Investigation of possible rickettsial infection in patients with malaria
Rickettsioses are a common health problem in many geographical areas, including rural areas in Southeast Asia. Co-infection of rickettsioses and malaria has been reported in Africa, where common reservoir and vectors are available. In this study, blood samples of Malaysian patients microscopically p...
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my.um.eprints.232712019-12-23T02:00:43Z http://eprints.um.edu.my/23271/ Investigation of possible rickettsial infection in patients with malaria Tay, Sun Tee Kho, Kai Ling Vythilingam, Indra Ooi, Choo Huck Lau, Yee Ling R Medicine Rickettsioses are a common health problem in many geographical areas, including rural areas in Southeast Asia. Co-infection of rickettsioses and malaria has been reported in Africa, where common reservoir and vectors are available. In this study, blood samples of Malaysian patients microscopically positive (n=148) and negative (n=88) for malaria parasites (Plasmodium knowlesi, Plasmodium malariae, Plasmodium falciparum, and Plasmodium vivax) were screened for the presence of rickettsial DNA, using PCR assays targeting specific genes. A partial fragment of rickettsial ompB gene was successfully amplified and sequenced from a patient microscopically positive for Plasmodium spp. and PCR-positive for P. vivax. BLAST analysis of the ompB sequence demonstrated the highest sequence similarity (99.7% similarity, 408/409nt) with Rickettsia sp. RF2125 (Genbank accession no. JX183538) and 91.4% (374/409 nt) similarity with Rickettsia felis URRWXCal2 (Genbank accession no. CP000053). This study reports rickettsial infection in a malaria patient for the first time in the Southeast Asia region. © 2019, Malaysian Society for Parasitology. All rights reserved. Malaysian Society of Parasitology and Tropical Medicine 2019 Article PeerReviewed Tay, Sun Tee and Kho, Kai Ling and Vythilingam, Indra and Ooi, Choo Huck and Lau, Yee Ling (2019) Investigation of possible rickettsial infection in patients with malaria. Tropical Biomedicine, 36 (1). pp. 257-262. ISSN 0127-5720 http://msptm.org/files/Vol36No1/257-262-Tay-ST.pdf |
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Rickettsioses are a common health problem in many geographical areas, including rural areas in Southeast Asia. Co-infection of rickettsioses and malaria has been reported in Africa, where common reservoir and vectors are available. In this study, blood samples of Malaysian patients microscopically positive (n=148) and negative (n=88) for malaria parasites (Plasmodium knowlesi, Plasmodium malariae, Plasmodium falciparum, and Plasmodium vivax) were screened for the presence of rickettsial DNA, using PCR assays targeting specific genes. A partial fragment of rickettsial ompB gene was successfully amplified and sequenced from a patient microscopically positive for Plasmodium spp. and PCR-positive for P. vivax. BLAST analysis of the ompB sequence demonstrated the highest sequence similarity (99.7% similarity, 408/409nt) with Rickettsia sp. RF2125 (Genbank accession no. JX183538) and 91.4% (374/409 nt) similarity with Rickettsia felis URRWXCal2 (Genbank accession no. CP000053). This study reports rickettsial infection in a malaria patient for the first time in the Southeast Asia region. © 2019, Malaysian Society for Parasitology. All rights reserved. |
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Article |
author |
Tay, Sun Tee Kho, Kai Ling Vythilingam, Indra Ooi, Choo Huck Lau, Yee Ling |
author_facet |
Tay, Sun Tee Kho, Kai Ling Vythilingam, Indra Ooi, Choo Huck Lau, Yee Ling |
author_sort |
Tay, Sun Tee |
title |
Investigation of possible rickettsial infection in patients with malaria |
title_short |
Investigation of possible rickettsial infection in patients with malaria |
title_full |
Investigation of possible rickettsial infection in patients with malaria |
title_fullStr |
Investigation of possible rickettsial infection in patients with malaria |
title_full_unstemmed |
Investigation of possible rickettsial infection in patients with malaria |
title_sort |
investigation of possible rickettsial infection in patients with malaria |
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Malaysian Society of Parasitology and Tropical Medicine |
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2019 |
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http://eprints.um.edu.my/23271/ http://msptm.org/files/Vol36No1/257-262-Tay-ST.pdf |
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