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Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth

Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells...

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Main Authors:	Govindasamy , V., Abdullah , A.N., Ronald , V.S., Musa , S., Ab Aziz, Z. A. C., Zain, R.B, Totey, S., Bhonde , R.R., Abu Kasim, N.H.
Format:	Article
Language:	English
Published:	Lippincott, Williams & Wilkins 2010
Subjects:	R Medicine R Medicine (General) RK Dentistry
Online Access:	http://eprints.um.edu.my/2324/1/Inherent_Differential_Propensity_of_Dental_Pulp_Stem_Cells_Derived_from_Human_Deciduous_and_Permanent_Teeth.pdf http://eprints.um.edu.my/2324/ http://www.jendodon.com/article/S0099-2399(10)00460-7/abstract
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spelling	my.um.eprints.23242012-01-18T09:02:51Z http://eprints.um.edu.my/2324/ Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth Govindasamy , V. Abdullah , A.N. Ronald , V.S. Musa , S. Ab Aziz, Z. A. C. Zain, R.B Totey, S. Bhonde , R.R. Abu Kasim, N.H. R Medicine R Medicine (General) RK Dentistry Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineage-specific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies. Methods: We compared proliferation rate, gene expression profile, and lineage-specific propensity of stem cells derived from human deciduous (SCD) and permanent teeth (DPSCs) over 5 passages. Results: The proliferation rate of SCD was higher (cell number, 25 x 10(6) cells/mL; percent colony-forming units [CFUs], 151.67 +/- 10.5; percent cells in S/G2 phase, 12.4 +/- 1.48) than that of DPSCs (cell number, 21 x 10(6) cells/mL; percent CFUs, 133 +/- 17.62; percent cells in S/G2 phase, 10.4 +/- 1.18). It was observed that fold expression of several pluripotent markers such as OCT4, SOX2, NANOG, and REX1 were higher (>2) in SCD as compared with DPSCs. However, DPSCs showed higher expression of neuroectodermal markers PAX6, GBX2, and nestin (fold expression >100). Similarly, higher neurosphere formation and neuronal marker expression (NF, GFAP) were found in the differentiated DPSCs into neuron-like cells as compared with SCD. Conclusions: This study thus demonstrates that both SCD and DPSCs exhibit specific gene expression profile, with clear-cut inclination of DPSCs toward neuronal lineage. (J Endod 2010;36:1504-1515) Lippincott, Williams & Wilkins 2010-09 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2324/1/Inherent_Differential_Propensity_of_Dental_Pulp_Stem_Cells_Derived_from_Human_Deciduous_and_Permanent_Teeth.pdf Govindasamy , V. and Abdullah , A.N. and Ronald , V.S. and Musa , S. and Ab Aziz, Z. A. C. and Zain, R.B and Totey, S. and Bhonde , R.R. and Abu Kasim, N.H. (2010) Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth. Journal of Endodontics, 36 (9). pp. 1504-1515. ISSN 0099-2399 http://www.jendodon.com/article/S0099-2399(10)00460-7/abstract 10.1016/j.joen.2010.05.006
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
language	English
topic	R Medicine R Medicine (General) RK Dentistry
spellingShingle	R Medicine R Medicine (General) RK Dentistry Govindasamy , V. Abdullah , A.N. Ronald , V.S. Musa , S. Ab Aziz, Z. A. C. Zain, R.B Totey, S. Bhonde , R.R. Abu Kasim, N.H. Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
description	Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineage-specific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies. Methods: We compared proliferation rate, gene expression profile, and lineage-specific propensity of stem cells derived from human deciduous (SCD) and permanent teeth (DPSCs) over 5 passages. Results: The proliferation rate of SCD was higher (cell number, 25 x 10(6) cells/mL; percent colony-forming units [CFUs], 151.67 +/- 10.5; percent cells in S/G2 phase, 12.4 +/- 1.48) than that of DPSCs (cell number, 21 x 10(6) cells/mL; percent CFUs, 133 +/- 17.62; percent cells in S/G2 phase, 10.4 +/- 1.18). It was observed that fold expression of several pluripotent markers such as OCT4, SOX2, NANOG, and REX1 were higher (>2) in SCD as compared with DPSCs. However, DPSCs showed higher expression of neuroectodermal markers PAX6, GBX2, and nestin (fold expression >100). Similarly, higher neurosphere formation and neuronal marker expression (NF, GFAP) were found in the differentiated DPSCs into neuron-like cells as compared with SCD. Conclusions: This study thus demonstrates that both SCD and DPSCs exhibit specific gene expression profile, with clear-cut inclination of DPSCs toward neuronal lineage. (J Endod 2010;36:1504-1515)
format	Article
author	Govindasamy , V. Abdullah , A.N. Ronald , V.S. Musa , S. Ab Aziz, Z. A. C. Zain, R.B Totey, S. Bhonde , R.R. Abu Kasim, N.H.
author_facet	Govindasamy , V. Abdullah , A.N. Ronald , V.S. Musa , S. Ab Aziz, Z. A. C. Zain, R.B Totey, S. Bhonde , R.R. Abu Kasim, N.H.
author_sort	Govindasamy , V.
title	Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
title_short	Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
title_full	Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
title_fullStr	Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
title_full_unstemmed	Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
title_sort	inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth
publisher	Lippincott, Williams & Wilkins
publishDate	2010
url	http://eprints.um.edu.my/2324/1/Inherent_Differential_Propensity_of_Dental_Pulp_Stem_Cells_Derived_from_Human_Deciduous_and_Permanent_Teeth.pdf http://eprints.um.edu.my/2324/ http://www.jendodon.com/article/S0099-2399(10)00460-7/abstract
_version_	1643686900272201728
score	13.211869

Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth

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