, IL-6, peptide YY, resistin levels, hyperglycemia, hyperinsulinemia, and hyperleptinemia compared to the control HFD group. Moreover, C. cauliflora significantly up-regulated the expression of adiponectin, Glut4, Mtor, IRS-1 and InsR genes, and significantly decreased the expression of Lepr in white adipose tissue. Furthermore, C. cauliflora significantly up-regulated the expression of hypothalamus Glut4, Mtor and NF-kB genes. GC-MS analysis of C. cauliflora leaves detected the presence of phytol, vitamin E and β-sitosterol. Besides, the phytochemical evaluation of C. cauliflora leaves showed the presence of flavonoid, saponin and phenolic compounds. Conclusion: This study shows interesting outcomes of C. cauliflora against HFD-induced obesity and associated metabolic abnormalities. Therefore, the C. cauliflora extract could be a potentially effective agent for obesity management and its related metabolic disorders such as insulin resistance and hyperlipidemia. © 2019 Elsevier B.V.
format Article
author Seyedan, Atefehalsadat
Mohamed, Zahurin
Alshagga, Mustafa Ahmed
Koosha, Sanaz
Alshawsh, Mohammed Abdullah
author_facet Seyedan, Atefehalsadat
Mohamed, Zahurin
Alshagga, Mustafa Ahmed
Koosha, Sanaz
Alshawsh, Mohammed Abdullah
author_sort Seyedan, Atefehalsadat
title Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
title_short Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
title_full Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
title_fullStr Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
title_full_unstemmed Cynometra cauliflora Linn. Attenuates metabolic abnormalities in high-fat diet-induced obese mice
title_sort cynometra cauliflora linn. attenuates metabolic abnormalities in high-fat diet-induced obese mice
publisher Elsevier
publishDate 2019
url http://eprints.um.edu.my/23495/
https://doi.org/10.1016/j.jep.2019.03.001
_version_ 1657488217886162944
score 13.211869

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