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Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum

Background: Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Pha...

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Main Authors: Wu, Songcui, Gu, Wenhui, Huang, Aiyou, Li, Yuanxiang, Kumar, Manoj, Lim, Phaik Eem, Huan, Li, Gao, Shan, Wang, Guangce
Format: Article
Published: BMC 2019
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/23144/
https://doi.org/10.1186/s12934-019-1214-x
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spelling my.um.eprints.231442019-11-28T02:26:43Z http://eprints.um.edu.my/23144/ Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum Wu, Songcui Gu, Wenhui Huang, Aiyou Li, Yuanxiang Kumar, Manoj Lim, Phaik Eem Huan, Li Gao, Shan Wang, Guangce Q Science (General) QH Natural history Background: Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P.Tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results: In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P.Tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P.Tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions: The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P.Tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P.Tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production. © 2019 The Author(s). BMC 2019 Article PeerReviewed Wu, Songcui and Gu, Wenhui and Huang, Aiyou and Li, Yuanxiang and Kumar, Manoj and Lim, Phaik Eem and Huan, Li and Gao, Shan and Wang, Guangce (2019) Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum. Microbial Cell Factories, 18 (1). p. 161. ISSN 1475-2859 https://doi.org/10.1186/s12934-019-1214-x doi:10.1186/s12934-019-1214-x
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Wu, Songcui
Gu, Wenhui
Huang, Aiyou
Li, Yuanxiang
Kumar, Manoj
Lim, Phaik Eem
Huan, Li
Gao, Shan
Wang, Guangce
Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
description Background: Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P.Tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results: In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P.Tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P.Tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions: The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P.Tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P.Tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production. © 2019 The Author(s).
format Article
author Wu, Songcui
Gu, Wenhui
Huang, Aiyou
Li, Yuanxiang
Kumar, Manoj
Lim, Phaik Eem
Huan, Li
Gao, Shan
Wang, Guangce
author_facet Wu, Songcui
Gu, Wenhui
Huang, Aiyou
Li, Yuanxiang
Kumar, Manoj
Lim, Phaik Eem
Huan, Li
Gao, Shan
Wang, Guangce
author_sort Wu, Songcui
title Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
title_short Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
title_full Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
title_fullStr Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
title_full_unstemmed Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
title_sort elevated co2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered phaeodactylum tricornutum
publisher BMC
publishDate 2019
url http://eprints.um.edu.my/23144/
https://doi.org/10.1186/s12934-019-1214-x
_version_ 1651867406046330880
score 13.222552

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