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Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia

The intraspecific geographical venom variations of Calloselasma rhodostoma from Malaysia (CR-M), Indonesia (CR-I), Thailand (CR-T) and Vietnam (CR-V) were investigated through 1D SDS-PAGE and nano-ESI-LCMS/MS. The venom antigenicity, procoagulant activities and neutralization using Thai C. rhodostom...

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Main Authors: Tang, Esther Lai Har, Tan, Nget Hong, Fung, Shin Yee, Tan, Choo Hock
Format: Article
Published: Elsevier 2019
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/23282/
https://doi.org/10.1016/j.toxicon.2019.08.004
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spelling my.um.eprints.232822019-12-23T03:37:01Z http://eprints.um.edu.my/23282/ Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia Tang, Esther Lai Har Tan, Nget Hong Fung, Shin Yee Tan, Choo Hock R Medicine The intraspecific geographical venom variations of Calloselasma rhodostoma from Malaysia (CR-M), Indonesia (CR-I), Thailand (CR-T) and Vietnam (CR-V) were investigated through 1D SDS-PAGE and nano-ESI-LCMS/MS. The venom antigenicity, procoagulant activities and neutralization using Thai C. rhodostoma Monovalent Antivenom (CRMAV) were also investigated. SDS-PAGE patterns of the venoms were relatively similar with minor variations. Proteomic analysis revealed that snake venom metalloproteinases (SVMPs, particularly P–I class), serine proteases (SVSPs) and snaclecs dominated the venom protein composition (68.96–81.80%), followed by L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2) (7.37–11.08% and 5.18–13.81%, respectively), corroborating C. rhodostoma envenoming effects (hemorrhage, consumptive coagulopathy, thrombocytopenia and local tissue necrosis). Other proteins of lower abundances (2.82–9.13%) identified include cysteine-rich secretory proteins (CRISP), phospholipase B, phosphodiesterase, nerve growth factor, 5′-nucleotidase, aminopeptidase and hyaluronidase. All four venoms exhibited strong procoagulant effects which were neutralized by CRMAV to different extents. CRMAV immunoreactivity was high toward venoms of CR-M, CR-I and CR-T but relatively low for CR-V venom. Among the venom samples from different locales, CR-V venom proteome has the smallest SVMP composition while SVSP, PLA2 and phosphodiesterase were more abundant in the venom. These variations in C. rhodostoma venom protein composition could partly explain the differences seen in immunoreactivity. (198 words). © 2019 Elsevier 2019 Article PeerReviewed Tang, Esther Lai Har and Tan, Nget Hong and Fung, Shin Yee and Tan, Choo Hock (2019) Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia. Toxicon, 169. pp. 91-102. ISSN 0041-0101 https://doi.org/10.1016/j.toxicon.2019.08.004 doi:10.1016/j.toxicon.2019.08.004
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Tang, Esther Lai Har
Tan, Nget Hong
Fung, Shin Yee
Tan, Choo Hock
Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
description The intraspecific geographical venom variations of Calloselasma rhodostoma from Malaysia (CR-M), Indonesia (CR-I), Thailand (CR-T) and Vietnam (CR-V) were investigated through 1D SDS-PAGE and nano-ESI-LCMS/MS. The venom antigenicity, procoagulant activities and neutralization using Thai C. rhodostoma Monovalent Antivenom (CRMAV) were also investigated. SDS-PAGE patterns of the venoms were relatively similar with minor variations. Proteomic analysis revealed that snake venom metalloproteinases (SVMPs, particularly P–I class), serine proteases (SVSPs) and snaclecs dominated the venom protein composition (68.96–81.80%), followed by L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2) (7.37–11.08% and 5.18–13.81%, respectively), corroborating C. rhodostoma envenoming effects (hemorrhage, consumptive coagulopathy, thrombocytopenia and local tissue necrosis). Other proteins of lower abundances (2.82–9.13%) identified include cysteine-rich secretory proteins (CRISP), phospholipase B, phosphodiesterase, nerve growth factor, 5′-nucleotidase, aminopeptidase and hyaluronidase. All four venoms exhibited strong procoagulant effects which were neutralized by CRMAV to different extents. CRMAV immunoreactivity was high toward venoms of CR-M, CR-I and CR-T but relatively low for CR-V venom. Among the venom samples from different locales, CR-V venom proteome has the smallest SVMP composition while SVSP, PLA2 and phosphodiesterase were more abundant in the venom. These variations in C. rhodostoma venom protein composition could partly explain the differences seen in immunoreactivity. (198 words). © 2019
format Article
author Tang, Esther Lai Har
Tan, Nget Hong
Fung, Shin Yee
Tan, Choo Hock
author_facet Tang, Esther Lai Har
Tan, Nget Hong
Fung, Shin Yee
Tan, Choo Hock
author_sort Tang, Esther Lai Har
title Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
title_short Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
title_full Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
title_fullStr Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
title_full_unstemmed Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia
title_sort comparative proteomes, immunoreactivities and neutralization of procoagulant activities of calloselasma rhodostoma (malayan pit viper) venoms from four regions in southeast asia
publisher Elsevier
publishDate 2019
url http://eprints.um.edu.my/23282/
https://doi.org/10.1016/j.toxicon.2019.08.004
_version_ 1654960711894302720
score 13.222552

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