Staff View: Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach

Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach

Carrageenan is a polysaccharide extracted from different species of red seaweed for use in pharmaceuticals and cosmetics industries. In Malaysia, κ-carrageenan is obtained through extraction from the tropical red seaweed Kappaphycus alvarezii. The use of tissue culture techniques in the propagation...

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Main Authors:	Usuldin, Siti Rokhiyah Ahmad, Al-Obaidi, Jameel R., Razali, Nurhanani, Junit, Sarni Mat, Ajang, Muhamad Johnny, Hussin, Siti Nahdatul Isnaini Said, Hamid, Shahlizah Sahul, Hanafi, Nursyuhaida Mohd, Roni, Ahmad Nor Hafzan Mat, Saleh, Norihan Mohd
Format:	Article
Published:	Springer Verlag 2017
Subjects:	R Medicine
Online Access:	http://eprints.um.edu.my/22860/ https://doi.org/10.1007/s10811-017-1119-1
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spelling	my.um.eprints.228602019-10-26T04:44:49Z http://eprints.um.edu.my/22860/ Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach Usuldin, Siti Rokhiyah Ahmad Al-Obaidi, Jameel R. Razali, Nurhanani Junit, Sarni Mat Ajang, Muhamad Johnny Hussin, Siti Nahdatul Isnaini Said Hamid, Shahlizah Sahul Hanafi, Nursyuhaida Mohd Roni, Ahmad Nor Hafzan Mat Saleh, Norihan Mohd R Medicine Carrageenan is a polysaccharide extracted from different species of red seaweed for use in pharmaceuticals and cosmetics industries. In Malaysia, κ-carrageenan is obtained through extraction from the tropical red seaweed Kappaphycus alvarezii. The use of tissue culture techniques in the propagation of K. alvarezii has proven to be effective in solving cultivation problems and produced high-quality seedlings and providing a sustainable source of better quality carrageenan. In order to understand the molecular mechanisms behind that, a proteomic investigation was conducted comparing the changes in protein expression in tissue-cultured and liquid-cultured K. alvarezii after 60 days of cultivation. Proteomic analysis was used to study the changes in the protein expression level between liquid-cultured and tissue-cultured of the red seaweed K. alvarezii. A total of 45 protein spots were found to be significantly different in their densities and three proteins, namely β-amylase, NAD-dependent sugar epimerase and B-phycoerythrin, showed a consistent pattern of upregulation in ELISA analyses, hence validating the 2-DE profiles. Changes in the proteins expression level were noticed in proteins related to energy production, metabolism and cellular maintenance. The protein changes in tissue-cultured seaweed possibly play an essential role in the production of carrageenan. Springer Verlag 2017 Article PeerReviewed Usuldin, Siti Rokhiyah Ahmad and Al-Obaidi, Jameel R. and Razali, Nurhanani and Junit, Sarni Mat and Ajang, Muhamad Johnny and Hussin, Siti Nahdatul Isnaini Said and Hamid, Shahlizah Sahul and Hanafi, Nursyuhaida Mohd and Roni, Ahmad Nor Hafzan Mat and Saleh, Norihan Mohd (2017) Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. Journal of Applied Phycology, 29 (4). pp. 1989-2001. ISSN 0921-8971 https://doi.org/10.1007/s10811-017-1119-1 doi:10.1007/s10811-017-1119-1
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine
spellingShingle	R Medicine Usuldin, Siti Rokhiyah Ahmad Al-Obaidi, Jameel R. Razali, Nurhanani Junit, Sarni Mat Ajang, Muhamad Johnny Hussin, Siti Nahdatul Isnaini Said Hamid, Shahlizah Sahul Hanafi, Nursyuhaida Mohd Roni, Ahmad Nor Hafzan Mat Saleh, Norihan Mohd Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
description	Carrageenan is a polysaccharide extracted from different species of red seaweed for use in pharmaceuticals and cosmetics industries. In Malaysia, κ-carrageenan is obtained through extraction from the tropical red seaweed Kappaphycus alvarezii. The use of tissue culture techniques in the propagation of K. alvarezii has proven to be effective in solving cultivation problems and produced high-quality seedlings and providing a sustainable source of better quality carrageenan. In order to understand the molecular mechanisms behind that, a proteomic investigation was conducted comparing the changes in protein expression in tissue-cultured and liquid-cultured K. alvarezii after 60 days of cultivation. Proteomic analysis was used to study the changes in the protein expression level between liquid-cultured and tissue-cultured of the red seaweed K. alvarezii. A total of 45 protein spots were found to be significantly different in their densities and three proteins, namely β-amylase, NAD-dependent sugar epimerase and B-phycoerythrin, showed a consistent pattern of upregulation in ELISA analyses, hence validating the 2-DE profiles. Changes in the proteins expression level were noticed in proteins related to energy production, metabolism and cellular maintenance. The protein changes in tissue-cultured seaweed possibly play an essential role in the production of carrageenan.
format	Article
author	Usuldin, Siti Rokhiyah Ahmad Al-Obaidi, Jameel R. Razali, Nurhanani Junit, Sarni Mat Ajang, Muhamad Johnny Hussin, Siti Nahdatul Isnaini Said Hamid, Shahlizah Sahul Hanafi, Nursyuhaida Mohd Roni, Ahmad Nor Hafzan Mat Saleh, Norihan Mohd
author_facet	Usuldin, Siti Rokhiyah Ahmad Al-Obaidi, Jameel R. Razali, Nurhanani Junit, Sarni Mat Ajang, Muhamad Johnny Hussin, Siti Nahdatul Isnaini Said Hamid, Shahlizah Sahul Hanafi, Nursyuhaida Mohd Roni, Ahmad Nor Hafzan Mat Saleh, Norihan Mohd
author_sort	Usuldin, Siti Rokhiyah Ahmad
title	Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
title_short	Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
title_full	Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
title_fullStr	Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
title_full_unstemmed	Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach
title_sort	molecular investigation of carrageenan production in kappaphycus alvarezii in different culture conditions: a proteomic approach
publisher	Springer Verlag
publishDate	2017
url	http://eprints.um.edu.my/22860/ https://doi.org/10.1007/s10811-017-1119-1
_version_	1648736233143140352
score	13.211869

Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach

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