Staff View: Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage...

Full description

Saved in:

Bibliographic Details
Main Authors:	Verusingam, Nalini Devi, Yeap, Swee Keong, Ky, Huynh, Paterson, Ian Charles, Khoo, Suan Phaik, Cheong, Soon Keng, Ong, Alan H.K., Kamarul, Tunku
Format:	Article
Published:	PeerJ 2017
Subjects:	R Medicine RK Dentistry
Online Access:	http://eprints.um.edu.my/22846/ https://doi.org/10.7717/peerj.3174
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.22846
record_format	eprints
spelling	my.um.eprints.228462019-10-24T06:54:44Z http://eprints.um.edu.my/22846/ Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming Verusingam, Nalini Devi Yeap, Swee Keong Ky, Huynh Paterson, Ian Charles Khoo, Suan Phaik Cheong, Soon Keng Ong, Alan H.K. Kamarul, Tunku R Medicine RK Dentistry Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity. Reprogrammed H103 (Rep-H103) exhibited similar ESCs morphologies with flatten cells and clear borders on feeder layer. Reprogrammed H376 (Rep-H376) did not show ESCs morphologies but grow with a disorganized morphology. Critical pluripotency genes Oct4, Sox2 and Nanog were expressed higher in Rep-H103 against the parental counterpart from passage 5 to passage 10. As for Rep-H376, Nanog expression against its parental counterpart showed a significant decrease at passage 5 and although increased in passage 10, the level of expression was similar to the parental cells. Rep-H103 exhibited pluripotent signals (Oct4, Sox2, Nanog and Tra-1-60) and could form EB with the presence of three germ layers markers. Rep-H103 displayed differentiation capacity into adipocytes and osteocytes. The OSCC cell line H103 which was able to be reprogrammed into an iPSC like state showed high expression of Oct4, Sox2 and Nanog at late passage and may provide a potential iPSC model to study multi-stage oncogenesis in OSCC. Subjects Cell Biology, Molecular Biology, Oncology, Medical Genetics. PeerJ 2017 Article PeerReviewed Verusingam, Nalini Devi and Yeap, Swee Keong and Ky, Huynh and Paterson, Ian Charles and Khoo, Suan Phaik and Cheong, Soon Keng and Ong, Alan H.K. and Kamarul, Tunku (2017) Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming. PeerJ, 5. e3174. ISSN 2167-8359 https://doi.org/10.7717/peerj.3174 doi:10.7717/peerj.3174
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine RK Dentistry
spellingShingle	R Medicine RK Dentistry Verusingam, Nalini Devi Yeap, Swee Keong Ky, Huynh Paterson, Ian Charles Khoo, Suan Phaik Cheong, Soon Keng Ong, Alan H.K. Kamarul, Tunku Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
description	Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity. Reprogrammed H103 (Rep-H103) exhibited similar ESCs morphologies with flatten cells and clear borders on feeder layer. Reprogrammed H376 (Rep-H376) did not show ESCs morphologies but grow with a disorganized morphology. Critical pluripotency genes Oct4, Sox2 and Nanog were expressed higher in Rep-H103 against the parental counterpart from passage 5 to passage 10. As for Rep-H376, Nanog expression against its parental counterpart showed a significant decrease at passage 5 and although increased in passage 10, the level of expression was similar to the parental cells. Rep-H103 exhibited pluripotent signals (Oct4, Sox2, Nanog and Tra-1-60) and could form EB with the presence of three germ layers markers. Rep-H103 displayed differentiation capacity into adipocytes and osteocytes. The OSCC cell line H103 which was able to be reprogrammed into an iPSC like state showed high expression of Oct4, Sox2 and Nanog at late passage and may provide a potential iPSC model to study multi-stage oncogenesis in OSCC. Subjects Cell Biology, Molecular Biology, Oncology, Medical Genetics.
format	Article
author	Verusingam, Nalini Devi Yeap, Swee Keong Ky, Huynh Paterson, Ian Charles Khoo, Suan Phaik Cheong, Soon Keng Ong, Alan H.K. Kamarul, Tunku
author_facet	Verusingam, Nalini Devi Yeap, Swee Keong Ky, Huynh Paterson, Ian Charles Khoo, Suan Phaik Cheong, Soon Keng Ong, Alan H.K. Kamarul, Tunku
author_sort	Verusingam, Nalini Devi
title	Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
title_short	Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
title_full	Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
title_fullStr	Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
title_full_unstemmed	Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming
title_sort	susceptibility of human oral squamous cell carcinoma (oscc) h103 and h376 cell lines to retroviral oskm mediated reprogramming
publisher	PeerJ
publishDate	2017
url	http://eprints.um.edu.my/22846/ https://doi.org/10.7717/peerj.3174
_version_	1648736229069422592
score	13.211869

Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

Similar Items