Staff View: Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic efficiency, biochemical profiles and non-targeted metabolic profiling were studied to compare between the...

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Main Authors:	Yong, Wai Kuan, Lim, Phaik Eem, Vello, Vejeysri, Sim, Kae Shin, Majid, Nazia Abdul, Mustafa, Emienour Muzalina, Sulaiman, Nik Meriam Nik, Liew, Kan Ern, Chen, Brenna Jia Tian, Phang, Siew Moi
Format:	Article
Published:	Science Press 2019
Subjects:	Q Science (General) QH Natural history TP Chemical technology
Online Access:	http://eprints.um.edu.my/22960/ https://doi.org/10.1007/s00343-019-7263-5
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spelling	my.um.eprints.229602019-11-04T09:04:23Z http://eprints.um.edu.my/22960/ Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation Yong, Wai Kuan Lim, Phaik Eem Vello, Vejeysri Sim, Kae Shin Majid, Nazia Abdul Mustafa, Emienour Muzalina Sulaiman, Nik Meriam Nik Liew, Kan Ern Chen, Brenna Jia Tian Phang, Siew Moi Q Science (General) QH Natural history TP Chemical technology A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic efficiency, biochemical profiles and non-targeted metabolic profiling were studied to compare between the nitrogen-replete and deplete conditions. Slowed growth, change in photosynthetic pigments and lowered photosynthetic efficiency were observed in response to nitrogen deprivation. Biochemical profiles of the cultures showed an increased level of carbohydrate, lipids and total fatty acids, while the total soluble protein content was lowered. A trend of fatty acid saturation was observed in the nitrogen-deplete culture with an increase in the level of saturated fatty acids especially C16:0 and C18:0, accompanied by a decrease in proportions of monounsaturated and polyunsaturated fatty acids. Fifty-nine metabolites, including amino acids, lipids, phytochemical compounds, vitamins and cofactors were significantly dysregulated and annotated in this study. Pathway mapping analysis revealed a rewiring of metabolic pathways in the cells, particularly purine, carotenoid, nicotinate and nicotinamide, and amino acid metabolisms. Within the treatment period of nitrogen deprivation, the key processes involved were reshuffling of nitrogen from proteins and photosynthetic machinery, together with carbon repartitioning in carbohydrates and lipids. Science Press 2019 Article PeerReviewed Yong, Wai Kuan and Lim, Phaik Eem and Vello, Vejeysri and Sim, Kae Shin and Majid, Nazia Abdul and Mustafa, Emienour Muzalina and Sulaiman, Nik Meriam Nik and Liew, Kan Ern and Chen, Brenna Jia Tian and Phang, Siew Moi (2019) Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation. Journal of Oceanology and Limnology, 37 (1). pp. 186-198. ISSN 2096-5508 https://doi.org/10.1007/s00343-019-7263-5 doi:10.1007/s00343-019-7263-5
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH Natural history TP Chemical technology
spellingShingle	Q Science (General) QH Natural history TP Chemical technology Yong, Wai Kuan Lim, Phaik Eem Vello, Vejeysri Sim, Kae Shin Majid, Nazia Abdul Mustafa, Emienour Muzalina Sulaiman, Nik Meriam Nik Liew, Kan Ern Chen, Brenna Jia Tian Phang, Siew Moi Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
description	A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic efficiency, biochemical profiles and non-targeted metabolic profiling were studied to compare between the nitrogen-replete and deplete conditions. Slowed growth, change in photosynthetic pigments and lowered photosynthetic efficiency were observed in response to nitrogen deprivation. Biochemical profiles of the cultures showed an increased level of carbohydrate, lipids and total fatty acids, while the total soluble protein content was lowered. A trend of fatty acid saturation was observed in the nitrogen-deplete culture with an increase in the level of saturated fatty acids especially C16:0 and C18:0, accompanied by a decrease in proportions of monounsaturated and polyunsaturated fatty acids. Fifty-nine metabolites, including amino acids, lipids, phytochemical compounds, vitamins and cofactors were significantly dysregulated and annotated in this study. Pathway mapping analysis revealed a rewiring of metabolic pathways in the cells, particularly purine, carotenoid, nicotinate and nicotinamide, and amino acid metabolisms. Within the treatment period of nitrogen deprivation, the key processes involved were reshuffling of nitrogen from proteins and photosynthetic machinery, together with carbon repartitioning in carbohydrates and lipids.
format	Article
author	Yong, Wai Kuan Lim, Phaik Eem Vello, Vejeysri Sim, Kae Shin Majid, Nazia Abdul Mustafa, Emienour Muzalina Sulaiman, Nik Meriam Nik Liew, Kan Ern Chen, Brenna Jia Tian Phang, Siew Moi
author_facet	Yong, Wai Kuan Lim, Phaik Eem Vello, Vejeysri Sim, Kae Shin Majid, Nazia Abdul Mustafa, Emienour Muzalina Sulaiman, Nik Meriam Nik Liew, Kan Ern Chen, Brenna Jia Tian Phang, Siew Moi
author_sort	Yong, Wai Kuan
title	Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
title_short	Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
title_full	Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
title_fullStr	Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
title_full_unstemmed	Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation
title_sort	metabolic and physiological regulation of chlorella sp. (trebouxiophyceae, chlorophyta) under nitrogen deprivation
publisher	Science Press
publishDate	2019
url	http://eprints.um.edu.my/22960/ https://doi.org/10.1007/s00343-019-7263-5
_version_	1651867382191226880
score	13.222552

Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

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