Staff View: A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma

A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which is highly associated with Epstein–Barr virus (EBV). Worldwide, most of the top 20 countries with the highest incidence and mortality rates of NPC are low- and middle-income countries. Many studies had demonstrated that E...

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Main Authors:	Tan, Geok Wee, Sivanesan, Vijaya Mohan, Abdul Rahman, Farah Ida, Hassan, Faridah, Hasbullah, Harissa Husainy, Ng, Ching Ching, Khoo, Alan Soo Beng, Tan, Lu Ping
Format:	Article
Published:	John Wiley & Sons 2019
Subjects:	Q Science (General) QH Natural history QR Microbiology R Medicine
Online Access:	http://eprints.um.edu.my/23309/ https://doi.org/10.1002/ijc.32173
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spelling	my.um.eprints.233092020-01-06T02:14:33Z http://eprints.um.edu.my/23309/ A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma Tan, Geok Wee Sivanesan, Vijaya Mohan Abdul Rahman, Farah Ida Hassan, Faridah Hasbullah, Harissa Husainy Ng, Ching Ching Khoo, Alan Soo Beng Tan, Lu Ping Q Science (General) QH Natural history QR Microbiology R Medicine Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which is highly associated with Epstein–Barr virus (EBV). Worldwide, most of the top 20 countries with the highest incidence and mortality rates of NPC are low- and middle-income countries. Many studies had demonstrated that EBV could be detected in the tissue, serum and plasma of NPC patients. In this study, we explored the potential of assays based on non-invasive nasal washings (NW) as a diagnostic and prognostic tool for NPC. A total of 128 patients were evaluated for NW EBV DNA loads and a subset of these samples were also tested for 27 EBV and human miRNAs shortlisted from literature. EBV DNA and seven miRNAs showed area under the receiver operating characteristic curve (AUC) values of more than 0.7, suggestive of their potential utility to detect NPC. Logistic regression analyses suggested that combination of two NW assays that test for EBNA-1 and hsa-miR-21 had the best performance in detecting NPC. The trend of NW EBV DNA load matched with clinical outcome of 71.4% (10 out of 14) NPC patients being followed-up. In summary, the non-invasive NW testing panel may be particularly useful for NPC screening in remote areas where healthcare facilities and otolaryngologists are lacking, and may encourage frequent testing of individuals in the high risk groups who are reluctant to have their blood tested. However, further validation in an independent cohort is required to strengthen the utility of this testing panel as a non-invasive detection tool for NPC. © 2019 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC John Wiley & Sons 2019 Article PeerReviewed Tan, Geok Wee and Sivanesan, Vijaya Mohan and Abdul Rahman, Farah Ida and Hassan, Faridah and Hasbullah, Harissa Husainy and Ng, Ching Ching and Khoo, Alan Soo Beng and Tan, Lu Ping (2019) A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma. International Journal of Cancer, 145 (8). pp. 2260-2266. ISSN 0020-7136 https://doi.org/10.1002/ijc.32173 doi:10.1002/ijc.32173
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH Natural history QR Microbiology R Medicine
spellingShingle	Q Science (General) QH Natural history QR Microbiology R Medicine Tan, Geok Wee Sivanesan, Vijaya Mohan Abdul Rahman, Farah Ida Hassan, Faridah Hasbullah, Harissa Husainy Ng, Ching Ching Khoo, Alan Soo Beng Tan, Lu Ping A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
description	Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which is highly associated with Epstein–Barr virus (EBV). Worldwide, most of the top 20 countries with the highest incidence and mortality rates of NPC are low- and middle-income countries. Many studies had demonstrated that EBV could be detected in the tissue, serum and plasma of NPC patients. In this study, we explored the potential of assays based on non-invasive nasal washings (NW) as a diagnostic and prognostic tool for NPC. A total of 128 patients were evaluated for NW EBV DNA loads and a subset of these samples were also tested for 27 EBV and human miRNAs shortlisted from literature. EBV DNA and seven miRNAs showed area under the receiver operating characteristic curve (AUC) values of more than 0.7, suggestive of their potential utility to detect NPC. Logistic regression analyses suggested that combination of two NW assays that test for EBNA-1 and hsa-miR-21 had the best performance in detecting NPC. The trend of NW EBV DNA load matched with clinical outcome of 71.4% (10 out of 14) NPC patients being followed-up. In summary, the non-invasive NW testing panel may be particularly useful for NPC screening in remote areas where healthcare facilities and otolaryngologists are lacking, and may encourage frequent testing of individuals in the high risk groups who are reluctant to have their blood tested. However, further validation in an independent cohort is required to strengthen the utility of this testing panel as a non-invasive detection tool for NPC. © 2019 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC
format	Article
author	Tan, Geok Wee Sivanesan, Vijaya Mohan Abdul Rahman, Farah Ida Hassan, Faridah Hasbullah, Harissa Husainy Ng, Ching Ching Khoo, Alan Soo Beng Tan, Lu Ping
author_facet	Tan, Geok Wee Sivanesan, Vijaya Mohan Abdul Rahman, Farah Ida Hassan, Faridah Hasbullah, Harissa Husainy Ng, Ching Ching Khoo, Alan Soo Beng Tan, Lu Ping
author_sort	Tan, Geok Wee
title	A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
title_short	A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
title_full	A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
title_fullStr	A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
title_full_unstemmed	A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
title_sort	novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma
publisher	John Wiley & Sons
publishDate	2019
url	http://eprints.um.edu.my/23309/ https://doi.org/10.1002/ijc.32173
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score	13.211869

A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma

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